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The map label for this gene is menE [H]

Identifier: 218532931

GI number: 218532931

Start: 5408023

End: 5409384

Strand: Reverse

Name: menE [H]

Synonym: Mchl_5054

Alternate gene names: 218532931

Gene position: 5409384-5408023 (Counterclockwise)

Preceding gene: 218532932

Following gene: 218532930

Centisome position: 93.62

GC content: 72.91

Gene sequence:

>1362_bases
ATGATCCTGCGCGAGAGACTTCTGGCGGCGGGCGGACTCGAGGGCCTGGTCCTGTCCGACTACCGCAGCAAAAAAGATCT
CGCCGGGATCGTCTCCGGCGAGCGCGACGCCGCCGCCACCGCGGCGCTCGCCGGCCGCCGGGTTCTGCTCGCGGTCGGCG
GGCAGATGGCGGCGGCCCGCGCCATGATCGCGCTCGACGGCCTCGCGGCTCGGCTCGCCCTCGTCCCCCCGAAGCTCGCG
CCGGAGCACATCGCCCGGATCGCATCGGATTCCGAGGCGGAGATCTGCGTCACCGATGCCGAGGGGCTGCCCCCCGACGC
CCTGCCGGGCCTGCCCCGGATCGACGCGGCCGAGGCGTTCCGCGCGGGCTGCGAGACATCGCGGGAGACGGAGTGGGCAT
TGGCCACCTCCGGCACCACCGGCGCGCCGAAGCTCGTCGCCCACACCCTCGAAGGCCTCGCCGGCGGCATCAACACCGCT
GCGGCGCCGGAGCCCGGCACGGTGTGGAGCACCTTCTACGACATCCGCCGCTACGGCGGCCTCCAAGTGTTCCTGCGGGC
GATGCTCGGGCCGTCCTCGCTCGTGCTCTCCGACGCGGGCGAGGCGGTGCGCGACTATCTCGTCCGCGTCGGTGCGGCGG
GCGTCACCCACATCCTCGGCACGCCCTCGCACTGGCGCCTCGCCCTGATGAGCGCGGCGCTCGACCGACTGAGCCCCCGC
TACGTGCGGCTCTCGGGCGAGATCGCCGACCAGAACATCCTCGACGCCCTGCGCGACGCCTTCCCTGAGGCGCGCATCGC
CCACGCCTACGCCTCGACCGAGGGCGGGGTCGGCTTCACCGTCGAGGACGGGCAGGAGGGTTTCCCGGCCGCGCTGATCG
GCACCCGCGCCGGGATCGAGATCACCGTGCGCGAGGACAGCCTGTTCGTGCGCTCCGGCCGAACCGGGCGGCGCTATCTC
GGCGCGGCGCCCGAGGCGCTGATCGCCGGGGACGGCTTCATCGACACCGGCGACCTCGTGGAGCGACGGGGCGAGCGCTA
CCACTTCCTCGGCCGGCGCAGCGGCGTGATCAATGTCGGCGGCGCCAAGGTGCAGCCGGAGGAGGTCGAAACCGTCATCA
ACGTCTTCCCCGGGGTCTCAATGTCCCGCGTGCGCGCCCGGCCCAACCCGATCCTCGGCGCGGTGGTCGAGGCGGAGGTG
GTGTTGCGTGCCGACGCGGGCACGGATCGGGCGGCGCTGAAGAAGGCGATCATCGCTCATTGTCGCCCGCGCCTGGCCGG
ACACAAGGTGCCGGCGAGCGTGAAATTCGTGGATGCGCTGCCGATCACGTCGGGCGGCAAGCTGGTGCGGAGGGGCGCGT
GA

Upstream 100 bases:

>100_bases
ACCCGTTCGGCGAGATGGATGCCTCCGACCTGCCGGTGACGGTCGGCGACCTCGTCGCCCTCTACGACCGCGCCGCCGCC
CGGCAGGCGGCCTGACCCCG

Downstream 100 bases:

>100_bases
GCGACGATCTCAAGACCGGACGCCTCGTCGTCGTCACGGGCGCGAGCCGGGGGCTCGGCCTCGCCATGGCCGAAACGCTC
GCCGCCGACGGGTTTCGCGT

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MILRERLLAAGGLEGLVLSDYRSKKDLAGIVSGERDAAATAALAGRRVLLAVGGQMAAARAMIALDGLAARLALVPPKLA
PEHIARIASDSEAEICVTDAEGLPPDALPGLPRIDAAEAFRAGCETSRETEWALATSGTTGAPKLVAHTLEGLAGGINTA
AAPEPGTVWSTFYDIRRYGGLQVFLRAMLGPSSLVLSDAGEAVRDYLVRVGAAGVTHILGTPSHWRLALMSAALDRLSPR
YVRLSGEIADQNILDALRDAFPEARIAHAYASTEGGVGFTVEDGQEGFPAALIGTRAGIEITVREDSLFVRSGRTGRRYL
GAAPEALIAGDGFIDTGDLVERRGERYHFLGRRSGVINVGGAKVQPEEVETVINVFPGVSMSRVRARPNPILGAVVEAEV
VLRADAGTDRAALKKAIIAHCRPRLAGHKVPASVKFVDALPITSGGKLVRRGA

Sequences:

