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The map label for this gene is mraW

Identifier: 218532980

GI number: 218532980

Start: 5469926

End: 5470987

Strand: Reverse

Name: mraW

Synonym: Mchl_5104

Alternate gene names: 218532980

Gene position: 5470987-5469926 (Counterclockwise)

Preceding gene: 218532981

Following gene: 218532979

Centisome position: 94.69

GC content: 73.35

Gene sequence:

>1062_bases
ATGCCGATGAGCCGGGCGCCGCGCACGGCCAGAGCCGAGCTGCCGAAGGGCGCGCAGGCTCGCCACGTTCCCGTCCTGCT
CGCCGAGGTTCTCGCCGCGCTCTCCCTCGACCGGCCGGGCCTGGCCGTGGACGGCACCTTCGGAGCGGGCGGCTACACCC
GCGCGCTGCTCGAGGCCGGGCCGGAGGTGCGCGTGATCGCCATCGATCGCGATCCCACCGCGATCCGGGGCGGAGCGGAC
CTCGTGAATGCCTTTGGGGGCCGCCTGCGTCTGGTGCAGGGCCGCTTCGGCGAACTCGACACGCTCATCGCCGATCAGAA
CGAGGCGCAGGCCGATTGGGTCGTGCTCGATATCGGCGTGTCCTCGATGCAGATCGACGAGGCGCAGCGCGGCTTCTCGT
TCCGGCAGGACGGGCCGCTCGACATGCGCATGGGCGGGGAGGGCCCCTCGGCGGCCGACCTCGTGAACGGCGAGGAAGAG
ACCACGCTCGCTGACATTCTCTACCATTTCGGCGAGGAGCGCCGCTCGCGGGCGGTGGCGCGCGCCATCGTCGAGGCGCG
GCGCCGCGCCCCGATCGTGACCACGGCGCAGCTCGCCGACCTCGTGGCCGGCGTGGTGCGGCCCGAGCCCGGCAGCCCGA
TCCATCCGGCGACCCGCAGCTTCCAGGGTCTGCGGATCGCGGTGAATGACGAACTCGGCGAATTGGTGCGCGGCCTGCAC
GCCGCCGAGCGGGTGCTCAAGCCCGGCGGCCGGCTCGCGGTGGTGACGTTCCATTCGCTGGAGGACCGCATCGTCAAGCA
GTTCTTCTCGGCCCGCAGCGGCCGGGCGGCGCAGGCCTCTCGGCACGTCCCAGGCGTGGAGCGGCCGGCCCCCAAGAGCT
TCAAGCTGGTGACCAAGGGTCCGGTGCTGCCCTCCGAGGCGGAGACGGACGTGAACCCGCGGTCGCGTTCGGCCAAGCTG
CGCGCCGGCGAGCGGACCGACGCGCCCGCGCCGCCGCCGCTGAGCGCCATCGAAACGCTGGCGAGCCTGCCGGCTCCACA
GGGACGGGGGCCACGGCGGTGA

Upstream 100 bases:

>100_bases
GGCCGCCCTGCGGTCCGTCCATTCAGCAGGCTCCCATGATGCATCCCCCGCCGGGTGGCGCCCGAGCTCTCTACGGATTC
CGCGAGAGCACCGTGGGGCG

Downstream 100 bases:

>100_bases
TCCGGCTTCTCAACGTGCTGGCGATCGTCGGGCTGATCTGCTCGGCGGTCTACGCCTACTCGATCAAGTACGACACGCTC
TATCAGGCGGGTCAGGTCTC

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MPMSRAPRTARAELPKGAQARHVPVLLAEVLAALSLDRPGLAVDGTFGAGGYTRALLEAGPEVRVIAIDRDPTAIRGGAD
LVNAFGGRLRLVQGRFGELDTLIADQNEAQADWVVLDIGVSSMQIDEAQRGFSFRQDGPLDMRMGGEGPSAADLVNGEEE
TTLADILYHFGEERRSRAVARAIVEARRRAPIVTTAQLADLVAGVVRPEPGSPIHPATRSFQGLRIAVNDELGELVRGLH
AAERVLKPGGRLAVVTFHSLEDRIVKQFFSARSGRAAQASRHVPGVERPAPKSFKLVTKGPVLPSEAETDVNPRSRSAKL
RAGERTDAPAPPPLSAIETLASLPAPQGRGPRR

Sequences:

>Translated_353_residues
MPMSRAPRTARAELPKGAQARHVPVLLAEVLAALSLDRPGLAVDGTFGAGGYTRALLEAGPEVRVIAIDRDPTAIRGGAD
LVNAFGGRLRLVQGRFGELDTLIADQNEAQADWVVLDIGVSSMQIDEAQRGFSFRQDGPLDMRMGGEGPSAADLVNGEEE
TTLADILYHFGEERRSRAVARAIVEARRRAPIVTTAQLADLVAGVVRPEPGSPIHPATRSFQGLRIAVNDELGELVRGLH
AAERVLKPGGRLAVVTFHSLEDRIVKQFFSARSGRAAQASRHVPGVERPAPKSFKLVTKGPVLPSEAETDVNPRSRSAKL
RAGERTDAPAPPPLSAIETLASLPAPQGRGPRR
>Mature_352_residues
PMSRAPRTARAELPKGAQARHVPVLLAEVLAALSLDRPGLAVDGTFGAGGYTRALLEAGPEVRVIAIDRDPTAIRGGADL
VNAFGGRLRLVQGRFGELDTLIADQNEAQADWVVLDIGVSSMQIDEAQRGFSFRQDGPLDMRMGGEGPSAADLVNGEEET
TLADILYHFGEERRSRAVARAIVEARRRAPIVTTAQLADLVAGVVRPEPGSPIHPATRSFQGLRIAVNDELGELVRGLHA
AERVLKPGGRLAVVTFHSLEDRIVKQFFSARSGRAAQASRHVPGVERPAPKSFKLVTKGPVLPSEAETDVNPRSRSAKLR
AGERTDAPAPPPLSAIETLASLPAPQGRGPRR

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=333, Percent_Identity=40.5405405405405, Blast_Score=231, Evalue=7e-61,
Organism=Homo sapiens, GI165377202, Length=188, Percent_Identity=42.0212765957447, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI1786270, Length=313, Percent_Identity=43.4504792332268, Blast_Score=225, Evalue=3e-60,
Organism=Drosophila melanogaster, GI62472493, Length=330, Percent_Identity=40.9090909090909, Blast_Score=211, Evalue=9e-55,
Organism=Drosophila melanogaster, GI28571637, Length=330, Percent_Identity=40.9090909090909, Blast_Score=211, Evalue=9e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_METC4 (B7KU77)

Other databases:

- EMBL:   CP001298
- RefSeq:   YP_002423796.1
- ProteinModelPortal:   B7KU77
- GeneID:   7118978
- GenomeReviews:   CP001298_GR
- KEGG:   mch:Mchl_5104
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 37673; Mature: 37542

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: NA

Important sites: BINDING 69-69 BINDING 96-96 BINDING 117-117 BINDING 124-124

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMSRAPRTARAELPKGAQARHVPVLLAEVLAALSLDRPGLAVDGTFGAGGYTRALLEAG
CCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHCC
PEVRVIAIDRDPTAIRGGADLVNAFGGRLRLVQGRFGELDTLIADQNEAQADWVVLDIGV
CCEEEEEECCCCCCCCCCHHHHHHCCCEEEEECCCCCCHHHHHCCCCCCCCCEEEEEECC
SSMQIDEAQRGFSFRQDGPLDMRMGGEGPSAADLVNGEEETTLADILYHFGEERRSRAVA
CHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
RAIVEARRRAPIVTTAQLADLVAGVVRPEPGSPIHPATRSFQGLRIAVNDELGELVRGLH
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCEEEEECHHHHHHHHHHH
AAERVLKPGGRLAVVTFHSLEDRIVKQFFSARSGRAAQASRHVPGVERPAPKSFKLVTKG
HHHHHHCCCCCEEEEEEHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCEEEEECC
PVLPSEAETDVNPRSRSAKLRAGERTDAPAPPPLSAIETLASLPAPQGRGPRR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PMSRAPRTARAELPKGAQARHVPVLLAEVLAALSLDRPGLAVDGTFGAGGYTRALLEAG
CCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHCC
PEVRVIAIDRDPTAIRGGADLVNAFGGRLRLVQGRFGELDTLIADQNEAQADWVVLDIGV
CCEEEEEECCCCCCCCCCHHHHHHCCCEEEEECCCCCCHHHHHCCCCCCCCCEEEEEECC
SSMQIDEAQRGFSFRQDGPLDMRMGGEGPSAADLVNGEEETTLADILYHFGEERRSRAVA
CHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
RAIVEARRRAPIVTTAQLADLVAGVVRPEPGSPIHPATRSFQGLRIAVNDELGELVRGLH
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCEEEEECHHHHHHHHHHH
AAERVLKPGGRLAVVTFHSLEDRIVKQFFSARSGRAAQASRHVPGVERPAPKSFKLVTKG
HHHHHHCCCCCEEEEEEHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCEEEEECC
PVLPSEAETDVNPRSRSAKLRAGERTDAPAPPPLSAIETLASLPAPQGRGPRR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA