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The map label for this gene is otsB [H]

Identifier: 218532740

GI number: 218532740

Start: 5175777

End: 5176532

Strand: Reverse

Name: otsB [H]

Synonym: Mchl_4863

Alternate gene names: 218532740

Gene position: 5176532-5175777 (Counterclockwise)

Preceding gene: 218532749

Following gene: 218532739

Centisome position: 89.59

GC content: 72.35

Gene sequence:

>756_bases
GTGAGCGAGACCGCAGACTACGCCCTGTTCCTCGATTTCGACGGCACCCTGGTCGAGATCGCCCCGCGCCCCGACGCGGT
GCAGGTCGATCCCACCCTCGTGCCCGCGCTTGAGCGGTTGCGCGAACGGCTCGGCGGCGCGCTCGCCATCGTCACCGGAC
GGCCGGTCGCGGTGATCGATGATTTCCTGAGCCCCGCCCGGTTCGACGTGGCCGGGCTGCACGGCGTCGAGCGCCGAGTG
GACGGCACCTTGAGCGGCGGACGGCCGGAGGATCATCCGGACCTGCGCGCGGGCGTCGAGCGGCTGCACGCTGAGGCGGC
CCGCTACGAATCCGTACTGATCGAGGACAAGGGCGCCTCGGTCGCCGTGCATTGGCGGCTCGCCACGCCGGGCGACGCGC
AGGCGGCCGAGACCATCGTGAAGGCGGTGGCGGCCGATCTCGGCTCCGCCTACCGGCTTCAACTCGGCAAGGCGGTCGGC
GAAATCGTGCCGGCCGACGCCACCAAGGGCCACGCGATACGCGCCTTCCTGGAAGCAGCCCCCTATGCCGGCCGGCGCGC
GATCTTCCTCGGCGACGACCGCACCGACGAGATCGCCTTCGCCTCCGTCAACGAGGATGGCGGCATCAGCGTGCGCATCG
GCGACGGAGAGACCGTGGCCGGCCGCCGCATCGCTACTCCCGCGGATGTGCGCGCGCTGATCAGCGCCTGGGCCGAGGGC
GCGCCGATCGACCCGGACACGCTGCCCGCCGCCTGA

Upstream 100 bases:

>100_bases
GGGATGGGCCGCACGGGCGCCTCCGTTGGTGTCGCATCAGCGGTTTGTCGCAACCGCCCCTCATGCTAGCGCATGGGCAG
CCCGAGAGAAGGACGACGCC

Downstream 100 bases:

>100_bases
CATCGCTCACCGCTTCGTTCACCGCTTGCCGAACGGCCGACGCACCCTCTGTTAAGGTCGCGGCAAAGCACCACGAGAGG
CCAGAGGCGGATGTTCGAAT

Product: HAD-superfamily hydrolase, subfamily IIB

Products: NA

Alternate protein names: TPP; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase [H]

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MSETADYALFLDFDGTLVEIAPRPDAVQVDPTLVPALERLRERLGGALAIVTGRPVAVIDDFLSPARFDVAGLHGVERRV
DGTLSGGRPEDHPDLRAGVERLHAEAARYESVLIEDKGASVAVHWRLATPGDAQAAETIVKAVAADLGSAYRLQLGKAVG
EIVPADATKGHAIRAFLEAAPYAGRRAIFLGDDRTDEIAFASVNEDGGISVRIGDGETVAGRRIATPADVRALISAWAEG
APIDPDTLPAA

Sequences:

>Translated_251_residues
MSETADYALFLDFDGTLVEIAPRPDAVQVDPTLVPALERLRERLGGALAIVTGRPVAVIDDFLSPARFDVAGLHGVERRV
DGTLSGGRPEDHPDLRAGVERLHAEAARYESVLIEDKGASVAVHWRLATPGDAQAAETIVKAVAADLGSAYRLQLGKAVG
EIVPADATKGHAIRAFLEAAPYAGRRAIFLGDDRTDEIAFASVNEDGGISVRIGDGETVAGRRIATPADVRALISAWAEG
APIDPDTLPAA
>Mature_250_residues
SETADYALFLDFDGTLVEIAPRPDAVQVDPTLVPALERLRERLGGALAIVTGRPVAVIDDFLSPARFDVAGLHGVERRVD
GTLSGGRPEDHPDLRAGVERLHAEAARYESVLIEDKGASVAVHWRLATPGDAQAAETIVKAVAADLGSAYRLQLGKAVGE
IVPADATKGHAIRAFLEAAPYAGRRAIFLGDDRTDEIAFASVNEDGGISVRIGDGETVAGRRIATPADVRALISAWAEGA
PIDPDTLPAA

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=234, Percent_Identity=41.4529914529915, Blast_Score=151, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 26385; Mature: 26254

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETADYALFLDFDGTLVEIAPRPDAVQVDPTLVPALERLRERLGGALAIVTGRPVAVID
CCCCCCEEEEEECCCEEEEEECCCCCEEECCHHHHHHHHHHHHHCCEEEEEECCCHHHHH
DFLSPARFDVAGLHGVERRVDGTLSGGRPEDHPDLRAGVERLHAEAARYESVLIEDKGAS
HHCCCCCCCHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCE
VAVHWRLATPGDAQAAETIVKAVAADLGSAYRLQLGKAVGEIVPADATKGHAIRAFLEAA
EEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHC
PYAGRRAIFLGDDRTDEIAFASVNEDGGISVRIGDGETVAGRRIATPADVRALISAWAEG
CCCCCEEEEECCCCCCCEEEEEECCCCCEEEEECCCCEECCCCCCCHHHHHHHHHHHHCC
APIDPDTLPAA
CCCCCCCCCCC
>Mature Secondary Structure 
SETADYALFLDFDGTLVEIAPRPDAVQVDPTLVPALERLRERLGGALAIVTGRPVAVID
CCCCCEEEEEECCCEEEEEECCCCCEEECCHHHHHHHHHHHHHCCEEEEEECCCHHHHH
DFLSPARFDVAGLHGVERRVDGTLSGGRPEDHPDLRAGVERLHAEAARYESVLIEDKGAS
HHCCCCCCCHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCE
VAVHWRLATPGDAQAAETIVKAVAADLGSAYRLQLGKAVGEIVPADATKGHAIRAFLEAA
EEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHC
PYAGRRAIFLGDDRTDEIAFASVNEDGGISVRIGDGETVAGRRIATPADVRALISAWAEG
CCCCCEEEEECCCCCCCEEEEEECCCCCEEEEECCCCEECCCCCCCHHHHHHHHHHHHCC
APIDPDTLPAA
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]