>Translated_453_residues
MILRERLLAAGGLEGLVLSDYRSKKDLAGIVSGERDAAATAALAGRRVLLAVGGQMAAARAMIALDGLAARLALVPPKLA
PEHIARIASDSEAEICVTDAEGLPPDALPGLPRIDAAEAFRAGCETSRETEWALATSGTTGAPKLVAHTLEGLAGGINTA
AAPEPGTVWSTFYDIRRYGGLQVFLRAMLGPSSLVLSDAGEAVRDYLVRVGAAGVTHILGTPSHWRLALMSAALDRLSPR
YVRLSGEIADQNILDALRDAFPEARIAHAYASTEGGVGFTVEDGQEGFPAALIGTRAGIEITVREDSLFVRSGRTGRRYL
GAAPEALIAGDGFIDTGDLVERRGERYHFLGRRSGVINVGGAKVQPEEVETVINVFPGVSMSRVRARPNPILGAVVEAEV
VLRADAGTDRAALKKAIIAHCRPRLAGHKVPASVKFVDALPITSGGKLVRRGA
>Mature_453_residues
MILRERLLAAGGLEGLVLSDYRSKKDLAGIVSGERDAAATAALAGRRVLLAVGGQMAAARAMIALDGLAARLALVPPKLA
PEHIARIASDSEAEICVTDAEGLPPDALPGLPRIDAAEAFRAGCETSRETEWALATSGTTGAPKLVAHTLEGLAGGINTA
AAPEPGTVWSTFYDIRRYGGLQVFLRAMLGPSSLVLSDAGEAVRDYLVRVGAAGVTHILGTPSHWRLALMSAALDRLSPR
YVRLSGEIADQNILDALRDAFPEARIAHAYASTEGGVGFTVEDGQEGFPAALIGTRAGIEITVREDSLFVRSGRTGRRYL
GAAPEALIAGDGFIDTGDLVERRGERYHFLGRRSGVINVGGAKVQPEEVETVINVFPGVSMSRVRARPNPILGAVVEAEV
VLRADAGTDRAALKKAIIAHCRPRLAGHKVPASVKFVDALPITSGGKLVRRGA

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI115511026, Length=125, Percent_Identity=38.4, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI38505220, Length=345, Percent_Identity=27.536231884058, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI42544132, Length=123, Percent_Identity=37.3983739837398, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI122937307, Length=351, Percent_Identity=25.9259259259259, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI28416953, Length=370, Percent_Identity=26.4864864864865, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1788107, Length=125, Percent_Identity=32, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 47500; Mature: 47500

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILRERLLAAGGLEGLVLSDYRSKKDLAGIVSGERDAAATAALAGRRVLLAVGGQMAAAR
CCHHHHHHHHCCCCCEEECCCCCHHHHHHHHCCCCHHHHHHHHHCCEEEEEECCHHHHHH
AMIALDGLAARLALVPPKLAPEHIARIASDSEAEICVTDAEGLPPDALPGLPRIDAAEAF
HHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHH
RAGCETSRETEWALATSGTTGAPKLVAHTLEGLAGGINTAAAPEPGTVWSTFYDIRRYGG
HHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCC
LQVFLRAMLGPSSLVLSDAGEAVRDYLVRVGAAGVTHILGTPSHWRLALMSAALDRLSPR
HHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHCCCE
YVRLSGEIADQNILDALRDAFPEARIAHAYASTEGGVGFTVEDGQEGFPAALIGTRAGIE
EEEEECCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEECCCCCCCCEEEEECCCCEE
ITVREDSLFVRSGRTGRRYLGAAPEALIAGDGFIDTGDLVERRGERYHFLGRRSGVINVG
EEEECCEEEEECCCCCCHHHCCCCCEEEECCCCCCCCHHHHHCCCEEEEEECCCCEEECC
GAKVQPEEVETVINVFPGVSMSRVRARPNPILGAVVEAEVVLRADAGTDRAALKKAIIAH
CCCCCHHHHHHHHHHCCCCCHHHHHCCCCCCCHHEEEEEEEEEECCCCCHHHHHHHHHHH
CRPRLAGHKVPASVKFVDALPITSGGKLVRRGA
HCHHHCCCCCCCCEEEEEECCCCCCCCEEECCC
>Mature Secondary Structure
MILRERLLAAGGLEGLVLSDYRSKKDLAGIVSGERDAAATAALAGRRVLLAVGGQMAAAR
CCHHHHHHHHCCCCCEEECCCCCHHHHHHHHCCCCHHHHHHHHHCCEEEEEECCHHHHHH
AMIALDGLAARLALVPPKLAPEHIARIASDSEAEICVTDAEGLPPDALPGLPRIDAAEAF
HHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHH
RAGCETSRETEWALATSGTTGAPKLVAHTLEGLAGGINTAAAPEPGTVWSTFYDIRRYGG
HHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCC
LQVFLRAMLGPSSLVLSDAGEAVRDYLVRVGAAGVTHILGTPSHWRLALMSAALDRLSPR
HHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHCCCE
YVRLSGEIADQNILDALRDAFPEARIAHAYASTEGGVGFTVEDGQEGFPAALIGTRAGIE
EEEEECCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEECCCCCCCCEEEEECCCCEE
ITVREDSLFVRSGRTGRRYLGAAPEALIAGDGFIDTGDLVERRGERYHFLGRRSGVINVG
EEEECCEEEEECCCCCCHHHCCCCCEEEECCCCCCCCHHHHHCCCEEEEEECCCCEEECC
GAKVQPEEVETVINVFPGVSMSRVRARPNPILGAVVEAEVVLRADAGTDRAALKKAIIAH
CCCCCHHHHHHHHHHCCCCCHHHHHCCCCCCCHHEEEEEEEEEECCCCCHHHHHHHHHHH
CRPRLAGHKVPASVKFVDALPITSGGKLVRRGA
HCHHHCCCCCCCCEEEEEECCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA