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The map label for this gene is tolB

Identifier: 218709110

GI number: 218709110

Start: 1179981

End: 1181333

Strand: Direct

Name: tolB

Synonym: VS_1116

Alternate gene names: 218709110

Gene position: 1179981-1181333 (Clockwise)

Preceding gene: 218709109

Following gene: 218709111

Centisome position: 35.76

GC content: 44.94

Gene sequence:

>1353_bases
GTGTTAAAGAAACTATTACTGAGTTTTGTTTTCTTAATAGCTACAAGCAGCCAGTTTGCCCATGCGGCACTGGAGCTGGT
TATTACAGATGGTATTAACTCTGCACGACCTATCGCTATTGTTCCTTTCCGTTGGGAAGGCGCAGGACCATTGCCGCACG
ATGTGTCAGCAGTTATCGCTTCTGACTTGCAACGCAGTGGTAAATTCAGCCCAGTTGCGACAAGTAAAATGCCTCAAACG
CCTTACAGCGAAACCGAGGTGAATTTTGATGCTTGGACTAACCTAGGTGTTGATTCACTGCTAACAGGTAGCATCACTAA
AAATGCCGAAGGGCAGTATGTGGTGAACTACCAACTGATCGACATTGTTCGTGGTCAACTGACTAAAGGGCAAAGCCGTG
CACTAAGCGATGAAGGCCAATTAGTACTTTCTAAAGATCATGTGCTGTTCAACAAGAAAGCGACAGTGCCTGGTAAACGC
TTGAGAGAGTACGCACACCGAATCTCTGATTTAGTGTATGAAGAGCTAACGGGTGAGAAAGGGGCGTTTCTAACGCGTAT
TTCTTACGTAGTTGTGAACGACAAAGACAAATACCCGTACCAGCTTCGTGTTGCTGACTACGACGGCTTTAACGAGCGTT
TGGTGCTTCGTTCTAAGCAGCCTCTTATGTCACCAGCATGGTCGCCAGACGGTAAGAAGCTTGCGTATGTGAGTTTCCAA
AATGGCCAAGCTGAAATATTCATAATGAATATTTATACGGGTGAGCGCGAGAAAGTGACGTCATACCCTCGTCATAATGG
TGCGCCAAGATTCTCGCCAGATGGTAAAACATTGGCGTTGGTATTGTCGAAAACAGGCAGTCTTCAGGTGTACACACTCG
ACCTTGCTAGCCGTAAGCTGACTCAAATTACCCGCGGTCGTTCTAACAATACTGAACCATTTTGGCACCCAGATGGCAAG
TCTCTAATTTTCACATCTGATCGTGGTGGTAAACCACAGATTTATAATGTAAATTTGTCAAATAATTCGACATCTCGTAT
TACTTGGCAAGGTAGCCAAAACTTAGGTGGCCAGATCACCCCTGACGGACGATTCCTGATTATGGTTAATCGCAGCAACT
CGGGTTTTAACTTGGCTAAACAAGATTTGGAAACTGGTGCGGTTCAGGTGCTAACAAAAACATTGTTAGATGAATCTCCA
AGTATTGCACCGAATGGAGGTATGGTTATCTATAGCTCTATTTACAACAAAACAAACGTACTATCGATGGTTTCTATCGA
TGGTCGTTTTAAGGCTAGATTACCAGCAACGAACGGACGCGTAAGAGCGCCTTCGTGGTCACCGTTTCTCTAG

Upstream 100 bases:

>100_bases
AGCACAAGTGCAATCTTACCCGTTACCTAAGGACCCAGACATTATAAAATCACTGAAAGACATTAACTTAACTGTATCAC
CAGAGTAAAAGGACGAACTT

Downstream 100 bases:

>100_bases
TCGATTTGATACATTCTTTTAGCTATTGATAGTGTTAACTATTGTGGCATTTAACTATCTATAGCACTTAGTCGCTGTAG
AAACTAAGTTTGATAACGTA

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MLKKLLLSFVFLIATSSQFAHAALELVITDGINSARPIAIVPFRWEGAGPLPHDVSAVIASDLQRSGKFSPVATSKMPQT
PYSETEVNFDAWTNLGVDSLLTGSITKNAEGQYVVNYQLIDIVRGQLTKGQSRALSDEGQLVLSKDHVLFNKKATVPGKR
LREYAHRISDLVYEELTGEKGAFLTRISYVVVNDKDKYPYQLRVADYDGFNERLVLRSKQPLMSPAWSPDGKKLAYVSFQ
NGQAEIFIMNIYTGEREKVTSYPRHNGAPRFSPDGKTLALVLSKTGSLQVYTLDLASRKLTQITRGRSNNTEPFWHPDGK
SLIFTSDRGGKPQIYNVNLSNNSTSRITWQGSQNLGGQITPDGRFLIMVNRSNSGFNLAKQDLETGAVQVLTKTLLDESP
SIAPNGGMVIYSSIYNKTNVLSMVSIDGRFKARLPATNGRVRAPSWSPFL

Sequences:

>Translated_450_residues
MLKKLLLSFVFLIATSSQFAHAALELVITDGINSARPIAIVPFRWEGAGPLPHDVSAVIASDLQRSGKFSPVATSKMPQT
PYSETEVNFDAWTNLGVDSLLTGSITKNAEGQYVVNYQLIDIVRGQLTKGQSRALSDEGQLVLSKDHVLFNKKATVPGKR
LREYAHRISDLVYEELTGEKGAFLTRISYVVVNDKDKYPYQLRVADYDGFNERLVLRSKQPLMSPAWSPDGKKLAYVSFQ
NGQAEIFIMNIYTGEREKVTSYPRHNGAPRFSPDGKTLALVLSKTGSLQVYTLDLASRKLTQITRGRSNNTEPFWHPDGK
SLIFTSDRGGKPQIYNVNLSNNSTSRITWQGSQNLGGQITPDGRFLIMVNRSNSGFNLAKQDLETGAVQVLTKTLLDESP
SIAPNGGMVIYSSIYNKTNVLSMVSIDGRFKARLPATNGRVRAPSWSPFL
>Mature_450_residues
MLKKLLLSFVFLIATSSQFAHAALELVITDGINSARPIAIVPFRWEGAGPLPHDVSAVIASDLQRSGKFSPVATSKMPQT
PYSETEVNFDAWTNLGVDSLLTGSITKNAEGQYVVNYQLIDIVRGQLTKGQSRALSDEGQLVLSKDHVLFNKKATVPGKR
LREYAHRISDLVYEELTGEKGAFLTRISYVVVNDKDKYPYQLRVADYDGFNERLVLRSKQPLMSPAWSPDGKKLAYVSFQ
NGQAEIFIMNIYTGEREKVTSYPRHNGAPRFSPDGKTLALVLSKTGSLQVYTLDLASRKLTQITRGRSNNTEPFWHPDGK
SLIFTSDRGGKPQIYNVNLSNNSTSRITWQGSQNLGGQITPDGRFLIMVNRSNSGFNLAKQDLETGAVQVLTKTLLDESP
SIAPNGGMVIYSSIYNKTNVLSMVSIDGRFKARLPATNGRVRAPSWSPFL

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=450, Percent_Identity=50.2222222222222, Blast_Score=466, Evalue=1e-132,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_VIBSL (B7VMJ2)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002416731.1
- ProteinModelPortal:   B7VMJ2
- SMR:   B7VMJ2
- GeneID:   7160708
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_1116
- HOGENOM:   HBG644919
- OMA:   RVADYDG
- ProtClustDB:   PRK04792
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- Gene3D:   G3DSA:3.40.50.10070
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 49774; Mature: 49774

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKLLLSFVFLIATSSQFAHAALELVITDGINSARPIAIVPFRWEGAGPLPHDVSAVIA
CHHHHHHHHHHHHHCCCHHHHEEEEEEEECCCCCCCCEEEEEEEECCCCCCCHHHHHHHH
SDLQRSGKFSPVATSKMPQTPYSETEVNFDAWTNLGVDSLLTGSITKNAEGQYVVNYQLI
HHHHHCCCCCCCCCCCCCCCCCCCCEECHHHHCCCCHHHHEECCCCCCCCCCEEEEEEEE
DIVRGQLTKGQSRALSDEGQLVLSKDHVLFNKKATVPGKRLREYAHRISDLVYEELTGEK
EEHHCCCCCCCCCCCCCCCCEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC
GAFLTRISYVVVNDKDKYPYQLRVADYDGFNERLVLRSKQPLMSPAWSPDGKKLAYVSFQ
CCEEEEEEEEEEECCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEEC
NGQAEIFIMNIYTGEREKVTSYPRHNGAPRFSPDGKTLALVLSKTGSLQVYTLDLASRKL
CCCEEEEEEEEEECCCHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCHHHH
TQITRGRSNNTEPFWHPDGKSLIFTSDRGGKPQIYNVNLSNNSTSRITWQGSQNLGGQIT
HHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCEEEEECCCCCCCCEEC
PDGRFLIMVNRSNSGFNLAKQDLETGAVQVLTKTLLDESPSIAPNGGMVIYSSIYNKTNV
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCE
LSMVSIDGRFKARLPATNGRVRAPSWSPFL
EEEEEECCEEEEECCCCCCEEECCCCCCCC
>Mature Secondary Structure
MLKKLLLSFVFLIATSSQFAHAALELVITDGINSARPIAIVPFRWEGAGPLPHDVSAVIA
CHHHHHHHHHHHHHCCCHHHHEEEEEEEECCCCCCCCEEEEEEEECCCCCCCHHHHHHHH
SDLQRSGKFSPVATSKMPQTPYSETEVNFDAWTNLGVDSLLTGSITKNAEGQYVVNYQLI
HHHHHCCCCCCCCCCCCCCCCCCCCEECHHHHCCCCHHHHEECCCCCCCCCCEEEEEEEE
DIVRGQLTKGQSRALSDEGQLVLSKDHVLFNKKATVPGKRLREYAHRISDLVYEELTGEK
EEHHCCCCCCCCCCCCCCCCEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC
GAFLTRISYVVVNDKDKYPYQLRVADYDGFNERLVLRSKQPLMSPAWSPDGKKLAYVSFQ
CCEEEEEEEEEEECCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEEC
NGQAEIFIMNIYTGEREKVTSYPRHNGAPRFSPDGKTLALVLSKTGSLQVYTLDLASRKL
CCCEEEEEEEEEECCCHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCHHHH
TQITRGRSNNTEPFWHPDGKSLIFTSDRGGKPQIYNVNLSNNSTSRITWQGSQNLGGQIT
HHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCEEEEECCCCCCCCEEC
PDGRFLIMVNRSNSGFNLAKQDLETGAVQVLTKTLLDESPSIAPNGGMVIYSSIYNKTNV
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCE
LSMVSIDGRFKARLPATNGRVRAPSWSPFL
EEEEEECCEEEEECCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA