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The map label for this gene is trpA

Identifier: 218532787

GI number: 218532787

Start: 5232080

End: 5232922

Strand: Reverse

Name: trpA

Synonym: Mchl_4910

Alternate gene names: 218532787

Gene position: 5232922-5232080 (Counterclockwise)

Preceding gene: 218532788

Following gene: 218532786

Centisome position: 90.57

GC content: 72.48

Gene sequence:

>843_bases
ATGACCGCCCGCATCGATGCCGCCTTCGCCCGCTGCCGCGCGGAAGGACGTGCCGCCCTCGTCACCTACGTGATGGCGGG
CGATCCCGATCCGGAGACCTCGCTTAAGGTGCTCGAAGCCCTACCGAAGGCGGGGGCCGACATCGTCGAGTTCGGCCTGC
CCTTCACCGATCCCATGGCCGACGGGCCGGCGATCCAGGCGGCGGGTCTGCGGGCGCTGAAGGCGGGCCAGGATCTGCGC
GGCACCCTCGCCCTCGTCCGCCGCTTCCGCGAAGGCGACGACCAGACGCCCGTGGTTCTGATGGGCTACTACAACCCGAT
CCATACCTACGGCGTGCCCCACTTCCTGGAGGATGCGCAGGCGGCCGGCATCGACGGTCTGATCGTCGTCGATCTGCCGC
CGGAGGAGGACGAGGAGCTCTGCCTGCCGGCGCTCGAAAAGGGGCTCGCCTTCATCCGCCTCGCGACCCCGACCACGGAC
GAGGCCCGCCTGCCGGCGGTTCTCGCGAACACCGCGGGCTTCGTCTACTACGTGTCGATCACCGGCGTGACCGGCACGGC
GACCCCCGATTTCGGCCGGGTGTCGCAGGCGGTCAGCCGGATCACGGCGCATACGAACCTGCCCGTCGTCGTCGGCTTCG
GCGTGAAGACCGGCGCGCACGCCGCCGAGATCGCCCGCGGCGCCGACGGCGTGGTGGTGGGCTCGGCCCTCGTCGATGCC
CTGGCCCGCAGCCTCGAACCCGGCGACCGGGCCGGGAGCGGCACGGTGGAGGCGGTCGCCTCCCTGGTGCGCGAGCTGGC
GCAGGGGGTCCGCAGCGCCGCCAAAGCGCCGGCCGGGGCCTGA

Upstream 100 bases:

>100_bases
GAGGAATTGGCCGGGGAAGTCGGGCCCTGCGCGGCGGCCCGGTTCCGGGGGAACCGTGCCCCGGGCCTCAAGGCCGGACC
TACCGCCGGGAGCGAGAACC

Downstream 100 bases:

>100_bases
CCGCAGCCGCTCAGCAGGGAGATCCCCTGGGATCGAGGCGGCGGCGATCCGATATCGATGTGGCAAGGGAACGCGGCAAG
TGAACGCGCTGGGACAGGGA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MTARIDAAFARCRAEGRAALVTYVMAGDPDPETSLKVLEALPKAGADIVEFGLPFTDPMADGPAIQAAGLRALKAGQDLR
GTLALVRRFREGDDQTPVVLMGYYNPIHTYGVPHFLEDAQAAGIDGLIVVDLPPEEDEELCLPALEKGLAFIRLATPTTD
EARLPAVLANTAGFVYYVSITGVTGTATPDFGRVSQAVSRITAHTNLPVVVGFGVKTGAHAAEIARGADGVVVGSALVDA
LARSLEPGDRAGSGTVEAVASLVRELAQGVRSAAKAPAGA

Sequences:

>Translated_280_residues
MTARIDAAFARCRAEGRAALVTYVMAGDPDPETSLKVLEALPKAGADIVEFGLPFTDPMADGPAIQAAGLRALKAGQDLR
GTLALVRRFREGDDQTPVVLMGYYNPIHTYGVPHFLEDAQAAGIDGLIVVDLPPEEDEELCLPALEKGLAFIRLATPTTD
EARLPAVLANTAGFVYYVSITGVTGTATPDFGRVSQAVSRITAHTNLPVVVGFGVKTGAHAAEIARGADGVVVGSALVDA
LARSLEPGDRAGSGTVEAVASLVRELAQGVRSAAKAPAGA
>Mature_279_residues
TARIDAAFARCRAEGRAALVTYVMAGDPDPETSLKVLEALPKAGADIVEFGLPFTDPMADGPAIQAAGLRALKAGQDLRG
TLALVRRFREGDDQTPVVLMGYYNPIHTYGVPHFLEDAQAAGIDGLIVVDLPPEEDEELCLPALEKGLAFIRLATPTTDE
ARLPAVLANTAGFVYYVSITGVTGTATPDFGRVSQAVSRITAHTNLPVVVGFGVKTGAHAAEIARGADGVVVGSALVDAL
ARSLEPGDRAGSGTVEAVASLVRELAQGVRSAAKAPAGA

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=212, Percent_Identity=32.5471698113208, Blast_Score=123, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6321412, Length=276, Percent_Identity=37.6811594202899, Blast_Score=181, Evalue=1e-46,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_METC4 (B7KSK7)

Other databases:

- EMBL:   CP001298
- RefSeq:   YP_002423603.1
- ProteinModelPortal:   B7KSK7
- SMR:   B7KSK7
- GeneID:   7114331
- GenomeReviews:   CP001298_GR
- KEGG:   mch:Mchl_4910
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28912; Mature: 28781

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 50-50 ACT_SITE 61-61

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARIDAAFARCRAEGRAALVTYVMAGDPDPETSLKVLEALPKAGADIVEFGLPFTDPMA
CCCCHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCC
DGPAIQAAGLRALKAGQDLRGTLALVRRFREGDDQTPVVLMGYYNPIHTYGVPHFLEDAQ
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHCCCCHHHHHHH
AAGIDGLIVVDLPPEEDEELCLPALEKGLAFIRLATPTTDEARLPAVLANTAGFVYYVSI
HCCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHCCCCEEEEEEE
TGVTGTATPDFGRVSQAVSRITAHTNLPVVVGFGVKTGAHAAEIARGADGVVVGSALVDA
ECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHCCCCCEEEHHHHHHH
LARSLEPGDRAGSGTVEAVASLVRELAQGVRSAAKAPAGA
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TARIDAAFARCRAEGRAALVTYVMAGDPDPETSLKVLEALPKAGADIVEFGLPFTDPMA
CCCHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCC
DGPAIQAAGLRALKAGQDLRGTLALVRRFREGDDQTPVVLMGYYNPIHTYGVPHFLEDAQ
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHCCCCHHHHHHH
AAGIDGLIVVDLPPEEDEELCLPALEKGLAFIRLATPTTDEARLPAVLANTAGFVYYVSI
HCCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHCCCCEEEEEEE
TGVTGTATPDFGRVSQAVSRITAHTNLPVVVGFGVKTGAHAAEIARGADGVVVGSALVDA
ECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHCCCCCEEEHHHHHHH
LARSLEPGDRAGSGTVEAVASLVRELAQGVRSAAKAPAGA
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA