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The map label for this gene is gacS [H]

Identifier: 218532769

GI number: 218532769

Start: 5203650

End: 5205752

Strand: Reverse

Name: gacS [H]

Synonym: Mchl_4892

Alternate gene names: 218532769

Gene position: 5205752-5203650 (Counterclockwise)

Preceding gene: 218532770

Following gene: 218532768

Centisome position: 90.1

GC content: 72.47

Gene sequence:

>2103_bases
ATGACCCTTGCGGTGAGCGTGATGGGCCTGAGCATCGCCGCCGCGATGCTGGCGCTGATCCTGGTGTCGCTCCGCCGCCG
CGAGCCCGTCGTGCCGGAATCCGACATGACGGAGGCGCTCCAGGACCGGCTCTGGCAGATCGCCGAGAGCGAGGAGCGCT
ACCGGGTGCTGGTCGAGGCCACGACCGAGGCCGTGGTTCAACGGGACGGGCAGGGGCGGATCACCTTCGCCAGCGCGGGG
TTTGCCGCGTTGCTCGGCATGAAGCCGCTGGAGTTGATCGGCTCGACGCTGAGCCCGCAGGTGATCGAGCGCGGCGCGAG
CGAGCAGCGCGCCGACGGCGTGCGGGTGGTCGAGGAGCGTCTGGTGCCGGTGGACGGGCTGCCGCGCTGGTTCTCCTTCA
TCGAGATGCCGGTCTCCGGCAGCGTTGACGGTCCGAACTGGCTGCGGGCCGGCCAGGACGTCACCGCGCGCGTCGAGGCG
GCGCGTGTCCTCGACGAGGCAAAGAGCCGGGCGGAGGCCGCCAACGTCGCCAAATCCCGCTTCCTCGCCACCGTCAGCCA
CGAGCTGCGCACGCCGTTGAACGGAATTCTCGGCATGGCCGACCTGCTGCTCGACACCCGGCTCGACCCCGAACAGCGCA
CCTATGTCGAGGCGTTCCGCACCAGCGGCAAGGCGCTGCTCGGCCTTGTCGACGGCATCCTCGATTTCTCCCGGATCGAG
GCCGGCCGCCTCGATCTGGCCGCCGAGCCCTTCGACGTCGCCGCCCTGGTCGAGGGCGTGGTCGAGCTGCTGGCGCCGCG
CGCGCAGGACAAGGGCCTGGAAATCGCCCTCGACATCGCCGACGACCTTGCCGCCCTGCGCGTGGGTGATGCCGACCGGG
TGCGGCAGATCCTCGTGAATCTGGCCGGCAACGCCATCAAGTTCACGCAGACCGGCGGCGTCGGCGTCAGCCTCGCCCGG
TCGGGGGAGGAGCAGGGGGAAGGGCTCGTCCTCACGGTCGAGGATACCGGGCCGGGCATTCCGGAGGAGCGCATCCCGAT
CCTGTTCGAGGAGTTCGAGCAGGGCGACGACAGCGCGAGCCACGAGGGCACCGGCCTCGGTCTGGCCATCACCCGCCGCC
TCGTGGAGCGCATGAACGGCACGATCGAGGCGCGCTCGACGATGGGGCGCGGCTCGACCTTCCGGGTCATGCTGCCGCTG
CCGGCGGCGGAGGGTGCGATGAGCCCCGAGACACCGAGCCTCGCACCGCGAAAGGTGCTGATCGTGGCGGCCTCGCCGTA
TCAGGCGCCGTTCCTGGCCCGGCGGCTCAGCCGCTCCGGGGCGGCGGCGGTGGTCGTGAACAGCGCGGAGGCCGGCCTCG
ATGCGCTGTCGGGCGTGGCCTTCGACGCGCTCATCGCCGACCGCAGCCTCGGCGACGCTGCCGTGCGGCGGCTCGCGGCC
GAGGCCGCCCGGAGCGGCGTGCGCTGCAGCCTGATCCTGCTCTCGCCCTTCGACCGGCGGGAATTCGGCGCCCCGAACGC
GGCGGGCTTCGACAGCTACCTGATCAAGCCGGTGCGCGCCCGCTCGCTGTTCGACCGCCTGCTGGAGCCGCGGCCCACTC
CGGCCCGCAGCCCGGCCGACGCGACGGCCCAGCCCGGTCCGGCCAAGACTGCATCGGGCAAGACTGCATCGGGCAAGACT
GCATCGAGCAAGACCGCACCCACCAAGACCGTCGCGCCGGGCCGCCGCGTGCTGCTGGCGGAGGACAACCCGATCAACGC
GCTGCTCGCCACCAAGGCGCTGGAGCGGCTCGGCGCGCAGGTGATCCACGCCCGCGACGGGCTCGAGGCTCTGGCGGCGG
CGGAAGGGCAGGGGCCGTTCGACCTCGCGTTGATCGACATCCGCATGCCCGGCCTCGACGGCCTGGAAACCGCCCGCCGC
ATCCGCGCCCGCGAGGCCGAGACCGGCGCGAGCCCGCTCCACCTCGTGGCGCTCACCGCCAATACCGGCCGCGAGGATGT
CGACGCGGCCTCCGCGGCCGGCTTCGACGGCTTCCTGCCCAAGCCGCTGAACCTGCGCGCCCTGCCGGCTCTGCTGGACC
GCCGCCAAGAGGAGGCAGCTTGA

Upstream 100 bases:

>100_bases
TTCTTTCTCAAGCGCCGCTTCACGCGCGGCTGGCACTGTCGCTCCCCTGACGAGCCGGTTTCCGGCCTCGGCCGGACTTT
GGGGGACCGACGTGGAACCG

Downstream 100 bases:

>100_bases
CAGGAGGCCGCTTGAGCGGCGGTCTGCGGGCCGCTTGAGGGGGGAAAGTCGGCGCCGGCCCTCGCGGCAGCTCTCGCGCT
GGTGCGAATTCTGCCTCATG

Product: PAS/PAC sensor hybrid histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 700; Mature: 699

Protein sequence:

>700_residues
MTLAVSVMGLSIAAAMLALILVSLRRREPVVPESDMTEALQDRLWQIAESEERYRVLVEATTEAVVQRDGQGRITFASAG
FAALLGMKPLELIGSTLSPQVIERGASEQRADGVRVVEERLVPVDGLPRWFSFIEMPVSGSVDGPNWLRAGQDVTARVEA
ARVLDEAKSRAEAANVAKSRFLATVSHELRTPLNGILGMADLLLDTRLDPEQRTYVEAFRTSGKALLGLVDGILDFSRIE
AGRLDLAAEPFDVAALVEGVVELLAPRAQDKGLEIALDIADDLAALRVGDADRVRQILVNLAGNAIKFTQTGGVGVSLAR
SGEEQGEGLVLTVEDTGPGIPEERIPILFEEFEQGDDSASHEGTGLGLAITRRLVERMNGTIEARSTMGRGSTFRVMLPL
PAAEGAMSPETPSLAPRKVLIVAASPYQAPFLARRLSRSGAAAVVVNSAEAGLDALSGVAFDALIADRSLGDAAVRRLAA
EAARSGVRCSLILLSPFDRREFGAPNAAGFDSYLIKPVRARSLFDRLLEPRPTPARSPADATAQPGPAKTASGKTASGKT
ASSKTAPTKTVAPGRRVLLAEDNPINALLATKALERLGAQVIHARDGLEALAAAEGQGPFDLALIDIRMPGLDGLETARR
IRAREAETGASPLHLVALTANTGREDVDAASAAGFDGFLPKPLNLRALPALLDRRQEEAA

Sequences:

