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The map label for this gene is mtnN

Identifier: 218708504

GI number: 218708504

Start: 483617

End: 484312

Strand: Reverse

Name: mtnN

Synonym: VS_0468

Alternate gene names: 218708504

Gene position: 484312-483617 (Counterclockwise)

Preceding gene: 218708505

Following gene: 218708503

Centisome position: 14.68

GC content: 45.98

Gene sequence:

>696_bases
ATGAAAATCGGCATCATTGGCGCAATGGAGCAAGAAGTTGCGATCCTAAAAGCAGCAATTTCAGACATTACTGAAGTTAA
TAAAGGCGGTTGTACCTTTTTCTCTGGTCAGCTAAATGGTGTTGACGTTGTTTTGCTTCAATCAGGCATTGGTAAAGTAG
CAGCGGCTGTTGGTACTTCAATCCTGCTAAGCGAATACCAACCAGATGTAGTATTAAATACTGGCTCTGCTGGCGGTTTT
GATTCAACACTGAACCTTGGCGATGTGGTTATCTCTACTGAAGTTCGTCACCACGATGCTGACGTTACAGCTTTCGGTTA
CGAAATTGGCCAAATGGCAGGTCAACCAGCAGCGTTCAAAGCTGACGACAAACTGATGGCTGTTGCTGAACAAGCGCTAG
CACAAATGGAAGATAAGCATGCCGTTCGCGGTCTTATCTGTACTGGCGACGCATTTGTTTGCACAGCAGAACGTCAAGAG
TTCATCCGCAAGCACTTCCCATCTGTCGTTGCTGTAGAGATGGAAGCATCGGCTATCGCTCAAGCTTGTCACCAATTCCA
AGTACCATTCGTGGTTGTTCGTGCAATCTCTGACGTTGCAGACAAAGAATCACCAATGAGCTTCGAAGAGTTCCTGCCAC
TAGCAGCGCAAAGCTCTTCTGAAATGGTTATCAAGATGGTTGCCCTACTAAAGTAA

Upstream 100 bases:

>100_bases
GTATTCGGATGAGAAGTGCTTCTGGGCAGTCGCTCGACACTTCTCTTCCATGCTACAATTTCGCTCTACTGTTTAAATAA
TTTTTATTAAGAGACTACAC

Downstream 100 bases:

>100_bases
GCGACTAGACATGCAAACACTGTTTGATCAGGTATTTGCAAATGGCGTGCTCCTTGTGATGTGGGGAGCACTGCTTTTTC
ATTTAATTTTACCTATCCCC

Product: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase

Products: NA

Alternate protein names: MTA/SAH nucleosidase; MTAN; 5'-methylthioadenosine nucleosidase; MTA nucleosidase; S-adenosylhomocysteine nucleosidase; AdoHcy nucleosidase; SAH nucleosidase; SRH nucleosidase

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MKIGIIGAMEQEVAILKAAISDITEVNKGGCTFFSGQLNGVDVVLLQSGIGKVAAAVGTSILLSEYQPDVVLNTGSAGGF
DSTLNLGDVVISTEVRHHDADVTAFGYEIGQMAGQPAAFKADDKLMAVAEQALAQMEDKHAVRGLICTGDAFVCTAERQE
FIRKHFPSVVAVEMEASAIAQACHQFQVPFVVVRAISDVADKESPMSFEEFLPLAAQSSSEMVIKMVALLK

Sequences:

>Translated_231_residues
MKIGIIGAMEQEVAILKAAISDITEVNKGGCTFFSGQLNGVDVVLLQSGIGKVAAAVGTSILLSEYQPDVVLNTGSAGGF
DSTLNLGDVVISTEVRHHDADVTAFGYEIGQMAGQPAAFKADDKLMAVAEQALAQMEDKHAVRGLICTGDAFVCTAERQE
FIRKHFPSVVAVEMEASAIAQACHQFQVPFVVVRAISDVADKESPMSFEEFLPLAAQSSSEMVIKMVALLK
>Mature_231_residues
MKIGIIGAMEQEVAILKAAISDITEVNKGGCTFFSGQLNGVDVVLLQSGIGKVAAAVGTSILLSEYQPDVVLNTGSAGGF
DSTLNLGDVVISTEVRHHDADVTAFGYEIGQMAGQPAAFKADDKLMAVAEQALAQMEDKHAVRGLICTGDAFVCTAERQE
FIRKHFPSVVAVEMEASAIAQACHQFQVPFVVVRAISDVADKESPMSFEEFLPLAAQSSSEMVIKMVALLK

Specific function: Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S- adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively

COG id: COG0775

COG function: function code F; Nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/UDP phosphorylase family. MtnN subfamily

Homologues:

Organism=Escherichia coli, GI1786354, Length=230, Percent_Identity=57.8260869565217, Blast_Score=246, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTNN_VIBSL (B7VJ21)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002416125.1
- GeneID:   7160103
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_0468
- HOGENOM:   HBG367723
- OMA:   AMEQEVT
- ProtClustDB:   PRK05584
- HAMAP:   MF_01684
- InterPro:   IPR010049
- InterPro:   IPR018017
- InterPro:   IPR000845
- PANTHER:   PTHR21234
- TIGRFAMs:   TIGR01704

Pfam domain/function: PF01048 PNP_UDP_1

EC number: =3.2.2.9

Molecular weight: Translated: 24466; Mature: 24466

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: ACT_SITE 12-12 BINDING 78-78 BINDING 198-198

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGIIGAMEQEVAILKAAISDITEVNKGGCTFFSGQLNGVDVVLLQSGIGKVAAAVGTS
CEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEEHHCCHHHHHHHHHH
ILLSEYQPDVVLNTGSAGGFDSTLNLGDVVISTEVRHHDADVTAFGYEIGQMAGQPAAFK
HHHHCCCCCEEEECCCCCCCCCCCCCCCEEEEEEHHHCCCCEEEHHHHHHHHCCCCCCCC
ADDKLMAVAEQALAQMEDKHAVRGLICTGDAFVCTAERQEFIRKHFPSVVAVEMEASAIA
CCHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEEEHHHHHHHHHHCCCEEEEEHHHHHHH
QACHQFQVPFVVVRAISDVADKESPMSFEEFLPLAAQSSSEMVIKMVALLK
HHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHCCHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKIGIIGAMEQEVAILKAAISDITEVNKGGCTFFSGQLNGVDVVLLQSGIGKVAAAVGTS
CEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEEHHCCHHHHHHHHHH
ILLSEYQPDVVLNTGSAGGFDSTLNLGDVVISTEVRHHDADVTAFGYEIGQMAGQPAAFK
HHHHCCCCCEEEECCCCCCCCCCCCCCCEEEEEEHHHCCCCEEEHHHHHHHHCCCCCCCC
ADDKLMAVAEQALAQMEDKHAVRGLICTGDAFVCTAERQEFIRKHFPSVVAVEMEASAIA
CCHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEEEHHHHHHHHHHCCCEEEEEHHHHHHH
QACHQFQVPFVVVRAISDVADKESPMSFEEFLPLAAQSSSEMVIKMVALLK
HHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHCCHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA