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The map label for this gene is yeiE [H]

Identifier: 218708692

GI number: 218708692

Start: 699755

End: 700651

Strand: Reverse

Name: yeiE [H]

Synonym: VS_0671

Alternate gene names: 218708692

Gene position: 700651-699755 (Counterclockwise)

Preceding gene: 218708698

Following gene: 218708691

Centisome position: 21.24

GC content: 44.48

Gene sequence:

>897_bases
ATGGCTAATATTTCAATCAAACAACTCAAAGTGTTTGTCACTATTACCCAGCATTCGACGCTTACCGCTGCATCAGAAGC
GCTATTTCTGTCTAAAGCAGCTGTCAGCATGGCGCTAGGAGAAATGGAGAAACAGTTAGGTCACTCTCTATTTGACCGGG
TCAACAACCGATTAATTCTCAATCAAGAAGGACAAAAGCTACTTCCTTTGGCTGACGAGATTCTTCATAGAGCTGCAGGT
ATCGATGTTTTGTTCCGCGACGACCAACCGTTAAGTGGTAATCTAAAGGTTGGCGCGAGTGACACGATTGGTAATCAAGT
CGCACCGTTTATTCTGTCTGGCTTTCGCGAGCTAACTCAGCATCAAGATCAGAGCTTATTCATTTCAAACAGTGCGCTGA
TCTGCCAAAAGTTGGTGGATTATGAACTGGATATCGCGTTAATTGAAGGAAAAACACTCCATCCGGAGTTGATCTCTAGC
CAGTTCAGTAGCGATGAAATGTGTATTATCGTTAGTAATCAACACCCTCTCAATTCAAAAGAAAAAGTGATTTTAAGCGA
TTTAGAAGATAGTCATTGGATTTTACGTGAGTCTGGTTCAGGCACTCGCGAGTTTTTCCTGCGTGCCGTCGCACCACGCA
TCGAACATTGGTATGAATCCTTTGAGCTCAACACGACTGAGGCGATCATCAATTCTGTTTCTGCCAATCTTGGATTCGCG
TGTTTATCACGGCTGGCTGCGCAACGAGCAATTGACTCAGGCCGAGTGAAAGCACTCGATGTCCCACTCGACATGAAACG
TCGATTCTGGATGCTGGTCCATAAAGACAAATACCAGAGCCCACTGCTGAAATCTTTCATGAGTTATTGTGAAGACTGGG
CTACACATCAAGATTGA

Upstream 100 bases:

>100_bases
ATACGCTTGCACTTCGTTAAACAATATCCAATTATATATAACAAGCGTTCATTAATACTGAACGACACCTTTCCACCAGC
AGCGCAAAACCAGGAACCTG

Downstream 100 bases:

>100_bases
GGCCGCATTGTTAGGGCTCCAACTGGACTTTTAACATCAGAAACTGTATAAAAAGCCAGTGAAATTTTATCAAACTTAGA
GGATTAACCATGGCTGTTAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MANISIKQLKVFVTITQHSTLTAASEALFLSKAAVSMALGEMEKQLGHSLFDRVNNRLILNQEGQKLLPLADEILHRAAG
IDVLFRDDQPLSGNLKVGASDTIGNQVAPFILSGFRELTQHQDQSLFISNSALICQKLVDYELDIALIEGKTLHPELISS
QFSSDEMCIIVSNQHPLNSKEKVILSDLEDSHWILRESGSGTREFFLRAVAPRIEHWYESFELNTTEAIINSVSANLGFA
CLSRLAAQRAIDSGRVKALDVPLDMKRRFWMLVHKDKYQSPLLKSFMSYCEDWATHQD

Sequences:

>Translated_298_residues
MANISIKQLKVFVTITQHSTLTAASEALFLSKAAVSMALGEMEKQLGHSLFDRVNNRLILNQEGQKLLPLADEILHRAAG
IDVLFRDDQPLSGNLKVGASDTIGNQVAPFILSGFRELTQHQDQSLFISNSALICQKLVDYELDIALIEGKTLHPELISS
QFSSDEMCIIVSNQHPLNSKEKVILSDLEDSHWILRESGSGTREFFLRAVAPRIEHWYESFELNTTEAIINSVSANLGFA
CLSRLAAQRAIDSGRVKALDVPLDMKRRFWMLVHKDKYQSPLLKSFMSYCEDWATHQD
>Mature_297_residues
ANISIKQLKVFVTITQHSTLTAASEALFLSKAAVSMALGEMEKQLGHSLFDRVNNRLILNQEGQKLLPLADEILHRAAGI
DVLFRDDQPLSGNLKVGASDTIGNQVAPFILSGFRELTQHQDQSLFISNSALICQKLVDYELDIALIEGKTLHPELISSQ
FSSDEMCIIVSNQHPLNSKEKVILSDLEDSHWILRESGSGTREFFLRAVAPRIEHWYESFELNTTEAIINSVSANLGFAC
LSRLAAQRAIDSGRVKALDVPLDMKRRFWMLVHKDKYQSPLLKSFMSYCEDWATHQD

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=293, Percent_Identity=36.518771331058, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI157672245, Length=304, Percent_Identity=25, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788748, Length=248, Percent_Identity=24.5967741935484, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87082132, Length=300, Percent_Identity=20, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1790399, Length=300, Percent_Identity=21.3333333333333, Blast_Score=61, Evalue=7e-11,
Organism=Escherichia coli, GI145693105, Length=298, Percent_Identity=21.4765100671141, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33458; Mature: 33326

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANISIKQLKVFVTITQHSTLTAASEALFLSKAAVSMALGEMEKQLGHSLFDRVNNRLIL
CCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
NQEGQKLLPLADEILHRAAGIDVLFRDDQPLSGNLKVGASDTIGNQVAPFILSGFRELTQ
CCCCCEEHHHHHHHHHHHCCCEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHH
HQDQSLFISNSALICQKLVDYELDIALIEGKTLHPELISSQFSSDEMCIIVSNQHPLNSK
CCCCEEEEECHHHHHHHHHCCCEEEEEECCCCCCHHHHHHCCCCCCEEEEECCCCCCCCC
EKVILSDLEDSHWILRESGSGTREFFLRAVAPRIEHWYESFELNTTEAIINSVSANLGFA
CEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHH
CLSRLAAQRAIDSGRVKALDVPLDMKRRFWMLVHKDKYQSPLLKSFMSYCEDWATHQD
HHHHHHHHHHHCCCCEEEEECCHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ANISIKQLKVFVTITQHSTLTAASEALFLSKAAVSMALGEMEKQLGHSLFDRVNNRLIL
CCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
NQEGQKLLPLADEILHRAAGIDVLFRDDQPLSGNLKVGASDTIGNQVAPFILSGFRELTQ
CCCCCEEHHHHHHHHHHHCCCEEEEECCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHH
HQDQSLFISNSALICQKLVDYELDIALIEGKTLHPELISSQFSSDEMCIIVSNQHPLNSK
CCCCEEEEECHHHHHHHHHCCCEEEEEECCCCCCHHHHHHCCCCCCEEEEECCCCCCCCC
EKVILSDLEDSHWILRESGSGTREFFLRAVAPRIEHWYESFELNTTEAIINSVSANLGFA
CEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHH
CLSRLAAQRAIDSGRVKALDVPLDMKRRFWMLVHKDKYQSPLLKSFMSYCEDWATHQD
HHHHHHHHHHHCCCCEEEEECCHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]