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The map label for this gene is hyfB

Identifier: 218700940

GI number: 218700940

Start: 2718542

End: 2720560

Strand: Direct

Name: hyfB

Synonym: ECIAI39_2621

Alternate gene names: 218700940

Gene position: 2718542-2720560 (Clockwise)

Preceding gene: 218700939

Following gene: 218700941

Centisome position: 52.97

GC content: 55.42

Gene sequence:

>2019_bases
ATGGATGCCCTGCAATTATTAACCTGGTCGCTGATCCTCTATCTGTTTGCCAGTCTGGCTTCGCTGTTTTTACTCGGTCT
GGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAAT
TACATGCAGGCGTAACTTTAGTCGCCCGTTTTGCCACACCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTC
TCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCGCTCTATTCATTGACTTATATGCGCGAATACGA
GGGTAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATCTTTTCATCGCATCGATGGTTGTCCTGCTGGTGATGGACAACG
CTTTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCG
ATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATTATGATCGCCTTCTTGCTGATGGGGCG
CGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGG
CCTTCTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTTGCCGCGCGCTCACCCTGCCGCACCATCG
CACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATCGGTATTTTCGGCATCCTTAAAGTAGCGATGGATCTGCTGGC
GCAAACGGGTTTGCCGCTGTGGTGGGGCATTCTGGTGATGGCGATCGGCGCAATCTCCGCGCTCCTGGGCGTGCTGTATG
CGCTGGCGGAACAGGATATCAAACGGCTGCTGGCCTGGAGTACCGTCGAAAACGTCGGCATTATTTTGCTGGCGGTCGGT
GTGGCGATGGTCGGTCTGTCACTGCACGACCCGCTGCTCACCGTTGTTGGACTGCTCGGCGCGCTGTTTCATCTGCTCAA
CCATGCGCTGTTCAAAGGGCTGCTGTTTCTCGGCGCGGGTGCGATTATTTCGCGTTTGCATACCCACGACATGGAAAAAA
TGGGGGCACTGGCGAAACGTATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCG
CTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACT
TGCGGGGCCTATTGCTATGGTGATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTCAAAATGTACGGTATTA
CTTTCTGTGGAGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGACGTGCCAAATACGATGATCGTCGCCATGCTACTG
CTCGCGGCACTCTGCGTATTCATTGCGCTTAGTGCCAGTTGGCTGGCACCGAAGATAATGCACATTGCCCATGCGTTTAC
CAATACCCCTCCCGTCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACGGGTCACTCCCTCATTAC
TGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCGTTTCGT
CGCACAGGAGATGCCTGGGCATGCGGCTACGGCTGGGATAATGCGATGGCCCCGTCAGGCAATGGCGTGATGCAGCCGCT
GCGTGTGGTCTTTTGTGCGCTATTTCGTCTACGACAACAGCTCGACCCTACGCTGAGGCTGACCAAAGGTCTTGCGCACG
TCACCGCCAGGGCTCAGAGCACAGAACCCTTCTGGGATGAGCGGGTGATCCGCCCCATCGTGAGCGCCACCCAACGGCTG
GCCAAAGAAATACAGCATCTGCAAAGCGGCGACTTTCGTCTCTATTGCCTGTATGTGGTCGCCGCACTGGTTGTGCTGCT
AATCGCTATTGCCGTCTAA

Upstream 100 bases:

>100_bases
ATAGCCTGCAACGCCAGATGAAAGAAAAACAGCGCCTTGCCGCAAGCTGGTTTGCCAATGGCGGGGAGGCTCCCCTTTCC
CTCACTCAGGAGCAACGCTA

Downstream 100 bases:

>100_bases
GGAAATCACCATGAGACAAACTCTTTGCGACGGATATCTGGTCATTTTTGCGTTAGCACAGGCCGTGATTCTGCTGGTGC
TAACCCCGCTTTTTACGGGG

Product: hydrogenase 4 subunit B

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 672; Mature: 672

Protein sequence:

