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The map label for this gene is munIM [H]

Identifier: 218701012

GI number: 218701012

Start: 2790862

End: 2791524

Strand: Direct

Name: munIM [H]

Synonym: ECIAI39_2703

Alternate gene names: 218701012

Gene position: 2790862-2791524 (Clockwise)

Preceding gene: 218701011

Following gene: 218701016

Centisome position: 54.38

GC content: 49.62

Gene sequence:

>663_bases
GTGGGGTGGTTTATGACTAAGAAATACACACTAATCTATGCAGATCCACCCTGGGTATACCGGGACAAAGCCGCAGATGG
TAATCGCGGTGCCGGTTTTAAATATCCGGTTATGAGTGTGCTGGATATCTGCCGCCTTCCTGTGTGGGATTTGACCGCTG
AAAACTGTCTGTTGGCCATGTGGTGGGTGCCAACACAACCACTCGAAGCACTAAAAGTTGTTGAAGCCTGGGGATTTCGT
CTGATGACGATGAAGGGCTTCACGTGGATAAAATGTGGTAGTCGACAACCAGATAAACTGGTTATGGGTATGGGACACAT
GACTCGCGCCAATAGTGAAGATTGCCTGTTTGCGGTAAAGGGAAAACTACCTACGCGCATTAATGCAGGGATCGTTCAGT
CATTTACCGCACCGCGGCTTGAGCATTCAAGAAAGCCAGATATCGTTCGTGAAAAACTTGTGCAATTATTAGGCGATGTT
TCTCGCATTGAACTGTTCGCCCGCCAGACGTCTCATGGCTTCGATGTTTGGGGTAATCAGTGCGAAGACCCGGCAGTGCA
ACTACACCCTGGATACGCGTTGGATATTGGCGGATTAACAAATGCATTCAGCAATGCTCCGCTGTCACCAACAGACATCC
AGGGGCGGGAGCGTGCTGCATGA

Upstream 100 bases:

>100_bases
TCTGGTGATGTTGAACGTGCAAGGATCGCGGTTCGCAGGGACCAATGTATCACAAAAGGTAACGGCCTTCGCCGTGGCGA
CTATAACGGCATAGGATGTT

Downstream 100 bases:

>100_bases
ACAGGGCATCACCAGCAGATTTAAGAAAATGCCTTGAAACTGCAAACATGCTTGCACACAGCGGGATCAGGTTTGTTCCA
ATTCCCGCTGTCACTGATGC

Product: putative adenine methylase from phage origin

Products: NA

Alternate protein names: Adenine-specific methyltransferase MunI; M.MunI [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MGWFMTKKYTLIYADPPWVYRDKAADGNRGAGFKYPVMSVLDICRLPVWDLTAENCLLAMWWVPTQPLEALKVVEAWGFR
LMTMKGFTWIKCGSRQPDKLVMGMGHMTRANSEDCLFAVKGKLPTRINAGIVQSFTAPRLEHSRKPDIVREKLVQLLGDV
SRIELFARQTSHGFDVWGNQCEDPAVQLHPGYALDIGGLTNAFSNAPLSPTDIQGRERAA

Sequences:

>Translated_220_residues
MGWFMTKKYTLIYADPPWVYRDKAADGNRGAGFKYPVMSVLDICRLPVWDLTAENCLLAMWWVPTQPLEALKVVEAWGFR
LMTMKGFTWIKCGSRQPDKLVMGMGHMTRANSEDCLFAVKGKLPTRINAGIVQSFTAPRLEHSRKPDIVREKLVQLLGDV
SRIELFARQTSHGFDVWGNQCEDPAVQLHPGYALDIGGLTNAFSNAPLSPTDIQGRERAA
>Mature_219_residues
GWFMTKKYTLIYADPPWVYRDKAADGNRGAGFKYPVMSVLDICRLPVWDLTAENCLLAMWWVPTQPLEALKVVEAWGFRL
MTMKGFTWIKCGSRQPDKLVMGMGHMTRANSEDCLFAVKGKLPTRINAGIVQSFTAPRLEHSRKPDIVREKLVQLLGDVS
RIELFARQTSHGFDVWGNQCEDPAVQLHPGYALDIGGLTNAFSNAPLSPTDIQGRERAA

Specific function: This methylase recognizes the double-stranded sequence CAATTG, causes specific methylation on A-3 on both strands, and protects the DNA from cleavage by the MunI endonuclease [H]

COG id: COG4725

COG function: function code TK; Transcriptional activator, adenine-specific DNA methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MT-A70-like family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR007757 [H]

Pfam domain/function: PF05063 MT-A70 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 24716; Mature: 24585

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS00092 N6_MTASE ; PS51143 MT_A70

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGWFMTKKYTLIYADPPWVYRDKAADGNRGAGFKYPVMSVLDICRLPVWDLTAENCLLAM
CCCEEEEEEEEEEECCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEE
WWVPTQPLEALKVVEAWGFRLMTMKGFTWIKCGSRQPDKLVMGMGHMTRANSEDCLFAVK
EECCCCHHHHHHHHHHHCEEEEEECCEEEEECCCCCCCCEEECCCCCCCCCCCCEEEEEC
GKLPTRINAGIVQSFTAPRLEHSRKPDIVREKLVQLLGDVSRIELFARQTSHGFDVWGNQ
CCCCCCCCCHHHHHHCCCHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
CEDPAVQLHPGYALDIGGLTNAFSNAPLSPTDIQGRERAA
CCCCCEEECCCEEEECCCHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GWFMTKKYTLIYADPPWVYRDKAADGNRGAGFKYPVMSVLDICRLPVWDLTAENCLLAM
CCEEEEEEEEEEECCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEE
WWVPTQPLEALKVVEAWGFRLMTMKGFTWIKCGSRQPDKLVMGMGHMTRANSEDCLFAVK
EECCCCHHHHHHHHHHHCEEEEEECCEEEEECCCCCCCCEEECCCCCCCCCCCCEEEEEC
GKLPTRINAGIVQSFTAPRLEHSRKPDIVREKLVQLLGDVSRIELFARQTSHGFDVWGNQ
CCCCCCCCCHHHHHHCCCHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
CEDPAVQLHPGYALDIGGLTNAFSNAPLSPTDIQGRERAA
CCCCCEEECCCEEEECCCHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8181741 [H]