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The map label for this gene is fliF [H]

Identifier: 218708823

GI number: 218708823

Start: 844392

End: 846134

Strand: Direct

Name: fliF [H]

Synonym: VS_0823

Alternate gene names: 218708823

Gene position: 844392-846134 (Clockwise)

Preceding gene: 218708822

Following gene: 218708824

Centisome position: 25.59

GC content: 45.44

Gene sequence:

>1743_bases
GTGGCAGATAACAGTCAAACAACAGATTTAGCCGTAAGCGATAGCAACGACCACGCACTTATTGCAAGTTCTGATCTCGA
TGGCGAAGGGCAAAATACCGATTTAGGTGAGCGCAGTTCATCTAAGTTCGATATGGCCGTCGGTGATCTCGATTTGCTTC
GTCAAGTCGTCTTAGTTCTTTCTATCTCGATCTGTGTAGCGCTTATTGTAATGCTGTTTTTCTGGGTGAAAGAGCCAGAA
ATGCGTCCATTAGGGGCTTACGAAACAGAAGAGTTGATCCCGGTTCTTGATTATTTGGATACGCAGAAGATTGAGTACTC
TCTTGAAGGGAATACCATCTCGGTACCAGCGAGTGAATATAACTCATTAAAGCTAAATATGGTTCGAGCGGGTTTGAACC
AAGAGCGAAACACCGGTGATGATATCCTCATGCAAGACATGGGTTTTGGTGTATCACAACGTTTAGAGAAAGAGCGTCTT
AAACTAAGTCGTGAAAGACAGCTTGCTAAAGCTATTGAACAGATGAAGCAGGTACGTAAAGCTCAAGTTTTACTTGCCTT
ACCTAAGCAAAGTGTCTTTGTTCGTCACAATCAAGAAGCCTCTGCCTCAGTATTCCTGACACTTAAAACGGGGACGAACC
TCAAGCAGCAAGAAGTTGATTCTGTTGTGGACATGGTAGCCAGTGCCGTTCCGGGGATGAAAACTTCGCGAATTACGGTG
ACCGACCAGCATGGCCGACTTTTGAGCTCTGGCTCTCAGGATCCGATGTCAGCGGCACGCCGAAAAGAGCACGAGTTAGA
GCGTAATCAAGAACAAGCGCTACGTGAAAAAATTGACTCTATTTTGATTCCGATTCTTGGCTTTGGTAACTACACGGCTC
AGGTTGATATTCAACTCGACTTTAGTGCTGTGGAACAAACAAGAAAGCGTTTTGATCCGAATACTCCAGCAACCCGAAGT
GAATACACATTAGAAGACTATAATAACGGCAACGTTGTGGCTGGTATTCCAGGGGCTTTGAGTAACCAGCCTCCTGCTGA
TGCTTCGATTCCTCAGGATGTGGCTCAGATGAAAGATGGTACGATGATGGGGCAAGGTTCGGTTCACAAAGAAGCGACCC
GCAACTTTGAGCTGGATACTACCATTAGTCATGAGCGAAAACAGAGCGGCACGGTTAATCGTCAGACCGTATCTGTCGCA
ATCAAAGATCGCCAATCATTGAACCCTGATACGGGAGAAGTGGTTCACACCCCAATTCCAGCAAGCGAAATTAACGCGAT
ACGTCAGGTATTGATTGGAACTGTGGGTTTTGATGAAAACCGCGGTGATTTACTCAATGTGTTAAGTATGCAATTTGCAC
CTCAAGTAACGGATATTGTTGCTGACGTACCAATTTGGGAGCATCAACACTTTAATGATTGGGTTCGTTGGTTTGCGAGT
GCGTTGGTTATTATTGTTGTCGTATTGGTGCTGGTTCGCCCTGCAATGAAAAAACTGCTTAACCCAGCAGCAGACGATGA
CGATCAAATGTACGGCCCCGATGGCATGCCAATTGGTGCTGACGGCGAAACCAGTTTAATTGGTGGTGATATTGAAGGTG
GTGAGTTATTTGAATTTGGTTCAAGTATCGACCTACCTAACCTTCACAAAGATGAGGACGTACTGAAAGCAGTACGTGCA
CTTGTAGCGAATGAACCAGAGCTAGCAGCTCAAGTAGTTAAGAATTGGGTGGCAGATGGCTAA