>Translated_700_residues
MTLAVSVMGLSIAAAMLALILVSLRRREPVVPESDMTEALQDRLWQIAESEERYRVLVEATTEAVVQRDGQGRITFASAG
FAALLGMKPLELIGSTLSPQVIERGASEQRADGVRVVEERLVPVDGLPRWFSFIEMPVSGSVDGPNWLRAGQDVTARVEA
ARVLDEAKSRAEAANVAKSRFLATVSHELRTPLNGILGMADLLLDTRLDPEQRTYVEAFRTSGKALLGLVDGILDFSRIE
AGRLDLAAEPFDVAALVEGVVELLAPRAQDKGLEIALDIADDLAALRVGDADRVRQILVNLAGNAIKFTQTGGVGVSLAR
SGEEQGEGLVLTVEDTGPGIPEERIPILFEEFEQGDDSASHEGTGLGLAITRRLVERMNGTIEARSTMGRGSTFRVMLPL
PAAEGAMSPETPSLAPRKVLIVAASPYQAPFLARRLSRSGAAAVVVNSAEAGLDALSGVAFDALIADRSLGDAAVRRLAA
EAARSGVRCSLILLSPFDRREFGAPNAAGFDSYLIKPVRARSLFDRLLEPRPTPARSPADATAQPGPAKTASGKTASGKT
ASSKTAPTKTVAPGRRVLLAEDNPINALLATKALERLGAQVIHARDGLEALAAAEGQGPFDLALIDIRMPGLDGLETARR
IRAREAETGASPLHLVALTANTGREDVDAASAAGFDGFLPKPLNLRALPALLDRRQEEAA
>Mature_699_residues
TLAVSVMGLSIAAAMLALILVSLRRREPVVPESDMTEALQDRLWQIAESEERYRVLVEATTEAVVQRDGQGRITFASAGF
AALLGMKPLELIGSTLSPQVIERGASEQRADGVRVVEERLVPVDGLPRWFSFIEMPVSGSVDGPNWLRAGQDVTARVEAA
RVLDEAKSRAEAANVAKSRFLATVSHELRTPLNGILGMADLLLDTRLDPEQRTYVEAFRTSGKALLGLVDGILDFSRIEA
GRLDLAAEPFDVAALVEGVVELLAPRAQDKGLEIALDIADDLAALRVGDADRVRQILVNLAGNAIKFTQTGGVGVSLARS
GEEQGEGLVLTVEDTGPGIPEERIPILFEEFEQGDDSASHEGTGLGLAITRRLVERMNGTIEARSTMGRGSTFRVMLPLP
AAEGAMSPETPSLAPRKVLIVAASPYQAPFLARRLSRSGAAAVVVNSAEAGLDALSGVAFDALIADRSLGDAAVRRLAAE
AARSGVRCSLILLSPFDRREFGAPNAAGFDSYLIKPVRARSLFDRLLEPRPTPARSPADATAQPGPAKTASGKTASGKTA
SSKTAPTKTVAPGRRVLLAEDNPINALLATKALERLGAQVIHARDGLEALAAAEGQGPFDLALIDIRMPGLDGLETARRI
RAREAETGASPLHLVALTANTGREDVDAASAAGFDGFLPKPLNLRALPALLDRRQEEAA

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=610, Percent_Identity=31.9672131147541, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI145693157, Length=543, Percent_Identity=30.2025782688766, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI48994928, Length=449, Percent_Identity=30.7349665924276, Blast_Score=176, Evalue=6e-45,
Organism=Escherichia coli, GI87081816, Length=379, Percent_Identity=35.8839050131926, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI1788713, Length=498, Percent_Identity=27.710843373494, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI1786912, Length=251, Percent_Identity=32.2709163346614, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1788393, Length=277, Percent_Identity=31.4079422382672, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1786600, Length=226, Percent_Identity=32.7433628318584, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1790436, Length=237, Percent_Identity=29.1139240506329, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI87082128, Length=267, Percent_Identity=29.9625468164794, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1788549, Length=355, Percent_Identity=25.0704225352113, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789808, Length=227, Percent_Identity=29.9559471365639, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1786783, Length=239, Percent_Identity=26.3598326359833, Blast_Score=74, Evalue=5e-14,
Organism=Escherichia coli, GI1790346, Length=230, Percent_Identity=28.695652173913, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790300, Length=237, Percent_Identity=29.535864978903, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1790861, Length=228, Percent_Identity=27.1929824561404, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1787374, Length=153, Percent_Identity=29.4117647058824, Blast_Score=63, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=153, Percent_Identity=32.6797385620915, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 74138; Mature: 74007

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50112 PAS ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLAVSVMGLSIAAAMLALILVSLRRREPVVPESDMTEALQDRLWQIAESEERYRVLVEA
CEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEHH
TTEAVVQRDGQGRITFASAGFAALLGMKPLELIGSTLSPQVIERGASEQRADGVRVVEER
HHHHHHHCCCCCEEEEECCCHHHHHCCCHHHHHCCCCCHHHHHCCCCHHHHCCHHHHHHH
LVPVDGLPRWFSFIEMPVSGSVDGPNWLRAGQDVTARVEAARVLDEAKSRAEAANVAKSR
CCCCCCCHHHHHHHHCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLATVSHELRTPLNGILGMADLLLDTRLDPEQRTYVEAFRTSGKALLGLVDGILDFSRIE
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
AGRLDLAAEPFDVAALVEGVVELLAPRAQDKGLEIALDIADDLAALRVGDADRVRQILVN
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHCCCHHHHHHHHHH
LAGNAIKFTQTGGVGVSLARSGEEQGEGLVLTVEDTGPGIPEERIPILFEEFEQGDDSAS
HCCCEEEEEECCCCCEEEECCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCC
HEGTGLGLAITRRLVERMNGTIEARSTMGRGSTFRVMLPLPAAEGAMSPETPSLAPRKVL
CCCCCCHHHHHHHHHHHHCCCEEEHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEE
IVAASPYQAPFLARRLSRSGAAAVVVNSAEAGLDALSGVAFDALIADRSLGDAAVRRLAA
EEEECCCCCHHHHHHHCCCCCEEEEEECCHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
EAARSGVRCSLILLSPFDRREFGAPNAAGFDSYLIKPVRARSLFDRLLEPRPTPARSPAD
HHHHCCCEEEEEEECCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
ATAQPGPAKTASGKTASGKTASSKTAPTKTVAPGRRVLLAEDNPINALLATKALERLGAQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
VIHARDGLEALAAAEGQGPFDLALIDIRMPGLDGLETARRIRAREAETGASPLHLVALTA
HHHHHCCHHHHHHCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEC
NTGREDVDAASAAGFDGFLPKPLNLRALPALLDRRQEEAA
CCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLAVSVMGLSIAAAMLALILVSLRRREPVVPESDMTEALQDRLWQIAESEERYRVLVEA
EEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEHH
TTEAVVQRDGQGRITFASAGFAALLGMKPLELIGSTLSPQVIERGASEQRADGVRVVEER
HHHHHHHCCCCCEEEEECCCHHHHHCCCHHHHHCCCCCHHHHHCCCCHHHHCCHHHHHHH
LVPVDGLPRWFSFIEMPVSGSVDGPNWLRAGQDVTARVEAARVLDEAKSRAEAANVAKSR
CCCCCCCHHHHHHHHCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLATVSHELRTPLNGILGMADLLLDTRLDPEQRTYVEAFRTSGKALLGLVDGILDFSRIE
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
AGRLDLAAEPFDVAALVEGVVELLAPRAQDKGLEIALDIADDLAALRVGDADRVRQILVN
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHCCCHHHHHHHHHH
LAGNAIKFTQTGGVGVSLARSGEEQGEGLVLTVEDTGPGIPEERIPILFEEFEQGDDSAS
HCCCEEEEEECCCCCEEEECCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCC
HEGTGLGLAITRRLVERMNGTIEARSTMGRGSTFRVMLPLPAAEGAMSPETPSLAPRKVL
CCCCCCHHHHHHHHHHHHCCCEEEHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEE
IVAASPYQAPFLARRLSRSGAAAVVVNSAEAGLDALSGVAFDALIADRSLGDAAVRRLAA
EEEECCCCCHHHHHHHCCCCCEEEEEECCHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
EAARSGVRCSLILLSPFDRREFGAPNAAGFDSYLIKPVRARSLFDRLLEPRPTPARSPAD
HHHHCCCEEEEEEECCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
ATAQPGPAKTASGKTASGKTASSKTAPTKTVAPGRRVLLAEDNPINALLATKALERLGAQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
VIHARDGLEALAAAEGQGPFDLALIDIRMPGLDGLETARRIRAREAETGASPLHLVALTA
HHHHHCCHHHHHHCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEC
NTGREDVDAASAAGFDGFLPKPLNLRALPALLDRRQEEAA
CCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]