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The map label for this gene is b0257 [C]

Identifier: 218700963

GI number: 218700963

Start: 2747809

End: 2748291

Strand: Direct

Name: b0257 [C]

Synonym: ECIAI39_2650

Alternate gene names: 218700963

Gene position: 2747809-2748291 (Clockwise)

Preceding gene: 218700962

Following gene: 218700964

Centisome position: 53.54

GC content: 54.87

Gene sequence:

>483_bases
GTGATGCCACTGCTGGATAAGCTGCGTAAGCTGTACGGGGTCGGACCGGTATGCAGTGAACTACATATTGCCCCGTCAAC
GTATTACCACTGTCAGCAACAGCGACATCATCCTGATAAACGCAGTGCCCGTGCGCAGCGCGATGACTGGCTGAAGAAAG
AGATACTGCGCGTATACGATGGGAATCATCAGGTATACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGAATC
AGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGCAC
TACCGTCAGCCGGAAAGCCGTTGCCGCAGGCGACCGCGTAAACCGTCAGTTTGTGGCAGAACGTCCTGACCAGTTGTGGG
TGGCTGATTTTACTTACGGTGTGCCCGGAGTTCAGGGCGGGCATGGATGCTTAAATGAACCGCGAGTCTGTCTGGAATAT
TGA

Upstream 100 bases:

>100_bases
AGCTGGAACGTGAAAATCGTGAACTGCGCCGCAGTAACGATATCCTTCGCCAGGCTTCCGCTTATTTTGCGAAGGCGGAG
TTCGACCGCCTCTGGAAAAA

Downstream 100 bases:

>100_bases
ACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGCGGCAT
GCCCGCAGGGTGATGTAACC

Product: transposase ORF B (fragment), IS629

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MMPLLDKLRKLYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLKKEILRVYDGNHQVYGVRKVWRQLLREGI
RVARCTVARLMAVMGLAGVLRGKKVRTTVSRKAVAAGDRVNRQFVAERPDQLWVADFTYGVPGVQGGHGCLNEPRVCLEY

Sequences:

>Translated_160_residues
MMPLLDKLRKLYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLKKEILRVYDGNHQVYGVRKVWRQLLREGI
RVARCTVARLMAVMGLAGVLRGKKVRTTVSRKAVAAGDRVNRQFVAERPDQLWVADFTYGVPGVQGGHGCLNEPRVCLEY
>Mature_160_residues
MMPLLDKLRKLYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLKKEILRVYDGNHQVYGVRKVWRQLLREGI
RVARCTVARLMAVMGLAGVLRGKKVRTTVSRKAVAAGDRVNRQFVAERPDQLWVADFTYGVPGVQGGHGCLNEPRVCLEY

Specific function: Involved in the transposition of the insertion sequence [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=140, Percent_Identity=45, Blast_Score=119, Evalue=1e-27,
Organism=Escherichia coli, GI1789981, Length=143, Percent_Identity=30.7692307692308, Blast_Score=68, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 18295; Mature: 18295

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPLLDKLRKLYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLKKEILRVYD
CCHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
GNHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTVSRKAVAAGDRV
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHH
NRQFVAERPDQLWVADFTYGVPGVQGGHGCLNEPRVCLEY
HHHHHHCCCCCEEEEHCCCCCCCCCCCCCCCCCCCEECCC
>Mature Secondary Structure
MMPLLDKLRKLYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLKKEILRVYD
CCHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
GNHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTVSRKAVAAGDRV
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHH
NRQFVAERPDQLWVADFTYGVPGVQGGHGCLNEPRVCLEY
HHHHHHCCCCCEEEEHCCCCCCCCCCCCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2159625; 2824781 [H]