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The map label for this gene is purF [H]

Identifier: 218883834

GI number: 218883834

Start: 489809

End: 491179

Strand: Direct

Name: purF [H]

Synonym: DKAM_0523

Alternate gene names: 218883834

Gene position: 489809-491179 (Clockwise)

Preceding gene: 218883833

Following gene: 218883835

Centisome position: 35.88

GC content: 47.63

Gene sequence:

>1371_bases
ATGGGAAGAGGGGATCACTTCAGGATCCTGGTTGAGCTATTATTGGAGTTGCAGCATAGGGGACAGGAGGCTGCTGGCAT
AGTCTTATTCGATATGGAGGCTAAGAGGTTTGTAAGACTAGCTGGAAGTGGTGTAGTCGGTAACCTACTACTTAAAGACT
CAGCGAGAAATATATCCATAGAGTCCGCGCAGGGAAGATACTATGGTGGAGTAGGGCATGTACGCTATGCCACCACCGGT
GGATACTGGGGTGCTGTTATACAGCCGATTATAGTTAATACTGGGGGATTAAATTTCTCACTGGCTTTCAACGGGACAAT
AGCTAACTACAGGCTTATAGCCGATGAAATAAGGGTTAAGGCCTCCAATGACAGCAGGGTCCTAGGATACCTTATAAGTG
AGCTGAGCCGCGAATATAGGGGCGATATTGTAGAGGCTGCGAGACATTTAAGCGAGTATGTTATAGGTGGTTATAGTCTC
ATCGTGTTAACCAATGAGCCACGCATAATTATCGCTAGGGATCCAGCCGGCTACAGGCCATTATCGTACTCGCTTCACGG
GGAAGACTTCTACGTTGCCTCTGAGACAGCTGCCCTTGAAGTAATCGGTGCCGGAGAATGGAGTGAGGTAAAGCCTGGAG
AGGTTATTTCATTTGATGGCTTCTCTCTTGAGAAATATATGACTGGATCAGTTGGCACCCCGTATCCCTGCGTCTTCGAG
TACATATACTTTTCTAGACCGGACTCTGTTTTCAACGGGGTAAGTGTCTACGAGGCCAGATACCGTATGGGTAAGGAGCT
AGCTGGCTTAATCAATGGGATTGAAGTAGACATGGTTGTCCCGGTACCTGATACCGGCAGGATACCTGCACTAGGTCTTT
CAGAAACACTCGGACTCCACCTCGAGGAGGCTGTCATTGTCAACAAGTACATGGGCAGGGGCTTTATCACTCCTCCATCC
CATAGAAGCATGGTTGCCAAGTTGAAATACAATGTAGTGAGAAACAGAGTTATAGGTAAAAGAATACTCCTCGTTGAGGA
CTCGATAGTCAGAGGTACAACCCTCAATCACCTGGTGTCAAAGCTAAGGTATGCTGGTGCTTCAAAGGTTCATATAGGGG
TTGTTAGCCCACCCTTCAAATACCCATGTTTCATGGGTATAGATATCCCAGCAAAAAACGAGCTGATAGCTGGGGATCTG
ACCCCGGAGGAGGTAGCTAGAAGGCTTGGAGCCGACTCCGTAATATATAACACGATTGAAGGCCTTGGGAAAGCAGTGGG
TTCGCCCAGTCTATGCATGGCTTGTTTTACAGGTAGATATGCTTTCAGGGGGCTTAGCCTCGAGGATCTTGAGAACATGG
TTAAGAGGTGA

Upstream 100 bases:

>100_bases
ACTCGAGTTAGATATCGGTGTGCTCAGGGAGATCTATGCTGACTCCCTGGAGAAATACCTTGAGAGAGGCTAGTAATATA
TGTGTGGGATAGCCGCTTAC

Downstream 100 bases:

>100_bases
TGCATCGTGGTCTCAGGACTCTTATCCATATATGCATTCGACGAATACTGGGACGTAAGTAGCTTCGCGAGATACGGGTT
ACTGGCTTTAAAGAATCGGG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MGRGDHFRILVELLLELQHRGQEAAGIVLFDMEAKRFVRLAGSGVVGNLLLKDSARNISIESAQGRYYGGVGHVRYATTG
GYWGAVIQPIIVNTGGLNFSLAFNGTIANYRLIADEIRVKASNDSRVLGYLISELSREYRGDIVEAARHLSEYVIGGYSL
IVLTNEPRIIIARDPAGYRPLSYSLHGEDFYVASETAALEVIGAGEWSEVKPGEVISFDGFSLEKYMTGSVGTPYPCVFE
YIYFSRPDSVFNGVSVYEARYRMGKELAGLINGIEVDMVVPVPDTGRIPALGLSETLGLHLEEAVIVNKYMGRGFITPPS
HRSMVAKLKYNVVRNRVIGKRILLVEDSIVRGTTLNHLVSKLRYAGASKVHIGVVSPPFKYPCFMGIDIPAKNELIAGDL
TPEEVARRLGADSVIYNTIEGLGKAVGSPSLCMACFTGRYAFRGLSLEDLENMVKR

Sequences:

>Translated_456_residues
MGRGDHFRILVELLLELQHRGQEAAGIVLFDMEAKRFVRLAGSGVVGNLLLKDSARNISIESAQGRYYGGVGHVRYATTG
GYWGAVIQPIIVNTGGLNFSLAFNGTIANYRLIADEIRVKASNDSRVLGYLISELSREYRGDIVEAARHLSEYVIGGYSL
IVLTNEPRIIIARDPAGYRPLSYSLHGEDFYVASETAALEVIGAGEWSEVKPGEVISFDGFSLEKYMTGSVGTPYPCVFE
YIYFSRPDSVFNGVSVYEARYRMGKELAGLINGIEVDMVVPVPDTGRIPALGLSETLGLHLEEAVIVNKYMGRGFITPPS
HRSMVAKLKYNVVRNRVIGKRILLVEDSIVRGTTLNHLVSKLRYAGASKVHIGVVSPPFKYPCFMGIDIPAKNELIAGDL
TPEEVARRLGADSVIYNTIEGLGKAVGSPSLCMACFTGRYAFRGLSLEDLENMVKR
>Mature_455_residues
GRGDHFRILVELLLELQHRGQEAAGIVLFDMEAKRFVRLAGSGVVGNLLLKDSARNISIESAQGRYYGGVGHVRYATTGG
YWGAVIQPIIVNTGGLNFSLAFNGTIANYRLIADEIRVKASNDSRVLGYLISELSREYRGDIVEAARHLSEYVIGGYSLI
VLTNEPRIIIARDPAGYRPLSYSLHGEDFYVASETAALEVIGAGEWSEVKPGEVISFDGFSLEKYMTGSVGTPYPCVFEY
IYFSRPDSVFNGVSVYEARYRMGKELAGLINGIEVDMVVPVPDTGRIPALGLSETLGLHLEEAVIVNKYMGRGFITPPSH
RSMVAKLKYNVVRNRVIGKRILLVEDSIVRGTTLNHLVSKLRYAGASKVHIGVVSPPFKYPCFMGIDIPAKNELIAGDLT
PEEVARRLGADSVIYNTIEGLGKAVGSPSLCMACFTGRYAFRGLSLEDLENMVKR

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=441, Percent_Identity=35.6009070294785, Blast_Score=243, Evalue=4e-64,
Organism=Escherichia coli, GI1788651, Length=440, Percent_Identity=33.4090909090909, Blast_Score=207, Evalue=8e-55,
Organism=Caenorhabditis elegans, GI17554892, Length=473, Percent_Identity=34.2494714587738, Blast_Score=236, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6323958, Length=465, Percent_Identity=32.0430107526882, Blast_Score=200, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24659598, Length=468, Percent_Identity=35.042735042735, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24659604, Length=468, Percent_Identity=35.042735042735, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI28573187, Length=471, Percent_Identity=34.3949044585987, Blast_Score=233, Evalue=3e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 49974; Mature: 49843

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRGDHFRILVELLLELQHRGQEAAGIVLFDMEAKRFVRLAGSGVVGNLLLKDSARNISI
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHCCCCCCEEEEEEECCCCCEEE
ESAQGRYYGGVGHVRYATTGGYWGAVIQPIIVNTGGLNFSLAFNGTIANYRLIADEIRVK
ECCCCCEECCCCEEEEEECCCCCHHHEEEEEEECCCCEEEEEECCCEEEEEEEEEEEEEE
ASNDSRVLGYLISELSREYRGDIVEAARHLSEYVIGGYSLIVLTNEPRIIIARDPAGYRP
ECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCEEEEEECCCCCCC
LSYSLHGEDFYVASETAALEVIGAGEWSEVKPGEVISFDGFSLEKYMTGSVGTPYPCVFE
EEEEECCCEEEEEECCEEEEEEECCCCCCCCCCCEEEECCCEEHHEECCCCCCCCHHHHH
YIYFSRPDSVFNGVSVYEARYRMGKELAGLINGIEVDMVVPVPDTGRIPALGLSETLGLH
HHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHCCC
LEEAVIVNKYMGRGFITPPSHRSMVAKLKYNVVRNRVIGKRILLVEDSIVRGTTLNHLVS
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHH
KLRYAGASKVHIGVVSPPFKYPCFMGIDIPAKNELIAGDLTPEEVARRLGADSVIYNTIE
HHHHCCCCEEEEEEECCCCCCCEEEEECCCCCCCEEECCCCHHHHHHHHCCCHHHHHHHH
GLGKAVGSPSLCMACFTGRYAFRGLSLEDLENMVKR
HHHHHHCCCHHHHHHHHCCHHCCCCCHHHHHHHHCC
>Mature Secondary Structure 
GRGDHFRILVELLLELQHRGQEAAGIVLFDMEAKRFVRLAGSGVVGNLLLKDSARNISI
CCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHCCCCCCEEEEEEECCCCCEEE
ESAQGRYYGGVGHVRYATTGGYWGAVIQPIIVNTGGLNFSLAFNGTIANYRLIADEIRVK
ECCCCCEECCCCEEEEEECCCCCHHHEEEEEEECCCCEEEEEECCCEEEEEEEEEEEEEE
ASNDSRVLGYLISELSREYRGDIVEAARHLSEYVIGGYSLIVLTNEPRIIIARDPAGYRP
ECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCEEEEEECCCCCCC
LSYSLHGEDFYVASETAALEVIGAGEWSEVKPGEVISFDGFSLEKYMTGSVGTPYPCVFE
EEEEECCCEEEEEECCEEEEEEECCCCCCCCCCCEEEECCCEEHHEECCCCCCCCHHHHH
YIYFSRPDSVFNGVSVYEARYRMGKELAGLINGIEVDMVVPVPDTGRIPALGLSETLGLH
HHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHCCC
LEEAVIVNKYMGRGFITPPSHRSMVAKLKYNVVRNRVIGKRILLVEDSIVRGTTLNHLVS
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHH
KLRYAGASKVHIGVVSPPFKYPCFMGIDIPAKNELIAGDLTPEEVARRLGADSVIYNTIE
HHHHCCCCEEEEEEECCCCCCCEEEEECCCCCCCEEECCCCHHHHHHHHCCCHHHHHHHH
GLGKAVGSPSLCMACFTGRYAFRGLSLEDLENMVKR
HHHHHHCCCHHHHHHHHCCHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]