Upstream 100 bases:

>100_bases
ATCTAGTGTGGCTTTTGAAGCGACGATCCAAATTAGAAATAAATTGGTCGAGTCGTACAAAGAGCTGATGAACATGCCGG
TATAAGTTGGGTAGTAAATA

Downstream 100 bases:

>100_bases
CGAAATTGTTCCACAACAAGCAGAAGGTGGTGAGCTTGATGTCGCAAGTGTCGATATCGAGTCTATCTCAGGCGAAGAGC
GCGCTGCGATCTTGTTGCTA

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 580; Mature: 579

Protein sequence:

>580_residues
MADNSQTTDLAVSDSNDHALIASSDLDGEGQNTDLGERSSSKFDMAVGDLDLLRQVVLVLSISICVALIVMLFFWVKEPE
MRPLGAYETEELIPVLDYLDTQKIEYSLEGNTISVPASEYNSLKLNMVRAGLNQERNTGDDILMQDMGFGVSQRLEKERL
KLSRERQLAKAIEQMKQVRKAQVLLALPKQSVFVRHNQEASASVFLTLKTGTNLKQQEVDSVVDMVASAVPGMKTSRITV
TDQHGRLLSSGSQDPMSAARRKEHELERNQEQALREKIDSILIPILGFGNYTAQVDIQLDFSAVEQTRKRFDPNTPATRS
EYTLEDYNNGNVVAGIPGALSNQPPADASIPQDVAQMKDGTMMGQGSVHKEATRNFELDTTISHERKQSGTVNRQTVSVA
IKDRQSLNPDTGEVVHTPIPASEINAIRQVLIGTVGFDENRGDLLNVLSMQFAPQVTDIVADVPIWEHQHFNDWVRWFAS
ALVIIVVVLVLVRPAMKKLLNPAADDDDQMYGPDGMPIGADGETSLIGGDIEGGELFEFGSSIDLPNLHKDEDVLKAVRA
LVANEPELAAQVVKNWVADG

Sequences:

>Translated_580_residues
MADNSQTTDLAVSDSNDHALIASSDLDGEGQNTDLGERSSSKFDMAVGDLDLLRQVVLVLSISICVALIVMLFFWVKEPE
MRPLGAYETEELIPVLDYLDTQKIEYSLEGNTISVPASEYNSLKLNMVRAGLNQERNTGDDILMQDMGFGVSQRLEKERL
KLSRERQLAKAIEQMKQVRKAQVLLALPKQSVFVRHNQEASASVFLTLKTGTNLKQQEVDSVVDMVASAVPGMKTSRITV
TDQHGRLLSSGSQDPMSAARRKEHELERNQEQALREKIDSILIPILGFGNYTAQVDIQLDFSAVEQTRKRFDPNTPATRS
EYTLEDYNNGNVVAGIPGALSNQPPADASIPQDVAQMKDGTMMGQGSVHKEATRNFELDTTISHERKQSGTVNRQTVSVA
IKDRQSLNPDTGEVVHTPIPASEINAIRQVLIGTVGFDENRGDLLNVLSMQFAPQVTDIVADVPIWEHQHFNDWVRWFAS
ALVIIVVVLVLVRPAMKKLLNPAADDDDQMYGPDGMPIGADGETSLIGGDIEGGELFEFGSSIDLPNLHKDEDVLKAVRA
LVANEPELAAQVVKNWVADG
>Mature_579_residues
ADNSQTTDLAVSDSNDHALIASSDLDGEGQNTDLGERSSSKFDMAVGDLDLLRQVVLVLSISICVALIVMLFFWVKEPEM
RPLGAYETEELIPVLDYLDTQKIEYSLEGNTISVPASEYNSLKLNMVRAGLNQERNTGDDILMQDMGFGVSQRLEKERLK
LSRERQLAKAIEQMKQVRKAQVLLALPKQSVFVRHNQEASASVFLTLKTGTNLKQQEVDSVVDMVASAVPGMKTSRITVT
DQHGRLLSSGSQDPMSAARRKEHELERNQEQALREKIDSILIPILGFGNYTAQVDIQLDFSAVEQTRKRFDPNTPATRSE
YTLEDYNNGNVVAGIPGALSNQPPADASIPQDVAQMKDGTMMGQGSVHKEATRNFELDTTISHERKQSGTVNRQTVSVAI
KDRQSLNPDTGEVVHTPIPASEINAIRQVLIGTVGFDENRGDLLNVLSMQFAPQVTDIVADVPIWEHQHFNDWVRWFASA
LVIIVVVLVLVRPAMKKLLNPAADDDDQMYGPDGMPIGADGETSLIGGDIEGGELFEFGSSIDLPNLHKDEDVLKAVRAL
VANEPELAAQVVKNWVADG

