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The map label for this gene is gltD [H]

Identifier: 218708506

GI number: 218708506

Start: 485040

End: 486485

Strand: Reverse

Name: gltD [H]

Synonym: VS_0470

Alternate gene names: 218708506

Gene position: 486485-485040 (Counterclockwise)

Preceding gene: 218708507

Following gene: 218708505

Centisome position: 14.75

GC content: 48.27

Gene sequence:

>1446_bases
GTGTTCAAGCGCAATAATTGGAAGGAGCCAATAATGAGCCAGAATGTATACCAATTTATTGATGTGAATCGCGTAGACCC
AGCGAAGAAACCAATCAAGATACGTAAGATTGAGTTTGTAGAGATCTACGAACCTTTCACTAAGCAACAAGCCAAGGCGC
AAGCTGACCGCTGTCTAGACTGTGGTAATCCTTACTGTGAATGGAAATGTCCAGTCCATAACTACATCCCTCAGTGGCTT
AAGCTTGCCAATGAAGGACGCATTATCGAAGCTGCTGAACTGTCCCACCAAACCAACAGCTTACCTGAGGTGTGTGGACG
AGTTTGCCCTCAAGATCGACTGTGCGAAGGATCTTGTACCCTCAATGATGATTTCGGCGCAGTAACCATCGGCAACATTG
AAAAATACATCAATGACAAAGCGTTTGAGATGGGCTGGAAGCCAGACATGTCTCACGTTGAATGGACCGACAAAAAGGTA
GCTATCATCGGCGCAGGCCCAGCAGGTCTTGCTGCTGCTGATATCTTAGTTCGAAACGGTGTGAAGGCCGTGGTATTTGA
CCGCTACCCCGAGATCGGTGGCTTGCTAACGTTTGGTATCCCATCGTTCAAACTTGAGAAAGGCATCATGGAAAACCGTC
GCCGTATCTTTAGTGGTATGGGTGTCGAATTTAAAATGAATACCGAAGTCGGCAAAGACGTTCAACTGCAACAACTGGTC
GATGACTATGATGCTGTTTTCTTGGGCGTCGGCACCTATAAAAATATGCGTGCTGGGTTAGACAACGAAGAAGCACCGGG
CGTTTATGATGCCCTACCATTTCTTGTTTCCAACACCTACAAGGTCATGGAGCTGGATAACCCAACGCCATTCATCGACA
TGAAAGGCAAAAAAGTGGTTGTGCTTGGTGGTGGTGATACCGCGATGGACTGTGTCCGAACGTCGATTCGTCAACACGCT
GCTAATGTTGTTTGTGCTTATCGCCGAGATGAAGCGAACATGCCCGGCTCTCGCCGAGAGGTGAAAAATGCAAAAGAAGA
AGGCGTGAAGTTCATGTTCAACCTTCAGCCATTAGCATTGGAAACCGATTCATCCGGTCATGTGACTGGCGTAAAAGTGG
TGAAAACCGCTTTGGGTGAACCTGATGAAGCAGGCCGTCGTCGCCCTGAACCCGTAGAAGGCAGTGAGCATGTTCTTGAG
GCTGATGCCGTGATCATGGCATTTGGTTTCCAACCTCATGCAATGGAATGGCTAAAACCATTTGGTGTAGAGCTCGACCA
ATGGGGGCGCATCAAAGCTCCCGGCAAACAAGAGTTTCAATACCAAACCACCAACAGCAAGATCTTTGCAGGTGGTGATG
CGGTTCGAGGATCTGACTTGGTGGTGACCGCTATCGATGAAGGCCGTAAAGCCGCAGAAGGGATATTGGACTACCTAGAA
GTGTGA

Upstream 100 bases:

>100_bases
AACTTTGATGAATGGATTCCGAAGTTCTACCTAGTGAAGCCAAAGACGGCGGATCTTAACACCTTGCTCGGTCACCAAAG
CCGTAGCTCTGCTGAACTTC

Downstream 100 bases:

>100_bases
TTGGGTTTCCTTTATTACTCAGTTCGTTACTATAAAAAAAGAATCCAATGACGGATCCTTTTTTGAATTAGATACGTTTA
TCACAAGCATAACGAAAATA

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MFKRNNWKEPIMSQNVYQFIDVNRVDPAKKPIKIRKIEFVEIYEPFTKQQAKAQADRCLDCGNPYCEWKCPVHNYIPQWL
KLANEGRIIEAAELSHQTNSLPEVCGRVCPQDRLCEGSCTLNDDFGAVTIGNIEKYINDKAFEMGWKPDMSHVEWTDKKV
AIIGAGPAGLAAADILVRNGVKAVVFDRYPEIGGLLTFGIPSFKLEKGIMENRRRIFSGMGVEFKMNTEVGKDVQLQQLV
DDYDAVFLGVGTYKNMRAGLDNEEAPGVYDALPFLVSNTYKVMELDNPTPFIDMKGKKVVVLGGGDTAMDCVRTSIRQHA
ANVVCAYRRDEANMPGSRREVKNAKEEGVKFMFNLQPLALETDSSGHVTGVKVVKTALGEPDEAGRRRPEPVEGSEHVLE
ADAVIMAFGFQPHAMEWLKPFGVELDQWGRIKAPGKQEFQYQTTNSKIFAGGDAVRGSDLVVTAIDEGRKAAEGILDYLE
V

Sequences:

>Translated_481_residues
MFKRNNWKEPIMSQNVYQFIDVNRVDPAKKPIKIRKIEFVEIYEPFTKQQAKAQADRCLDCGNPYCEWKCPVHNYIPQWL
KLANEGRIIEAAELSHQTNSLPEVCGRVCPQDRLCEGSCTLNDDFGAVTIGNIEKYINDKAFEMGWKPDMSHVEWTDKKV
AIIGAGPAGLAAADILVRNGVKAVVFDRYPEIGGLLTFGIPSFKLEKGIMENRRRIFSGMGVEFKMNTEVGKDVQLQQLV
DDYDAVFLGVGTYKNMRAGLDNEEAPGVYDALPFLVSNTYKVMELDNPTPFIDMKGKKVVVLGGGDTAMDCVRTSIRQHA
ANVVCAYRRDEANMPGSRREVKNAKEEGVKFMFNLQPLALETDSSGHVTGVKVVKTALGEPDEAGRRRPEPVEGSEHVLE
ADAVIMAFGFQPHAMEWLKPFGVELDQWGRIKAPGKQEFQYQTTNSKIFAGGDAVRGSDLVVTAIDEGRKAAEGILDYLE
V
>Mature_481_residues
MFKRNNWKEPIMSQNVYQFIDVNRVDPAKKPIKIRKIEFVEIYEPFTKQQAKAQADRCLDCGNPYCEWKCPVHNYIPQWL
KLANEGRIIEAAELSHQTNSLPEVCGRVCPQDRLCEGSCTLNDDFGAVTIGNIEKYINDKAFEMGWKPDMSHVEWTDKKV
AIIGAGPAGLAAADILVRNGVKAVVFDRYPEIGGLLTFGIPSFKLEKGIMENRRRIFSGMGVEFKMNTEVGKDVQLQQLV
DDYDAVFLGVGTYKNMRAGLDNEEAPGVYDALPFLVSNTYKVMELDNPTPFIDMKGKKVVVLGGGDTAMDCVRTSIRQHA
ANVVCAYRRDEANMPGSRREVKNAKEEGVKFMFNLQPLALETDSSGHVTGVKVVKTALGEPDEAGRRRPEPVEGSEHVLE
ADAVIMAFGFQPHAMEWLKPFGVELDQWGRIKAPGKQEFQYQTTNSKIFAGGDAVRGSDLVVTAIDEGRKAAEGILDYLE
V

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=480, Percent_Identity=28.5416666666667, Blast_Score=156, Evalue=4e-38,
Organism=Escherichia coli, GI1789606, Length=472, Percent_Identity=75.8474576271186, Blast_Score=755, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=475, Percent_Identity=63.7894736842105, Blast_Score=644, Evalue=0.0,
Organism=Escherichia coli, GI87082180, Length=450, Percent_Identity=57.7777777777778, Blast_Score=550, Evalue=1e-157,
Organism=Escherichia coli, GI1788468, Length=443, Percent_Identity=30.6997742663657, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1789243, Length=432, Percent_Identity=33.3333333333333, Blast_Score=163, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17570289, Length=487, Percent_Identity=33.264887063655, Blast_Score=226, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI71984108, Length=470, Percent_Identity=28.936170212766, Blast_Score=152, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17543860, Length=159, Percent_Identity=30.188679245283, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6320030, Length=465, Percent_Identity=32.9032258064516, Blast_Score=226, Evalue=4e-60,
Organism=Drosophila melanogaster, GI28574881, Length=484, Percent_Identity=38.0165289256198, Blast_Score=281, Evalue=6e-76,
Organism=Drosophila melanogaster, GI24665539, Length=484, Percent_Identity=38.0165289256198, Blast_Score=281, Evalue=6e-76,
Organism=Drosophila melanogaster, GI24665547, Length=484, Percent_Identity=38.0165289256198, Blast_Score=281, Evalue=7e-76,
Organism=Drosophila melanogaster, GI24665543, Length=484, Percent_Identity=38.0165289256198, Blast_Score=281, Evalue=7e-76,
Organism=Drosophila melanogaster, GI24640763, Length=468, Percent_Identity=27.1367521367521, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI18858217, Length=468, Percent_Identity=27.1367521367521, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI17137178, Length=159, Percent_Identity=30.188679245283, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 53611; Mature: 53611

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKRNNWKEPIMSQNVYQFIDVNRVDPAKKPIKIRKIEFVEIYEPFTKQQAKAQADRCLD
CCCCCCCCCCHHCCCHHHHHCCCCCCCCCCCEEEEEEEEEEHHCHHHHHHHHHHHHHHHH
CGNPYCEWKCPVHNYIPQWLKLANEGRIIEAAELSHQTNSLPEVCGRVCPQDRLCEGSCT
CCCCCEEEECCHHHHHHHHHHHHCCCCEEEHHHHCCCCCCHHHHHHHHCCCCCCCCCCCE
LNDDFGAVTIGNIEKYINDKAFEMGWKPDMSHVEWTDKKVAIIGAGPAGLAAADILVRNG
ECCCCCEEEECCHHHHHCCHHHCCCCCCCCCCCEECCCEEEEEECCCHHHHHHHHHHHCC
VKAVVFDRYPEIGGLLTFGIPSFKLEKGIMENRRRIFSGMGVEFKMNTEVGKDVQLQQLV
CEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHH
DDYDAVFLGVGTYKNMRAGLDNEEAPGVYDALPFLVSNTYKVMELDNPTPFIDMKGKKVV
CCCCEEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCEEEECCCEEE
VLGGGDTAMDCVRTSIRQHAANVVCAYRRDEANMPGSRREVKNAKEEGVKFMFNLQPLAL
EECCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCEEEE
ETDSSGHVTGVKVVKTALGEPDEAGRRRPEPVEGSEHVLEADAVIMAFGFQPHAMEWLKP
ECCCCCCEEHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHHHCEEEEEECCCHHHHHHHHH
FGVELDQWGRIKAPGKQEFQYQTTNSKIFAGGDAVRGSDLVVTAIDEGRKAAEGILDYLE
CCCCCCCCCCCCCCCCCCCEEEECCCEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHC
V
C
>Mature Secondary Structure
MFKRNNWKEPIMSQNVYQFIDVNRVDPAKKPIKIRKIEFVEIYEPFTKQQAKAQADRCLD
CCCCCCCCCCHHCCCHHHHHCCCCCCCCCCCEEEEEEEEEEHHCHHHHHHHHHHHHHHHH
CGNPYCEWKCPVHNYIPQWLKLANEGRIIEAAELSHQTNSLPEVCGRVCPQDRLCEGSCT
CCCCCEEEECCHHHHHHHHHHHHCCCCEEEHHHHCCCCCCHHHHHHHHCCCCCCCCCCCE
LNDDFGAVTIGNIEKYINDKAFEMGWKPDMSHVEWTDKKVAIIGAGPAGLAAADILVRNG
ECCCCCEEEECCHHHHHCCHHHCCCCCCCCCCCEECCCEEEEEECCCHHHHHHHHHHHCC
VKAVVFDRYPEIGGLLTFGIPSFKLEKGIMENRRRIFSGMGVEFKMNTEVGKDVQLQQLV
CEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHH
DDYDAVFLGVGTYKNMRAGLDNEEAPGVYDALPFLVSNTYKVMELDNPTPFIDMKGKKVV
CCCCEEEEECCCCHHHHCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCEEEECCCEEE
VLGGGDTAMDCVRTSIRQHAANVVCAYRRDEANMPGSRREVKNAKEEGVKFMFNLQPLAL
EECCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCEEEE
ETDSSGHVTGVKVVKTALGEPDEAGRRRPEPVEGSEHVLEADAVIMAFGFQPHAMEWLKP
ECCCCCCEEHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHHHCEEEEEECCCHHHHHHHHH
FGVELDQWGRIKAPGKQEFQYQTTNSKIFAGGDAVRGSDLVVTAIDEGRKAAEGILDYLE
CCCCCCCCCCCCCCCCCCCEEEECCCEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHC
V
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]