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The map label for this gene is yfkN [H]

Identifier: 218701353

GI number: 218701353

Start: 3177991

End: 3179616

Strand: Direct

Name: yfkN [H]

Synonym: ECIAI39_3052

Alternate gene names: 218701353

Gene position: 3177991-3179616 (Clockwise)

Preceding gene: 218701352

Following gene: 218701354

Centisome position: 61.92

GC content: 44.1

Gene sequence:

>1626_bases
ATGCGAATGAAAACAGTGCAGCGTACTTTACTTGCCGCAACATTGCTGACTATTTTTTCGGTCTCAGTCATGGCGCAGGA
TGTCACGATTATTTATACAAATGATCTTCACGCTCATGTGGATTCTTATAAAGTGCCGTATATCGCAGACGGAAAACGCG
ACATTGGCGGCTTTGCCAATATATCTACTTTAGTAAAACAAGAGAAAGCGAAAAATAAAGCCACCTTTTATTTTGATACT
GGAGACTATTTCACCGGCCCTTATATCAGCAGTCTGACAAAGGGCGAAGCAATAATTGACATTATGAATACCATGCCTTT
TGACGCAGTATCAATCGGTAATCATGAGTTTGACCATGGCTGGGATAATGCCCTGCGACAATTAAGCAAAGCGAATTTTC
CTGTTTTATTGGGCAATGTTTATCATAAAGAGAGTGAGAACCCCTTCTGGAACAAGCCCTACACCATCCTGGAAAAAGAT
GGCATCAAGATTGGTATTATTGGGCTACACGGCGTATTTGCATTTAATGATACGGTCTCGGAGCTCTCTCTCCAGGGACT
CGATAATGACAATAATAACCGCTTTGATAAATCTTCCGAGACCCTTAAGAATCAGGGGATTGAAGCACGTGATGAAGTGA
AGTATTTGCAGCATTATATCGACGAGTTACGTGATAAGGTTGACCTCACTGTGGCGCTGGTTCATGAAGGCGTTCCGGCG
CGTCAGTCCAGCATTGGCAATACAGATGTTAGACGCGCTCTTGATAAAGATATTCAAACCGCTAGCAAGGTGAAAGGATT
GGATATTCTGATTACCGGGCATGCCCACGTCGGAACGCCAGAACCAATCAAAGTCGGCAACACCTTAATCCTTTCGACTG
ATAGTGGCGGTATCGATATTGGTAAATTAGTCCTTGATGTCAACCCTACAGCTCGTACCCATAAAATGAAGAGCTTTGAG
CTGAAAACAGTTTATGCCGATGAATGGATACCCGATCCAACGACACAAAAAGTTATCAATGGCTGGAATAAAAAACTGGC
GGATATTGTACGCCAACCAGTAGGTGAATCATCGATTGCATTAACTCGCGCCTATGGTGAGTCATCGCAACTAGGCAACC
TGTTTACCGATGCAATGCTTGTCGCTGCTCCGACTGCACAGATTGCATTGATAAACTCCGGCAGCTTACGTGCGGATATA
AATGCAGGCACGATTACCTTTGGCGACATTACCAGTACATTCCCCTTCAAGAATGAACTTACTGAAATGGATCTCAGCGG
TAAGGATCTGCGTAACCTACTGGAACACGGGGCATCGCTTACCAATGGCATCCTGCAAATGTCAAAAGGCGCAGAAATGA
GCTATACCCCGCAGAAACCAGTAGGTCAGCGCATAGTATCTTTTAAAATTAATGGTGAAGAGATCGTTGATACGAATATT
TACCATGTTGCAACAACGACTTTCCTTGCACTGGAGGGCGATGGTTTTCTGGCATTCAAAGAAGGGAAAAATGTTCAGGT
CCGCGCCGGAAATAACATGTCCGATGTCGTGATCGATTATTTGAAGAAAGGCCACAAGATTACGCCTGCACAGGTAAATG
AAATGCGGGTGGACGTTAGCAAATAA

Upstream 100 bases:

>100_bases
TATTTCAAAAATCATAAACCTGTCACCCCAGACTTAACTAAACGAGTCATGGACGTCGCCAAATAAAATATCATTTATCA
GTCTATATATTTAACAAGGA

Downstream 100 bases:

>100_bases
TAAAAAAATTATAATGCCATTCATTCACTGAATGGCATTATTTTTCTCTAAATCAACAATAGATAATGAGATATATTTAT
GTGTATAAAAAAGCACGGGA

Product: putative 5'-nucleotidase/2',3'-cyclic phosphodiesterase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MRMKTVQRTLLAATLLTIFSVSVMAQDVTIIYTNDLHAHVDSYKVPYIADGKRDIGGFANISTLVKQEKAKNKATFYFDT
GDYFTGPYISSLTKGEAIIDIMNTMPFDAVSIGNHEFDHGWDNALRQLSKANFPVLLGNVYHKESENPFWNKPYTILEKD
GIKIGIIGLHGVFAFNDTVSELSLQGLDNDNNNRFDKSSETLKNQGIEARDEVKYLQHYIDELRDKVDLTVALVHEGVPA
RQSSIGNTDVRRALDKDIQTASKVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDIGKLVLDVNPTARTHKMKSFE
LKTVYADEWIPDPTTQKVINGWNKKLADIVRQPVGESSIALTRAYGESSQLGNLFTDAMLVAAPTAQIALINSGSLRADI
NAGTITFGDITSTFPFKNELTEMDLSGKDLRNLLEHGASLTNGILQMSKGAEMSYTPQKPVGQRIVSFKINGEEIVDTNI
YHVATTTFLALEGDGFLAFKEGKNVQVRAGNNMSDVVIDYLKKGHKITPAQVNEMRVDVSK

Sequences:

>Translated_541_residues
MRMKTVQRTLLAATLLTIFSVSVMAQDVTIIYTNDLHAHVDSYKVPYIADGKRDIGGFANISTLVKQEKAKNKATFYFDT
GDYFTGPYISSLTKGEAIIDIMNTMPFDAVSIGNHEFDHGWDNALRQLSKANFPVLLGNVYHKESENPFWNKPYTILEKD
GIKIGIIGLHGVFAFNDTVSELSLQGLDNDNNNRFDKSSETLKNQGIEARDEVKYLQHYIDELRDKVDLTVALVHEGVPA
RQSSIGNTDVRRALDKDIQTASKVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDIGKLVLDVNPTARTHKMKSFE
LKTVYADEWIPDPTTQKVINGWNKKLADIVRQPVGESSIALTRAYGESSQLGNLFTDAMLVAAPTAQIALINSGSLRADI
NAGTITFGDITSTFPFKNELTEMDLSGKDLRNLLEHGASLTNGILQMSKGAEMSYTPQKPVGQRIVSFKINGEEIVDTNI
YHVATTTFLALEGDGFLAFKEGKNVQVRAGNNMSDVVIDYLKKGHKITPAQVNEMRVDVSK
>Mature_541_residues
MRMKTVQRTLLAATLLTIFSVSVMAQDVTIIYTNDLHAHVDSYKVPYIADGKRDIGGFANISTLVKQEKAKNKATFYFDT
GDYFTGPYISSLTKGEAIIDIMNTMPFDAVSIGNHEFDHGWDNALRQLSKANFPVLLGNVYHKESENPFWNKPYTILEKD
GIKIGIIGLHGVFAFNDTVSELSLQGLDNDNNNRFDKSSETLKNQGIEARDEVKYLQHYIDELRDKVDLTVALVHEGVPA
RQSSIGNTDVRRALDKDIQTASKVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDIGKLVLDVNPTARTHKMKSFE
LKTVYADEWIPDPTTQKVINGWNKKLADIVRQPVGESSIALTRAYGESSQLGNLFTDAMLVAAPTAQIALINSGSLRADI
NAGTITFGDITSTFPFKNELTEMDLSGKDLRNLLEHGASLTNGILQMSKGAEMSYTPQKPVGQRIVSFKINGEEIVDTNI
YHVATTTFLALEGDGFLAFKEGKNVQVRAGNNMSDVVIDYLKKGHKITPAQVNEMRVDVSK

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=586, Percent_Identity=24.9146757679181, Blast_Score=115, Evalue=7e-26,
Organism=Escherichia coli, GI1790658, Length=631, Percent_Identity=22.662440570523, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1786687, Length=183, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI161076508, Length=489, Percent_Identity=25.7668711656442, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24652512, Length=489, Percent_Identity=25.7668711656442, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI19921980, Length=489, Percent_Identity=25.7668711656442, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24641187, Length=537, Percent_Identity=25.6983240223464, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19922446, Length=588, Percent_Identity=25.5102040816327, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24654424, Length=588, Percent_Identity=25.5102040816327, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI221329836, Length=561, Percent_Identity=24.5989304812834, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28573524, Length=584, Percent_Identity=25, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI19922444, Length=583, Percent_Identity=23.327615780446, Blast_Score=105, Evalue=8e-23,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 59541; Mature: 59541

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00786 5_NUCLEOTIDASE_2 ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMKTVQRTLLAATLLTIFSVSVMAQDVTIIYTNDLHAHVDSYKVPYIADGKRDIGGFAN
CCHHHHHHHHHHHHHHHHHHHHHEECCEEEEEECCCEEHHCCCCCCEEECCCCCCCCHHH
ISTLVKQEKAKNKATFYFDTGDYFTGPYISSLTKGEAIIDIMNTMPFDAVSIGNHEFDHG
HHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHCCCHHHHHHHHCCCCCEEECCCCCCCCC
WDNALRQLSKANFPVLLGNVYHKESENPFWNKPYTILEKDGIKIGIIGLHGVFAFNDTVS
HHHHHHHHHHCCCCEEECCEEECCCCCCCCCCCCEEEECCCCEEEEEEEEEEEEECCCHH
ELSLQGLDNDNNNRFDKSSETLKNQGIEARDEVKYLQHYIDELRDKVDLTVALVHEGVPA
HHEEECCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCC
RQSSIGNTDVRRALDKDIQTASKVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDI
HHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEECCCEEEEEECCCCCEE
GKLVLDVNPTARTHKMKSFELKTVYADEWIPDPTTQKVINGWNKKLADIVRQPVGESSIA
EEEEEECCCCHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEE
LTRAYGESSQLGNLFTDAMLVAAPTAQIALINSGSLRADINAGTITFGDITSTFPFKNEL
EEEECCCCCHHHHHHHHHHHEECCCEEEEEEECCCEEEECCCCEEEECCCCCCCCCCCCH
TEMDLSGKDLRNLLEHGASLTNGILQMSKGAEMSYTPQKPVGQRIVSFKINGEEIVDTNI
HHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCEEECCCE
YHVATTTFLALEGDGFLAFKEGKNVQVRAGNNMSDVVIDYLKKGHKITPAQVNEMRVDVS
EEEEEEEEEEEECCCEEEEECCCEEEEEECCCHHHHHHHHHHCCCEECCCCCCEEEEEEC
K
C
>Mature Secondary Structure
MRMKTVQRTLLAATLLTIFSVSVMAQDVTIIYTNDLHAHVDSYKVPYIADGKRDIGGFAN
CCHHHHHHHHHHHHHHHHHHHHHEECCEEEEEECCCEEHHCCCCCCEEECCCCCCCCHHH
ISTLVKQEKAKNKATFYFDTGDYFTGPYISSLTKGEAIIDIMNTMPFDAVSIGNHEFDHG
HHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHCCCHHHHHHHHCCCCCEEECCCCCCCCC
WDNALRQLSKANFPVLLGNVYHKESENPFWNKPYTILEKDGIKIGIIGLHGVFAFNDTVS
HHHHHHHHHHCCCCEEECCEEECCCCCCCCCCCCEEEECCCCEEEEEEEEEEEEECCCHH
ELSLQGLDNDNNNRFDKSSETLKNQGIEARDEVKYLQHYIDELRDKVDLTVALVHEGVPA
HHEEECCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCC
RQSSIGNTDVRRALDKDIQTASKVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDI
HHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEECCCEEEEEECCCCCEE
GKLVLDVNPTARTHKMKSFELKTVYADEWIPDPTTQKVINGWNKKLADIVRQPVGESSIA
EEEEEECCCCHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEE
LTRAYGESSQLGNLFTDAMLVAAPTAQIALINSGSLRADINAGTITFGDITSTFPFKNEL
EEEECCCCCHHHHHHHHHHHEECCCEEEEEEECCCEEEECCCCEEEECCCCCCCCCCCCH
TEMDLSGKDLRNLLEHGASLTNGILQMSKGAEMSYTPQKPVGQRIVSFKINGEEIVDTNI
HHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCEEECCCE
YHVATTTFLALEGDGFLAFKEGKNVQVRAGNNMSDVVIDYLKKGHKITPAQVNEMRVDVS
EEEEEEEEEEEECCCEEEEECCCEEEEEECCCHHHHHHHHHHCCCEECCCCCCEEEEEEC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]