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=448, Percent_Identity=32.5892857142857, Blast_Score=186, Evalue=4e-48,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 63799; Mature: 63668

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADNSQTTDLAVSDSNDHALIASSDLDGEGQNTDLGERSSSKFDMAVGDLDLLRQVVLVL
CCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHH
SISICVALIVMLFFWVKEPEMRPLGAYETEELIPVLDYLDTQKIEYSLEGNTISVPASEY
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCEEECCCCCC
NSLKLNMVRAGLNQERNTGDDILMQDMGFGVSQRLEKERLKLSRERQLAKAIEQMKQVRK
CCEEEEHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQVLLALPKQSVFVRHNQEASASVFLTLKTGTNLKQQEVDSVVDMVASAVPGMKTSRITV
HHHHHCCCCCCEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
TDQHGRLLSSGSQDPMSAARRKEHELERNQEQALREKIDSILIPILGFGNYTAQVDIQLD
ECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEC
FSAVEQTRKRFDPNTPATRSEYTLEDYNNGNVVAGIPGALSNQPPADASIPQDVAQMKDG
HHHHHHHHHHCCCCCCCCCCCCEEEECCCCCEEEECCCCCCCCCCCCCCCHHHHHHHCCC
TMMGQGSVHKEATRNFELDTTISHERKQSGTVNRQTVSVAIKDRQSLNPDTGEVVHTPIP
CEECCCCCCHHHHCCCCCCCCCCHHHHHCCCCCCEEEEEEEECCCCCCCCCCCEEECCCC
ASEINAIRQVLIGTVGFDENRGDLLNVLSMQFAPQVTDIVADVPIWEHQHFNDWVRWFAS
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHH
ALVIIVVVLVLVRPAMKKLLNPAADDDDQMYGPDGMPIGADGETSLIGGDIEGGELFEFG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCHHHHCC
SSIDLPNLHKDEDVLKAVRALVANEPELAAQVVKNWVADG
CCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ADNSQTTDLAVSDSNDHALIASSDLDGEGQNTDLGERSSSKFDMAVGDLDLLRQVVLVL
CCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHH
SISICVALIVMLFFWVKEPEMRPLGAYETEELIPVLDYLDTQKIEYSLEGNTISVPASEY
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCEEECCCCCC
NSLKLNMVRAGLNQERNTGDDILMQDMGFGVSQRLEKERLKLSRERQLAKAIEQMKQVRK
CCEEEEHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQVLLALPKQSVFVRHNQEASASVFLTLKTGTNLKQQEVDSVVDMVASAVPGMKTSRITV
HHHHHCCCCCCEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
TDQHGRLLSSGSQDPMSAARRKEHELERNQEQALREKIDSILIPILGFGNYTAQVDIQLD
ECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEC
FSAVEQTRKRFDPNTPATRSEYTLEDYNNGNVVAGIPGALSNQPPADASIPQDVAQMKDG
HHHHHHHHHHCCCCCCCCCCCCEEEECCCCCEEEECCCCCCCCCCCCCCCHHHHHHHCCC
TMMGQGSVHKEATRNFELDTTISHERKQSGTVNRQTVSVAIKDRQSLNPDTGEVVHTPIP
CEECCCCCCHHHHCCCCCCCCCCHHHHHCCCCCCEEEEEEEECCCCCCCCCCCEEECCCC
ASEINAIRQVLIGTVGFDENRGDLLNVLSMQFAPQVTDIVADVPIWEHQHFNDWVRWFAS
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHH
ALVIIVVVLVLVRPAMKKLLNPAADDDDQMYGPDGMPIGADGETSLIGGDIEGGELFEFG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCHHHHCC
SSIDLPNLHKDEDVLKAVRALVANEPELAAQVVKNWVADG
CCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8675317; 10984043 [H]