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The map label for this gene is purF [H]

Identifier: 218883835

GI number: 218883835

Start: 491186

End: 492466

Strand: Direct

Name: purF [H]

Synonym: DKAM_0524

Alternate gene names: 218883835

Gene position: 491186-492466 (Clockwise)

Preceding gene: 218883834

Following gene: 218883836

Centisome position: 35.98

GC content: 45.28

Gene sequence:

>1281_bases
GTGGTCTCAGGACTCTTATCCATATATGCATTCGACGAATACTGGGACGTAAGTAGCTTCGCGAGATACGGGTTACTGGC
TTTAAAGAATCGGGGAGACAACGCTGTTATCTGTTACTCTTTGAAAACAAGTATTGAGTGTATTGAGGCATCGATAGATG
AACTCGTGGGAAAAGGTCTTGAACACTCATCCAACATAGTTATTGCAGGCGTGTATAATGATAGGGCAAGCTATTATATT
GATGAAGCGGGCAGGGTAGCCATCCTGCTTGACAGGGAGTCTAATACCAATCTAGTACGGGATCTAGTAGAGTACATAGA
TAGCAATACGGGGATAAACGATATCATGAGGACCCTGAGTAATTCGGTGATTAATCCTCCAGTATTAATGAAGAAACATA
CCGGCATAATGCTACTTAGCAATAGCATAGTAGTAGCATGGAGAGGCCCTGAGTCGCTCTCACCTCTGGCGCTAGGTGGC
TACGGATTCGACATGGCTATTGTTGCATCTGAAACAGCCCCTATAGAAGTCGTGGGCGGGAATCCGAGGGCCGGTATACT
ACCGGGTCAACTTGTATTACTCAACAAATACGTTGTCAAGGTATTTGGTGCCAGGACGACTGGTAGGGTATGTCTGCTCG
AGCTCCTCTATACTGCAAGACCTGACTCGGTCATCGATGGCATACCTATATACATGTTTAGGAAGAAACTAGGGGAGAAG
CTTGCTGAGAAATTGATTAAGGAAGGCATTGGAACCAGTATCGATGTAGTTATAGGCGTGCCTGAGACAGCCCTTCCATA
CGCCCTTGGGATCTCTCAGAGACTTGGGAAACCCCTTGAACTCCTATTTGTCTCAACAGGTGTGAAGGCTCGTTCAATGT
TGAAGAATAGCTTAATGGAGAGGGCTATAGCGATACATTTGAAAATGAATCCCGTCAAAGGAGTTGTTGAGGGTAAGCGG
GTGCTTTTAGTTGATGACAGCGTGGTATCTGGTTCAACTCTTAAGACTGTGATTCAAGGCCTCCGCCAGAGACTCGGTGT
TGAAGAGGTTCATGTGGTGATTGCAAGCCCACCCATCAGGCTGCAATGCCCCTACTCCTTATTCTATTTCACTATGAATG
ATCTTATTTCAGCGAACATGGAGAGCGGGGACATGGTTAAATATCTTGACGCTGACTCTATAACATGGATACCAATAGAT
GTATTCGAAGAAGTCATGAAATCCTTCGGCGTTAATCCGTGTTTCTCATGTTTTATTGATAGCAGAGGGGATCATGCTTG
A

Upstream 100 bases:

>100_bases
GGGTTCGCCCAGTCTATGCATGGCTTGTTTTACAGGTAGATATGCTTTCAGGGGGCTTAGCCTCGAGGATCTTGAGAACA
TGGTTAAGAGGTGATGCATC

Downstream 100 bases:

>100_bases
AGATCCTGCTAATCGGATCCAGTGCAAGAGAACACGCCCTTGGATTAATGATGTTGAAGAGCGGGTTAGAGCCAAGGGTG
TATGTTGTATCGGAGTACAG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MVSGLLSIYAFDEYWDVSSFARYGLLALKNRGDNAVICYSLKTSIECIEASIDELVGKGLEHSSNIVIAGVYNDRASYYI
DEAGRVAILLDRESNTNLVRDLVEYIDSNTGINDIMRTLSNSVINPPVLMKKHTGIMLLSNSIVVAWRGPESLSPLALGG
YGFDMAIVASETAPIEVVGGNPRAGILPGQLVLLNKYVVKVFGARTTGRVCLLELLYTARPDSVIDGIPIYMFRKKLGEK
LAEKLIKEGIGTSIDVVIGVPETALPYALGISQRLGKPLELLFVSTGVKARSMLKNSLMERAIAIHLKMNPVKGVVEGKR
VLLVDDSVVSGSTLKTVIQGLRQRLGVEEVHVVIASPPIRLQCPYSLFYFTMNDLISANMESGDMVKYLDADSITWIPID
VFEEVMKSFGVNPCFSCFIDSRGDHA

Sequences:

