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The map label for this gene is bhuA [H]

Identifier: 218710359

GI number: 218710359

Start: 2564305

End: 2566335

Strand: Reverse

Name: bhuA [H]

Synonym: VS_2395

Alternate gene names: 218710359

Gene position: 2566335-2564305 (Counterclockwise)

Preceding gene: 218710360

Following gene: 218710353

Centisome position: 77.78

GC content: 44.36

Gene sequence:

>2031_bases
ATGAAACTTAAAGCACTATCAGTAGCCGTTACGGTTGCCTTAACCTCATTTACAGCATTTGCTGCCGACGAACCAGAAAC
CGTAGTCGTTATCGGTTCTGCCCTTGACCAATTGATTCAGACTGAAATCAACTCTGACACACTGGAAAAGAAGCAAGCAT
CAGACATCAAAGATGTTTTGAATACTATGCCTAGCGTTACGGTTGACGGGAACGCACGTTACTCTCGCAAAGTATTTGTA
CGTGGTATGGAAGACAAATTTGCCGTAGTGACTATCGATGGTGCGCGACAAGAAGGTCAGCTTTTCCACCACTCTGGTGA
CCAAGCGATTGATCCTGCAATGCTAAAAAGAGCTGAAATTGAATTAGGTGGTAACTCTGCACTGTCAGGTTCTGGTGCTA
TCAATGGTTCATTTAGCTACGAAACGAAAGACCCAAGCGATCTTCTTAAGCCGGGTGAAAGCGTTGGTGCTCGAGTAAAA
ACAAGCTATCAAACAGCTTATGAGCGTTTTGCAACCAACGTTGCTGTGTATGCAAAAGTGAACGACAAGCTACAGTTCAT
GGGTATTGCGAACTATTCAGAAGATGGCGATCTGCACATTCCTGATCAAGAAGCGGTAACCTCTAAGCAGGGTGAGCTTA
AGTCGGGTTTGGCTAAAGTTGTTTTCATTCCTAATGATGCCAATGAATTTAAACTGACTTTCAACCGATACAGTGATGGT
GGTACGCGTCAGCTTTCTGGCGAAAAGGCGGGTGCTCTGTATGCTGACGATAAACACGACTATCATTCGCTTAATCGCGA
TACAGTGACGCTACAGCACGAATACGATGCAGGTAGTGATCTAGTTCATCTGAAAAGTAATGTTTACTACAACCGTCAAT
ACATGGATCGTGATGCTCTTGAAGGTACCGCTTGGGGCAAAACACCGGCAGGTAAATGGTTCAAAGATGGCAAAATGTCT
TACCCTAAGCGTGAATACAATGTAACAACCATTGGCGGCGACATTCGTAACGTTTCTTGGGTTGGTGAGCATGAGCTTAC
TTATGGTCTTGAGGGCTACAAGGCCAAGCAATGGATTGATGCTGGTCAAGGTGTATATCTAAGCGGTACTAAGCAAGGTC
AAACCGAGAACTACAGCATGGATGGTGGTACGGTGACGGCTTATGGTCTGTATCTGCAAGATATGTACGAGTTCAGCGAC
TTCCGTTTAACAACGGGCCTTCGTTACGACGTTCATAAACTGGGTGGTGTGTATAAGGGCGAGTTTGACCAACTATCTCC
AAAGTTCAAAGGTGAATACCAAACAACGGAAAATCTAAAACTTCGTGTGGGTTACGGTCGTCTGTTTAAAGGCGCGTCAC
TGCCGGAAACGCTAACGATGAAAGCACCAAGCAAAGTTAAGCAGTCTGATACGAAGGCGATGACGGGTAATAACTATGAA
GCGGGCTTTGATTATGAGCTAACACGTTTGCTTGCGGCTGATTACGCGATTCTAGGTTTCACGGCTTATACCTACGACCT
TGATAACCAAATGCACCCAACTAAGAACAACGCAACATTATCTAATAAGTATGATGTTGAAGTTTGGGGGGTAGAGACTG
TATTGAGCTATCAGTTAGATGCTTTAAGTGTTTACGCTAACCACTCTTACTCAGATGGTGAGCAAAAGAGCCTGAAAAAC
GGCAAGACAAGTCACATGAATAAAACCGGTATTCATAACTTTAAGGCCGGTTTTAACTACAGCCTAAACAATGAATTTGT
GTTTGGTTGGGATTCACGCTTTGTTCCGGGGAACGATTACGAGAACGAAAAAGGCAAAGAGATCAAACGCTCAGGCTTCG
CAACGCACAACATTTGGGCGGCTTACACACCGACGTTTGCTAAAGACCTAGAGATGAACCTTGGTATCGATAACCTGCTT
GATAAGAAGTACGCTGAGCATACTGGTTTTGGTATCTCTTGGGGTTCAGAAAAGTACACCAGCTATGAAGCGGGTCGTAA
CTTTAAAGCTTCTATCGCTTATAGCTTCTAA

Upstream 100 bases:

>100_bases
AGTAAAGAATGTAAATGATAACCATTACTATTAACATTGTTATTAGTGTCGTGTACCATTCGTGCACTTTCACTTAAATC
TATAAAATATAAGTCGCATT

Downstream 100 bases:

>100_bases
TCTGCTCTTAGCTGAAAGTTATTCTAGAAATAGAGAGTTATCAGTATAAGTGCAATGTTTCGTCTTAAAATGAAACAACA
AAAAAGCCTTAGTTCAGACT

Product: hypothetical protein

Products: NA

Alternate protein names: Brucella heme uptake protein A [H]

Number of amino acids: Translated: 676; Mature: 676

Protein sequence:

>676_residues
MKLKALSVAVTVALTSFTAFAADEPETVVVIGSALDQLIQTEINSDTLEKKQASDIKDVLNTMPSVTVDGNARYSRKVFV
RGMEDKFAVVTIDGARQEGQLFHHSGDQAIDPAMLKRAEIELGGNSALSGSGAINGSFSYETKDPSDLLKPGESVGARVK
TSYQTAYERFATNVAVYAKVNDKLQFMGIANYSEDGDLHIPDQEAVTSKQGELKSGLAKVVFIPNDANEFKLTFNRYSDG
GTRQLSGEKAGALYADDKHDYHSLNRDTVTLQHEYDAGSDLVHLKSNVYYNRQYMDRDALEGTAWGKTPAGKWFKDGKMS
YPKREYNVTTIGGDIRNVSWVGEHELTYGLEGYKAKQWIDAGQGVYLSGTKQGQTENYSMDGGTVTAYGLYLQDMYEFSD
FRLTTGLRYDVHKLGGVYKGEFDQLSPKFKGEYQTTENLKLRVGYGRLFKGASLPETLTMKAPSKVKQSDTKAMTGNNYE
AGFDYELTRLLAADYAILGFTAYTYDLDNQMHPTKNNATLSNKYDVEVWGVETVLSYQLDALSVYANHSYSDGEQKSLKN
GKTSHMNKTGIHNFKAGFNYSLNNEFVFGWDSRFVPGNDYENEKGKEIKRSGFATHNIWAAYTPTFAKDLEMNLGIDNLL
DKKYAEHTGFGISWGSEKYTSYEAGRNFKASIAYSF

Sequences:

>Translated_676_residues
MKLKALSVAVTVALTSFTAFAADEPETVVVIGSALDQLIQTEINSDTLEKKQASDIKDVLNTMPSVTVDGNARYSRKVFV
RGMEDKFAVVTIDGARQEGQLFHHSGDQAIDPAMLKRAEIELGGNSALSGSGAINGSFSYETKDPSDLLKPGESVGARVK
TSYQTAYERFATNVAVYAKVNDKLQFMGIANYSEDGDLHIPDQEAVTSKQGELKSGLAKVVFIPNDANEFKLTFNRYSDG
GTRQLSGEKAGALYADDKHDYHSLNRDTVTLQHEYDAGSDLVHLKSNVYYNRQYMDRDALEGTAWGKTPAGKWFKDGKMS
YPKREYNVTTIGGDIRNVSWVGEHELTYGLEGYKAKQWIDAGQGVYLSGTKQGQTENYSMDGGTVTAYGLYLQDMYEFSD
FRLTTGLRYDVHKLGGVYKGEFDQLSPKFKGEYQTTENLKLRVGYGRLFKGASLPETLTMKAPSKVKQSDTKAMTGNNYE
AGFDYELTRLLAADYAILGFTAYTYDLDNQMHPTKNNATLSNKYDVEVWGVETVLSYQLDALSVYANHSYSDGEQKSLKN
GKTSHMNKTGIHNFKAGFNYSLNNEFVFGWDSRFVPGNDYENEKGKEIKRSGFATHNIWAAYTPTFAKDLEMNLGIDNLL
DKKYAEHTGFGISWGSEKYTSYEAGRNFKASIAYSF
>Mature_676_residues
MKLKALSVAVTVALTSFTAFAADEPETVVVIGSALDQLIQTEINSDTLEKKQASDIKDVLNTMPSVTVDGNARYSRKVFV
RGMEDKFAVVTIDGARQEGQLFHHSGDQAIDPAMLKRAEIELGGNSALSGSGAINGSFSYETKDPSDLLKPGESVGARVK
TSYQTAYERFATNVAVYAKVNDKLQFMGIANYSEDGDLHIPDQEAVTSKQGELKSGLAKVVFIPNDANEFKLTFNRYSDG
GTRQLSGEKAGALYADDKHDYHSLNRDTVTLQHEYDAGSDLVHLKSNVYYNRQYMDRDALEGTAWGKTPAGKWFKDGKMS
YPKREYNVTTIGGDIRNVSWVGEHELTYGLEGYKAKQWIDAGQGVYLSGTKQGQTENYSMDGGTVTAYGLYLQDMYEFSD
FRLTTGLRYDVHKLGGVYKGEFDQLSPKFKGEYQTTENLKLRVGYGRLFKGASLPETLTMKAPSKVKQSDTKAMTGNNYE
AGFDYELTRLLAADYAILGFTAYTYDLDNQMHPTKNNATLSNKYDVEVWGVETVLSYQLDALSVYANHSYSDGEQKSLKN
GKTSHMNKTGIHNFKAGFNYSLNNEFVFGWDSRFVPGNDYENEKGKEIKRSGFATHNIWAAYTPTFAKDLEMNLGIDNLL
DKKYAEHTGFGISWGSEKYTSYEAGRNFKASIAYSF

Specific function: Heme transporter playing an important role in stationary-phase iron acquisition and required for maintenance of chronic infection in mice [H]

COG id: COG1629

COG function: function code P; Outer membrane receptor proteins, mostly Fe transport

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tonB-dependent receptor family [H]

Homologues:

None

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 75198; Mature: 75198

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00687 ALDEHYDE_DEHYDR_GLU ; PS00430 TONB_DEPENDENT_REC_1 ; PS01156 TONB_DEPENDENT_REC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKALSVAVTVALTSFTAFAADEPETVVVIGSALDQLIQTEINSDTLEKKQASDIKDVL
CCEEEEEEEEEEEEHHHEEECCCCCCEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
NTMPSVTVDGNARYSRKVFVRGMEDKFAVVTIDGARQEGQLFHHSGDQAIDPAMLKRAEI
HHCCCEEECCCCCEEEEEEEEECCCCEEEEEECCCCCCCCEEECCCCCCCCHHHHEEEEE
ELGGNSALSGSGAINGSFSYETKDPSDLLKPGESVGARVKTSYQTAYERFATNVAVYAKV
EECCCCCCCCCCCCCCEEEECCCCHHHHHCCCHHHCCEEEHHHHHHHHHHHCCEEEEEEE
NDKLQFMGIANYSEDGDLHIPDQEAVTSKQGELKSGLAKVVFIPNDANEFKLTFNRYSDG
CCCEEEEEEECCCCCCCEECCCHHHHHCCCCHHHCCCEEEEEECCCCCEEEEEEEEECCC
GTRQLSGEKAGALYADDKHDYHSLNRDTVTLQHEYDAGSDLVHLKSNVYYNRQYMDRDAL
CCEEECCCCCCEEEECCCCHHHCCCCCEEEEEECCCCCCCEEEEECCEEECHHHCCCHHC
EGTAWGKTPAGKWFKDGKMSYPKREYNVTTIGGDIRNVSWVGEHELTYGLEGYKAKQWID
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCEEECCCCCCHHHHHC
AGQGVYLSGTKQGQTENYSMDGGTVTAYGLYLQDMYEFSDFRLTTGLRYDVHKLGGVYKG
CCCCEEEECCCCCCCCCEECCCCCEEEEHHHHHHHHHCCCEEEECCCEEEHHHHCCCCCC
EFDQLSPKFKGEYQTTENLKLRVGYGRLFKGASLPETLTMKAPSKVKQSDTKAMTGNNYE
CHHHCCCCCCCCCCCCCCEEEEECCHHHCCCCCCCCEEEECCCCHHHHCCCCEECCCCCC
AGFDYELTRLLAADYAILGFTAYTYDLDNQMHPTKNNATLSNKYDVEVWGVETVLSYQLD
CCCCHHHHHHHHHCHHEEEEEEEEEECCCCCCCCCCCCEECCCEEEEEEEHHHHHHHCCE
ALSVYANHSYSDGEQKSLKNGKTSHMNKTGIHNFKAGFNYSLNNEFVFGWDSRFVPGNDY
EEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCC
ENEKGKEIKRSGFATHNIWAAYTPTFAKDLEMNLGIDNLLDKKYAEHTGFGISWGSEKYT
CCCCCHHHHHCCCCCCCEEEEECCCHHHCCEECCCHHHHHHHHHHHHCCCCEECCCCCCC
SYEAGRNFKASIAYSF
HHHCCCCEEEEEEECC
>Mature Secondary Structure
MKLKALSVAVTVALTSFTAFAADEPETVVVIGSALDQLIQTEINSDTLEKKQASDIKDVL
CCEEEEEEEEEEEEHHHEEECCCCCCEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
NTMPSVTVDGNARYSRKVFVRGMEDKFAVVTIDGARQEGQLFHHSGDQAIDPAMLKRAEI
HHCCCEEECCCCCEEEEEEEEECCCCEEEEEECCCCCCCCEEECCCCCCCCHHHHEEEEE
ELGGNSALSGSGAINGSFSYETKDPSDLLKPGESVGARVKTSYQTAYERFATNVAVYAKV
EECCCCCCCCCCCCCCEEEECCCCHHHHHCCCHHHCCEEEHHHHHHHHHHHCCEEEEEEE
NDKLQFMGIANYSEDGDLHIPDQEAVTSKQGELKSGLAKVVFIPNDANEFKLTFNRYSDG
CCCEEEEEEECCCCCCCEECCCHHHHHCCCCHHHCCCEEEEEECCCCCEEEEEEEEECCC
GTRQLSGEKAGALYADDKHDYHSLNRDTVTLQHEYDAGSDLVHLKSNVYYNRQYMDRDAL
CCEEECCCCCCEEEECCCCHHHCCCCCEEEEEECCCCCCCEEEEECCEEECHHHCCCHHC
EGTAWGKTPAGKWFKDGKMSYPKREYNVTTIGGDIRNVSWVGEHELTYGLEGYKAKQWID
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCEEECCCCCCHHHHHC
AGQGVYLSGTKQGQTENYSMDGGTVTAYGLYLQDMYEFSDFRLTTGLRYDVHKLGGVYKG
CCCCEEEECCCCCCCCCEECCCCCEEEEHHHHHHHHHCCCEEEECCCEEEHHHHCCCCCC
EFDQLSPKFKGEYQTTENLKLRVGYGRLFKGASLPETLTMKAPSKVKQSDTKAMTGNNYE
CHHHCCCCCCCCCCCCCCEEEEECCHHHCCCCCCCCEEEECCCCHHHHCCCCEECCCCCC
AGFDYELTRLLAADYAILGFTAYTYDLDNQMHPTKNNATLSNKYDVEVWGVETVLSYQLD
CCCCHHHHHHHHHCHHEEEEEEEEEECCCCCCCCCCCCEECCCEEEEEEEHHHHHHHCCE
ALSVYANHSYSDGEQKSLKNGKTSHMNKTGIHNFKAGFNYSLNNEFVFGWDSRFVPGNDY
EEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCC
ENEKGKEIKRSGFATHNIWAAYTPTFAKDLEMNLGIDNLLDKKYAEHTGFGISWGSEKYT
CCCCCHHHHHCCCCCCCEEEEECCCHHHCCEECCCHHHHHHHHHHHHCCCCEECCCCCCC
SYEAGRNFKASIAYSF
HHHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA