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The map label for this gene is holA [H]

Identifier: 218708737

GI number: 218708737

Start: 750705

End: 751730

Strand: Reverse

Name: holA [H]

Synonym: VS_0716

Alternate gene names: 218708737

Gene position: 751730-750705 (Counterclockwise)

Preceding gene: 218708738

Following gene: 218708736

Centisome position: 22.78

GC content: 45.03

Gene sequence:

>1026_bases
ATGCGTATTTTTGCTGATCGTTTATCTGAGCAACTTGCTAAGCAGTTAAGTAACGTTTACCTAATTTTTGGTAACGAACC
TTTACTGCTGCAGGAAAGCCGAGAAGCGATTCAAAAAACAGCCAAAGAGCAAGGCTTCGAAGAACGCCACCGCTTTGCTA
TTGATAGCAGTCTGGACTGGAATCAAGTCTACGACTGCACTCAAGCATTGAGCTTATTCTCTAGTCGTCAGCTCATCGAA
CTTGAATTGCCAGAGTCCGGGGTCAATGCCGCAATAGCAAAAGAGCTGCTCGCAATTTCAGAGCATATTCATAGCGACAT
CTTGTTGATCTTGGTGGGTACCAAGCTCACTAAAGCTCAAGAAAGCGCTAAGTGGTTTAAAGCACTCTCGAATCAAGGCC
ACTGGATCAGCTGCCTAACCCCAGACGTTAGCCGATTACCTCAGTTTGTTCAAACACGCTGTCGTCAGATCGGCTTATCA
CCGGATCCTGAAGCGATACAAATGTTGGCGCAATGGCATGAGGGTAATCTATTTGCGCTCACCCAAAGCCTAGAAAAACT
GGCTCTCCAATACCCAGATGGAAAGCTCACATTAGTACGTTTAGAAGAATCGCTGAGTCGACACAACCACTTTACGCCAT
TCCACTGGAGTGATGCGTTATTAGCAGGCAAAGGCAATCGTGCACAGCGAATCCTGCGTCAGTTAGAAGCCGAAGGTGTC
GAACCCGTTATCTTATTGCGCAGCATACAACGTGAGCTTGCTCTGCTATTACAAATGCAGCAACAAATGAAGCAAATGCC
AATCGCGCAGGTATTTGAAAAGCACCGTATCTGGCAATCCAAAAAACCTCTTTATAACTCTGCGCTAACAAGAGTTTCGA
TATCTCAATTACACTCCCTGTTTGCGCTACTCACTCAAGTAGAATTGATGATAAAAACGCAATATGAACAGTCGCCTTGG
CCACTAATTCATCAGTTAAGCGTAGAGTTTTGTATCCCTTCAGCTTCAATCCCATTTCACGCATAA

Upstream 100 bases:

>100_bases
GTACAACATCAAAAACATCGAAATTGACGATGTTGAGGTCGACGCAAGTGCAGTACCTGATGCTGAGCCTAGCGTAACTG
TCGAGCAATAGGTTTATTTA

Downstream 100 bases:

>100_bases
AAGCGTGGTATATTCTAGGGTCAACAGACCCTAAAAAGCTAAACTCAGATTAAAGGAACACCCCGTGTTACGTGAAGAAC
TAAAAGATTTTCTTGCAGAC

Product: DNA polymerase III subunit delta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MRIFADRLSEQLAKQLSNVYLIFGNEPLLLQESREAIQKTAKEQGFEERHRFAIDSSLDWNQVYDCTQALSLFSSRQLIE
LELPESGVNAAIAKELLAISEHIHSDILLILVGTKLTKAQESAKWFKALSNQGHWISCLTPDVSRLPQFVQTRCRQIGLS
PDPEAIQMLAQWHEGNLFALTQSLEKLALQYPDGKLTLVRLEESLSRHNHFTPFHWSDALLAGKGNRAQRILRQLEAEGV
EPVILLRSIQRELALLLQMQQQMKQMPIAQVFEKHRIWQSKKPLYNSALTRVSISQLHSLFALLTQVELMIKTQYEQSPW
PLIHQLSVEFCIPSASIPFHA

Sequences:

>Translated_341_residues
MRIFADRLSEQLAKQLSNVYLIFGNEPLLLQESREAIQKTAKEQGFEERHRFAIDSSLDWNQVYDCTQALSLFSSRQLIE
LELPESGVNAAIAKELLAISEHIHSDILLILVGTKLTKAQESAKWFKALSNQGHWISCLTPDVSRLPQFVQTRCRQIGLS
PDPEAIQMLAQWHEGNLFALTQSLEKLALQYPDGKLTLVRLEESLSRHNHFTPFHWSDALLAGKGNRAQRILRQLEAEGV
EPVILLRSIQRELALLLQMQQQMKQMPIAQVFEKHRIWQSKKPLYNSALTRVSISQLHSLFALLTQVELMIKTQYEQSPW
PLIHQLSVEFCIPSASIPFHA
>Mature_341_residues
MRIFADRLSEQLAKQLSNVYLIFGNEPLLLQESREAIQKTAKEQGFEERHRFAIDSSLDWNQVYDCTQALSLFSSRQLIE
LELPESGVNAAIAKELLAISEHIHSDILLILVGTKLTKAQESAKWFKALSNQGHWISCLTPDVSRLPQFVQTRCRQIGLS
PDPEAIQMLAQWHEGNLFALTQSLEKLALQYPDGKLTLVRLEESLSRHNHFTPFHWSDALLAGKGNRAQRILRQLEAEGV
EPVILLRSIQRELALLLQMQQQMKQMPIAQVFEKHRIWQSKKPLYNSALTRVSISQLHSLFALLTQVELMIKTQYEQSPW
PLIHQLSVEFCIPSASIPFHA

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The delta subunit seems to interact with the gamma subunit to transfer the b

COG id: COG1466

COG function: function code L; DNA polymerase III, delta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786859, Length=331, Percent_Identity=48.6404833836858, Blast_Score=309, Evalue=1e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR010372
- InterPro:   IPR005790 [H]

Pfam domain/function: PF06144 DNA_pol3_delta [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 39031; Mature: 39031

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIFADRLSEQLAKQLSNVYLIFGNEPLLLQESREAIQKTAKEQGFEERHRFAIDSSLDW
CCHHHHHHHHHHHHHHCCEEEEECCCCEEHHHHHHHHHHHHHHCCHHHHHHECCCCCCCH
NQVYDCTQALSLFSSRQLIELELPESGVNAAIAKELLAISEHIHSDILLILVGTKLTKAQ
HHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHH
ESAKWFKALSNQGHWISCLTPDVSRLPQFVQTRCRQIGLSPDPEAIQMLAQWHEGNLFAL
HHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHH
TQSLEKLALQYPDGKLTLVRLEESLSRHNHFTPFHWSDALLAGKGNRAQRILRQLEAEGV
HHHHHHHHHCCCCCCEEEEEEHHHHHHCCCCCCCCCCCHHEECCCHHHHHHHHHHHHCCC
EPVILLRSIQRELALLLQMQQQMKQMPIAQVFEKHRIWQSKKPLYNSALTRVSISQLHSL
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
FALLTQVELMIKTQYEQSPWPLIHQLSVEFCIPSASIPFHA
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MRIFADRLSEQLAKQLSNVYLIFGNEPLLLQESREAIQKTAKEQGFEERHRFAIDSSLDW
CCHHHHHHHHHHHHHHCCEEEEECCCCEEHHHHHHHHHHHHHHCCHHHHHHECCCCCCCH
NQVYDCTQALSLFSSRQLIELELPESGVNAAIAKELLAISEHIHSDILLILVGTKLTKAQ
HHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHH
ESAKWFKALSNQGHWISCLTPDVSRLPQFVQTRCRQIGLSPDPEAIQMLAQWHEGNLFAL
HHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHH
TQSLEKLALQYPDGKLTLVRLEESLSRHNHFTPFHWSDALLAGKGNRAQRILRQLEAEGV
HHHHHHHHHCCCCCCEEEEEEHHHHHHCCCCCCCCCCCHHEECCCHHHHHHHHHHHHCCC
EPVILLRSIQRELALLLQMQQQMKQMPIAQVFEKHRIWQSKKPLYNSALTRVSISQLHSL
CHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
FALLTQVELMIKTQYEQSPWPLIHQLSVEFCIPSASIPFHA
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8505303; 1400251; 8905232; 9278503; 3316191; 8505304; 1575709 [H]