>Translated_426_residues
MVSGLLSIYAFDEYWDVSSFARYGLLALKNRGDNAVICYSLKTSIECIEASIDELVGKGLEHSSNIVIAGVYNDRASYYI
DEAGRVAILLDRESNTNLVRDLVEYIDSNTGINDIMRTLSNSVINPPVLMKKHTGIMLLSNSIVVAWRGPESLSPLALGG
YGFDMAIVASETAPIEVVGGNPRAGILPGQLVLLNKYVVKVFGARTTGRVCLLELLYTARPDSVIDGIPIYMFRKKLGEK
LAEKLIKEGIGTSIDVVIGVPETALPYALGISQRLGKPLELLFVSTGVKARSMLKNSLMERAIAIHLKMNPVKGVVEGKR
VLLVDDSVVSGSTLKTVIQGLRQRLGVEEVHVVIASPPIRLQCPYSLFYFTMNDLISANMESGDMVKYLDADSITWIPID
VFEEVMKSFGVNPCFSCFIDSRGDHA
>Mature_426_residues
MVSGLLSIYAFDEYWDVSSFARYGLLALKNRGDNAVICYSLKTSIECIEASIDELVGKGLEHSSNIVIAGVYNDRASYYI
DEAGRVAILLDRESNTNLVRDLVEYIDSNTGINDIMRTLSNSVINPPVLMKKHTGIMLLSNSIVVAWRGPESLSPLALGG
YGFDMAIVASETAPIEVVGGNPRAGILPGQLVLLNKYVVKVFGARTTGRVCLLELLYTARPDSVIDGIPIYMFRKKLGEK
LAEKLIKEGIGTSIDVVIGVPETALPYALGISQRLGKPLELLFVSTGVKARSMLKNSLMERAIAIHLKMNPVKGVVEGKR
VLLVDDSVVSGSTLKTVIQGLRQRLGVEEVHVVIASPPIRLQCPYSLFYFTMNDLISANMESGDMVKYLDADSITWIPID
VFEEVMKSFGVNPCFSCFIDSRGDHA

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=337, Percent_Identity=25.2225519287834, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1788651, Length=260, Percent_Identity=31.5384615384615, Blast_Score=129, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17554892, Length=297, Percent_Identity=27.2727272727273, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323958, Length=283, Percent_Identity=29.3286219081272, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24659604, Length=269, Percent_Identity=28.2527881040892, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24659598, Length=269, Percent_Identity=28.2527881040892, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI28573187, Length=275, Percent_Identity=26.9090909090909, Blast_Score=102, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 46561; Mature: 46561

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSGLLSIYAFDEYWDVSSFARYGLLALKNRGDNAVICYSLKTSIECIEASIDELVGKGL
CCCCCEEEEEECCCCCHHHHHHHCEEEEECCCCCEEEEEEECCHHHHHHHHHHHHHCCCC
EHSSNIVIAGVYNDRASYYIDEAGRVAILLDRESNTNLVRDLVEYIDSNTGINDIMRTLS
CCCCCEEEEEEECCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHH
NSVINPPVLMKKHTGIMLLSNSIVVAWRGPESLSPLALGGYGFDMAIVASETAPIEVVGG
HCCCCCCEEEECCCCEEEEECCEEEEECCCCCCCCEEECCCCCEEEEEECCCCCEEEECC
NPRAGILPGQLVLLNKYVVKVFGARTTGRVCLLELLYTARPDSVIDGIPIYMFRKKLGEK
CCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHH
LAEKLIKEGIGTSIDVVIGVPETALPYALGISQRLGKPLELLFVSTGVKARSMLKNSLME
HHHHHHHHCCCCEEEEEEECCCHHHHHHHCCHHHHCCCEEEEEEECCHHHHHHHHHHHHH
RAIAIHLKMNPVKGVVEGKRVLLVDDSVVSGSTLKTVIQGLRQRLGVEEVHVVIASPPIR
HEEEEEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCEE
LQCPYSLFYFTMNDLISANMESGDMVKYLDADSITWIPIDVFEEVMKSFGVNPCFSCFID
EECCHHEEEEEHHHHHHCCCCCCCEEEEECCCCEEEEEHHHHHHHHHHCCCCHHHHHEEC
SRGDHA
CCCCCC
>Mature Secondary Structure
MVSGLLSIYAFDEYWDVSSFARYGLLALKNRGDNAVICYSLKTSIECIEASIDELVGKGL
CCCCCEEEEEECCCCCHHHHHHHCEEEEECCCCCEEEEEEECCHHHHHHHHHHHHHCCCC
EHSSNIVIAGVYNDRASYYIDEAGRVAILLDRESNTNLVRDLVEYIDSNTGINDIMRTLS
CCCCCEEEEEEECCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHH
NSVINPPVLMKKHTGIMLLSNSIVVAWRGPESLSPLALGGYGFDMAIVASETAPIEVVGG
HCCCCCCEEEECCCCEEEEECCEEEEECCCCCCCCEEECCCCCEEEEEECCCCCEEEECC
NPRAGILPGQLVLLNKYVVKVFGARTTGRVCLLELLYTARPDSVIDGIPIYMFRKKLGEK
CCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHH
LAEKLIKEGIGTSIDVVIGVPETALPYALGISQRLGKPLELLFVSTGVKARSMLKNSLME
HHHHHHHHCCCCEEEEEEECCCHHHHHHHCCHHHHCCCEEEEEEECCHHHHHHHHHHHHH
RAIAIHLKMNPVKGVVEGKRVLLVDDSVVSGSTLKTVIQGLRQRLGVEEVHVVIASPPIR
HEEEEEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCEE
LQCPYSLFYFTMNDLISANMESGDMVKYLDADSITWIPIDVFEEVMKSFGVNPCFSCFID
EECCHHEEEEEHHHHHHCCCCCCCEEEEECCCCEEEEEHHHHHHHHHHCCCCHHHHHEEC
SRGDHA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]