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The map label for this gene is yahB [H]

Identifier: 218703601

GI number: 218703601

Start: 386638

End: 387570

Strand: Reverse

Name: yahB [H]

Synonym: ECUMN_0355

Alternate gene names: 218703601

Gene position: 387570-386638 (Counterclockwise)

Preceding gene: 218703602

Following gene: 218703599

Centisome position: 7.45

GC content: 49.84

Gene sequence:

>933_bases
ATGAACTCAATTTTTACCGAAGAGAATCTGTTGGCATTTACTACCGCTGCGCGTTTTGGCAGCTTCAGTAAAGCCGCAGA
AGAGTTGGGTTTAACCACTTCCGCCATTAGCTACACCATCAAGCGAATGGAAACAGGGCTGGATGTGGTGCTGTTCACTC
GCAGTACCCGCAGCATTGAGTTAACGGAGTCCGGGCGTTATTTTTTCCGCAAAGCTACCGACCTGCTGAATGATTTTCAC
GCCATCAAACGCAGCATCGATACTATTTCGCAGGGTATAGAAGCACGGGTGCGCATTTGTATTAACCAACTGCTTTATAC
GCCCAAACACACCGCACGCTTATTACAGGTACTGAAAAAGCAGTTTCCAACCTGCCAGATTACCGTGACGACCGAAGTGT
ATAACGGCGTCTGGGATGCGATTATTAATAATCAGGCGAATATTGCTATCGGCGCACCCGACACGCTGCTGGACGGTGGC
GGCATTGATTACACGGAGATTGGCGCGATTCGCTGGGCTTTTGCTATCGCCCCGGATCACCCGTTAGCCTTTGTGCCGGA
ACCCATCGCCGAAAGCCAGCTGCGTCTCTATCCCAATATCATGGTGGAAGACACCGCGCATACGATTAATAAAAAAGTGG
GCTGGTTGCTGCATGGGCAGGAGTCGATTCTGGTGCCAGATTTCAACACCAAATGTCAGTGCCAGATTTTAGGTGAAGGA
ATTGGTTTTTTGCCGGATTACATGGTTCGCGAGGCGATGGAGCAATCCCTGCTGGTTACCCGCCAAATCCATAATCCGCG
CCAGGATTCACGCATGTTACTGGCGACCCAGCACTCTGCCACCGGTCAGGTGACGCAGTGGATTAAAAAACAATTTTCGC
CAAATGGCATATTAACGGGGATCTATCAGGATTTACTGCATCGGGAGAATTAA

Upstream 100 bases:

>100_bases
GGGTATTAAAAGTTATTCAAAAATGATTATCTATCTTTAACCGATACCTCTACAGTCATGGCTGGAGAAATTCATATTCG
ATAATGGCGGGAATGGTTTA

Downstream 100 bases:

>100_bases
TCGCTGCCAACCTCCCGATGCGCTGTGAATAGCGGTTTACATCAACCACCTGCTTTCATTACCCATTCAGAGATAAATTT
ATTACCCGGTACTGGCGGAG

Product: putative DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRMETGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFH
AIKRSIDTISQGIEARVRICINQLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLHGQESILVPDFNTKCQCQILGEG
IGFLPDYMVREAMEQSLLVTRQIHNPRQDSRMLLATQHSATGQVTQWIKKQFSPNGILTGIYQDLLHREN

Sequences:

>Translated_310_residues
MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRMETGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFH
AIKRSIDTISQGIEARVRICINQLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLHGQESILVPDFNTKCQCQILGEG
IGFLPDYMVREAMEQSLLVTRQIHNPRQDSRMLLATQHSATGQVTQWIKKQFSPNGILTGIYQDLLHREN
>Mature_310_residues
MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRMETGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFH
AIKRSIDTISQGIEARVRICINQLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLHGQESILVPDFNTKCQCQILGEG
IGFLPDYMVREAMEQSLLVTRQIHNPRQDSRMLLATQHSATGQVTQWIKKQFSPNGILTGIYQDLLHREN

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786508, Length=310, Percent_Identity=98.7096774193548, Blast_Score=638, Evalue=0.0,
Organism=Escherichia coli, GI1786713, Length=290, Percent_Identity=38.2758620689655, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1787949, Length=285, Percent_Identity=30.1754385964912, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI87082024, Length=293, Percent_Identity=25.938566552901, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1789492, Length=291, Percent_Identity=28.5223367697594, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786401, Length=263, Percent_Identity=26.2357414448669, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI145693105, Length=175, Percent_Identity=25.1428571428571, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI157672245, Length=188, Percent_Identity=27.6595744680851, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787589, Length=275, Percent_Identity=26.5454545454545, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789639, Length=147, Percent_Identity=27.891156462585, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34815; Mature: 34815

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRMETGLDVVLFTRSTRSIE
CCCCCCCCCEEEEEHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCCEEE
LTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICINQLLYTPKHTARLLQVLKK
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
QFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGGGIDYTEIGAIRWAFAIAPDH
HCCEEEEEEEHHHHHHHHHHHHCCCCCEEECCCCCEECCCCCCHHHHHHEEEEEEECCCC
PLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLHGQESILVPDFNTKCQCQILGEG
CCEECCCCHHCCHHEECCCEEEECHHHHHHHHHCEEEECCCEEEECCCCCCEEEEEECCC
IGFLPDYMVREAMEQSLLVTRQIHNPRQDSRMLLATQHSATGQVTQWIKKQFSPNGILTG
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCHHH
IYQDLLHREN
HHHHHHHCCC
>Mature Secondary Structure
MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRMETGLDVVLFTRSTRSIE
CCCCCCCCCEEEEEHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCCCEEE
LTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICINQLLYTPKHTARLLQVLKK
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
QFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGGGIDYTEIGAIRWAFAIAPDH
HCCEEEEEEEHHHHHHHHHHHHCCCCCEEECCCCCEECCCCCCHHHHHHEEEEEEECCCC
PLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLHGQESILVPDFNTKCQCQILGEG
CCEECCCCHHCCHHEECCCEEEECHHHHHHHHHCEEEECCCEEEECCCCCCEEEEEECCC
IGFLPDYMVREAMEQSLLVTRQIHNPRQDSRMLLATQHSATGQVTQWIKKQFSPNGILTG
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCHHH
IYQDLLHREN
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]