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The map label for this gene is grcA [H]

Identifier: 218708533

GI number: 218708533

Start: 518172

End: 518549

Strand: Reverse

Name: grcA [H]

Synonym: VS_0498

Alternate gene names: 218708533

Gene position: 518549-518172 (Counterclockwise)

Preceding gene: 218708537

Following gene: 218708530

Centisome position: 15.72

GC content: 45.5

Gene sequence:

>378_bases
ATGATCCAAGGTATTCAAATTACTAAAGCAGCAAACGATGACCTACTAAACTCAATCTGGCTACTAGATAGCGAGAAGAA
TGAAGCTCGTTGTGTTGTTGCTACTTCAGGTTTCGAAGCTGACCAAGTTATCTCAGCTGCAGACCTAGGCGAGTACGAAA
GCCGTGAAGTTGCTATCGAAGCAGCACCACGCATCGAAGGTGGTCAACACCTAAACGTTAACGTTCTTAAGCGTGAAACG
CTAGAAGATGCTGTTGCTCACCCAGAGAACTACCCACAGCTAACTATCCGTGTATCGGGTTACGCTGTACGTTTCAACTC
TCTAACTACTGAACAGCAACGTGACGTTATCGCACGTACGTTCACTGAGTCTCTATAA

Upstream 100 bases:

>100_bases
AATATTAAACTACCACAAACTAGGTATACATTACTCAGCTCAATAAAATTCGAAAGTTAACACCATAATCAAACCACATA
AAGTGGACTAGGAGATAGTT

Downstream 100 bases:

>100_bases
TTTTTAGAGCTTGAATTAGTGACCCGTCCTGATATGGGGTGACACTTGATTGACTAAAACGCTCGATGTACTTCATCGGG
CGTTTTGTATTTTAGAGCTG

Product: autonomous glycyl radical cofactor GrcA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 125; Mature: 125

Protein sequence:

>125_residues
MIQGIQITKAANDDLLNSIWLLDSEKNEARCVVATSGFEADQVISAADLGEYESREVAIEAAPRIEGGQHLNVNVLKRET
LEDAVAHPENYPQLTIRVSGYAVRFNSLTTEQQRDVIARTFTESL

Sequences:

>Translated_125_residues
MIQGIQITKAANDDLLNSIWLLDSEKNEARCVVATSGFEADQVISAADLGEYESREVAIEAAPRIEGGQHLNVNVLKRET
LEDAVAHPENYPQLTIRVSGYAVRFNSLTTEQQRDVIARTFTESL
>Mature_125_residues
MIQGIQITKAANDDLLNSIWLLDSEKNEARCVVATSGFEADQVISAADLGEYESREVAIEAAPRIEGGQHLNVNVLKRET
LEDAVAHPENYPQLTIRVSGYAVRFNSLTTEQQRDVIARTFTESL

Specific function: Acts as a radical domain for damaged PFL and possibly other radical proteins [H]

COG id: COG3445

COG function: function code R; Acid-induced glycyl radical enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glycine radical domain [H]

Homologues:

Organism=Escherichia coli, GI1788933, Length=127, Percent_Identity=77.1653543307087, Blast_Score=206, Evalue=4e-55,
Organism=Escherichia coli, GI1787131, Length=61, Percent_Identity=78.6885245901639, Blast_Score=105, Evalue=1e-24,
Organism=Escherichia coli, GI48994926, Length=61, Percent_Identity=75.4098360655738, Blast_Score=103, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR011140 [H]

Pfam domain/function: PF01228 Gly_radical [H]

EC number: NA

Molecular weight: Translated: 13847; Mature: 13847

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQGIQITKAANDDLLNSIWLLDSEKNEARCVVATSGFEADQVISAADLGEYESREVAIE
CCCCEEEEECCCCCHHHEEEEEECCCCCCEEEEEECCCCHHHEEHHHCCCCCCCCEEEEE
AAPRIEGGQHLNVNVLKRETLEDAVAHPENYPQLTIRVSGYAVRFNSLTTEQQRDVIART
ECCCCCCCCEEEEEEEHHHHHHHHHCCCCCCCEEEEEECCEEEEECCCCCHHHHHHHHHH
FTESL
HHHCC
>Mature Secondary Structure
MIQGIQITKAANDDLLNSIWLLDSEKNEARCVVATSGFEADQVISAADLGEYESREVAIE
CCCCEEEEECCCCCHHHEEEEEECCCCCCEEEEEECCCCHHHEEHHHCCCCCCCCEEEEE
AAPRIEGGQHLNVNVLKRETLEDAVAHPENYPQLTIRVSGYAVRFNSLTTEQQRDVIART
ECCCCCCCCEEEEEEEHHHHHHHHHCCCCCCCEEEEEECCEEEEECCCCCHHHHHHHHHH
FTESL
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA