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The map label for this gene is apbE [H]

Identifier: 218708720

GI number: 218708720

Start: 733138

End: 734142

Strand: Direct

Name: apbE [H]

Synonym: VS_0699

Alternate gene names: 218708720

Gene position: 733138-734142 (Clockwise)

Preceding gene: 218708719

Following gene: 218708721

Centisome position: 22.22

GC content: 44.38

Gene sequence:

>1005_bases
GTGAGAATTTGGCTTGTTGCATTAACTTCTTTGATTTTTCTTGCGGGCTGTGAGCAGGCAAGAGAGCAAGTACATTTAAG
TGGTCCGACAATGGGGACCAGTTATAACATTAAGTACATCAATGGCGATGAATTTCCTGAGTCTAAAGAAGTTCATACCG
AGATCGATCGTCTACTTGAAGAAGTGAATGATCAAATGTCGACTTACCGTGAAGATTCAGAGCTGAGCCGTTTCAACCAA
CACAAAGGTGCGGACGCATTCGAAGTATCTGAACAAACCGCTATCGTTGTGAAAGAAGCGATTCGTTTGAACGGTCTTAC
TGAAGGTGCGTTGGATGTGACGGTTGGTCCATTAGTTAACTTGTGGGGTTTTGGTCCAGAAGCACGTCCTGAAGTGGTTC
CATCAGACGAAGAGCTTGCGGCTCGTAAGGCTAAGATTGGTATTCACCACTTAAGCGTTGAAGGTAATAAGCTAAGCAAA
GATTTGCCTAACTTGTATGTTGACCTTTCGACTATCGCAAAAGGTTGGGGCGTGGATGTCGTTGCTGACTACCTCGATTC
AATTGGCATTCATAACTACATGGTAGAAGTGGGCGGTGAAATCCGTTTGAAGGGCTTAAACCGTGAAAGTGTGGGTTGGC
GTATCGCCATCGAGAAGCCAAGTGTTGACGAGCGCAACATTCAAGAGATCATCGAGCCTGGTGATATGGCGATCGCAACG
TCTGGCGACTACCGAAACTATTTTGAGCGTGATGGTGTTCGTTATTCACACATCATCAACCCAGAAACAGGTAAGCCACT
TCATCACAAAGTGGTTTCAGTGACTGTTTTAAACCCGTCTTCAATGACTGCAGACGGTTTATCTACTGGCCTTATGGTCT
TAGGTGAAGAAAAAGGACTGGAAGTCGCAAACCAACATAACATCCCAGTGTTCATGGTGGTAAAAACAGCTGATGGCTTT
AAAGAAATTGCTTCAGAAGCATTTAAGCCATACTTAGGTAAATAA

Upstream 100 bases:

>100_bases
CAAACTGGCTTATATAAGAAAGAGTAAGGCATTTAAAATACCTATTTTTCACTCTTATTTTTCCTTATATAAATCCTCAA
CATTAGACAGGAGTAAGCAA

Downstream 100 bases:

>100_bases
GGTAAGCGAGATAATTATGAATACATTTCTGATTACATTTGGTGTTTTTCTTGCAGTAATCGCAGCGATGTCAATTGGCT
ATATTATCCAAAAGAAAGTT

Product: thiamine biosynthesis lipoprotein ApbE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MRIWLVALTSLIFLAGCEQAREQVHLSGPTMGTSYNIKYINGDEFPESKEVHTEIDRLLEEVNDQMSTYREDSELSRFNQ
HKGADAFEVSEQTAIVVKEAIRLNGLTEGALDVTVGPLVNLWGFGPEARPEVVPSDEELAARKAKIGIHHLSVEGNKLSK
DLPNLYVDLSTIAKGWGVDVVADYLDSIGIHNYMVEVGGEIRLKGLNRESVGWRIAIEKPSVDERNIQEIIEPGDMAIAT
SGDYRNYFERDGVRYSHIINPETGKPLHHKVVSVTVLNPSSMTADGLSTGLMVLGEEKGLEVANQHNIPVFMVVKTADGF
KEIASEAFKPYLGK

Sequences:

>Translated_334_residues
MRIWLVALTSLIFLAGCEQAREQVHLSGPTMGTSYNIKYINGDEFPESKEVHTEIDRLLEEVNDQMSTYREDSELSRFNQ
HKGADAFEVSEQTAIVVKEAIRLNGLTEGALDVTVGPLVNLWGFGPEARPEVVPSDEELAARKAKIGIHHLSVEGNKLSK
DLPNLYVDLSTIAKGWGVDVVADYLDSIGIHNYMVEVGGEIRLKGLNRESVGWRIAIEKPSVDERNIQEIIEPGDMAIAT
SGDYRNYFERDGVRYSHIINPETGKPLHHKVVSVTVLNPSSMTADGLSTGLMVLGEEKGLEVANQHNIPVFMVVKTADGF
KEIASEAFKPYLGK
>Mature_334_residues
MRIWLVALTSLIFLAGCEQAREQVHLSGPTMGTSYNIKYINGDEFPESKEVHTEIDRLLEEVNDQMSTYREDSELSRFNQ
HKGADAFEVSEQTAIVVKEAIRLNGLTEGALDVTVGPLVNLWGFGPEARPEVVPSDEELAARKAKIGIHHLSVEGNKLSK
DLPNLYVDLSTIAKGWGVDVVADYLDSIGIHNYMVEVGGEIRLKGLNRESVGWRIAIEKPSVDERNIQEIIEPGDMAIAT
SGDYRNYFERDGVRYSHIINPETGKPLHHKVVSVTVLNPSSMTADGLSTGLMVLGEEKGLEVANQHNIPVFMVVKTADGF
KEIASEAFKPYLGK

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=337, Percent_Identity=43.3234421364985, Blast_Score=288, Evalue=3e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 36916; Mature: 36916

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIWLVALTSLIFLAGCEQAREQVHLSGPTMGTSYNIKYINGDEFPESKEVHTEIDRLLE
CEEHHHHHHHHHHHHCCHHHHHEEEECCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHH
EVNDQMSTYREDSELSRFNQHKGADAFEVSEQTAIVVKEAIRLNGLTEGALDVTVGPLVN
HHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEHHHHHH
LWGFGPEARPEVVPSDEELAARKAKIGIHHLSVEGNKLSKDLPNLYVDLSTIAKGWGVDV
HHCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEECCCHHHHCCCHHEEEHHHHHCCCCHHH
VADYLDSIGIHNYMVEVGGEIRLKGLNRESVGWRIAIEKPSVDERNIQEIIEPGDMAIAT
HHHHHHHCCHHHEEEEECCEEEEEECCCCCCCEEEEEECCCCCHHHHHHHCCCCCEEEEE
SGDYRNYFERDGVRYSHIINPETGKPLHHKVVSVTVLNPSSMTADGLSTGLMVLGEEKGL
CCCHHHHHHHCCCEEEEEECCCCCCCCEEEEEEEEEECCCCCCCCCCCCCEEEEECCCCC
EVANQHNIPVFMVVKTADGFKEIASEAFKPYLGK
CCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRIWLVALTSLIFLAGCEQAREQVHLSGPTMGTSYNIKYINGDEFPESKEVHTEIDRLLE
CEEHHHHHHHHHHHHCCHHHHHEEEECCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHH
EVNDQMSTYREDSELSRFNQHKGADAFEVSEQTAIVVKEAIRLNGLTEGALDVTVGPLVN
HHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEHHHHHH
LWGFGPEARPEVVPSDEELAARKAKIGIHHLSVEGNKLSKDLPNLYVDLSTIAKGWGVDV
HHCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEECCCHHHHCCCHHEEEHHHHHCCCCHHH
VADYLDSIGIHNYMVEVGGEIRLKGLNRESVGWRIAIEKPSVDERNIQEIIEPGDMAIAT
HHHHHHHCCHHHEEEEECCEEEEEECCCCCCCEEEEEECCCCCHHHHHHHCCCCCEEEEE
SGDYRNYFERDGVRYSHIINPETGKPLHHKVVSVTVLNPSSMTADGLSTGLMVLGEEKGL
CCCHHHHHHHCCCEEEEEECCCCCCCCEEEEEEEEEECCCCCCCCCCCCCEEEEECCCCC
EVANQHNIPVFMVVKTADGFKEIASEAFKPYLGK
CCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]