>672_residues
MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGVIGIISGITQLHAGVTLVARFATPFEFADLTLRMDSL
SAFMVLVISLLVVVCSLYSLTYMREYEGKGAAAMGFFMNLFIASMVVLLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGFGAKAGMMPLHSWLPRAHPAAPS
HASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWGILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVG
VAMVGLSLHDPLLTVVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGCLAISAIPP
LNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKMYGITFCGAPRSTHAEEAQDVPNTMIVAMLL
LAALCVFIALSASWLAPKIMHIAHAFTNTPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFR
RTGDAWACGYGWDNAMAPSGNGVMQPLRVVFCALFRLRQQLDPTLRLTKGLAHVTARAQSTEPFWDERVIRPIVSATQRL
AKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV

Sequences:

>Translated_672_residues
MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGVIGIISGITQLHAGVTLVARFATPFEFADLTLRMDSL
SAFMVLVISLLVVVCSLYSLTYMREYEGKGAAAMGFFMNLFIASMVVLLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGFGAKAGMMPLHSWLPRAHPAAPS
HASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWGILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVG
VAMVGLSLHDPLLTVVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGCLAISAIPP
LNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKMYGITFCGAPRSTHAEEAQDVPNTMIVAMLL
LAALCVFIALSASWLAPKIMHIAHAFTNTPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFR
RTGDAWACGYGWDNAMAPSGNGVMQPLRVVFCALFRLRQQLDPTLRLTKGLAHVTARAQSTEPFWDERVIRPIVSATQRL
AKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV
>Mature_672_residues
MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGVIGIISGITQLHAGVTLVARFATPFEFADLTLRMDSL
SAFMVLVISLLVVVCSLYSLTYMREYEGKGAAAMGFFMNLFIASMVVLLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGFGAKAGMMPLHSWLPRAHPAAPS
HASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWGILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVG
VAMVGLSLHDPLLTVVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGCLAISAIPP
LNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKMYGITFCGAPRSTHAEEAQDVPNTMIVAMLL
LAALCVFIALSASWLAPKIMHIAHAFTNTPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFR
RTGDAWACGYGWDNAMAPSGNGVMQPLRVVFCALFRLRQQLDPTLRLTKGLAHVTARAQSTEPFWDERVIRPIVSATQRL
AKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=424, Percent_Identity=28.7735849056604, Blast_Score=116, Evalue=8e-26,
Organism=Homo sapiens, GI251831116, Length=333, Percent_Identity=24.024024024024, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1788827, Length=672, Percent_Identity=98.5119047619048, Blast_Score=1315, Evalue=0.0,
Organism=Escherichia coli, GI2367154, Length=608, Percent_Identity=36.0197368421053, Blast_Score=328, Evalue=8e-91,
Organism=Escherichia coli, GI1788831, Length=357, Percent_Identity=31.3725490196078, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1788829, Length=435, Percent_Identity=24.8275862068966, Blast_Score=127, Evalue=3e-30,
Organism=Escherichia coli, GI1788613, Length=349, Percent_Identity=27.2206303724928, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1788614, Length=524, Percent_Identity=28.4351145038168, Blast_Score=124, Evalue=3e-29,
Organism=Escherichia coli, GI145693160, Length=339, Percent_Identity=27.4336283185841, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 72681; Mature: 72681

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGVIGIISGITQLHAGVTL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTYMREYEGKGAAAMGFFMNL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
FIASMVVLLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTSINAGMLYFFIAHAGSVLIMI
HHHHHHHHHHHCCHHHHHHHHHHHHCCCHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
AFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGFGAKAGMMPLHSWLPRAHPAAPS
HHHHHCCCCCCCCHHHHEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCH
HASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWGILVMAIGAISALLGVLYALAEQDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLTVVGLLGALFHLLNHALFKGLLFLGAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIISRLHTHDMEKMGALAKRMPWTAAACLIGCLAISAIPPLNGFISEWYTWQSLFSLSRV
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
EAVALQLAGPIAMVMLAVTGGLAVMCFVKMYGITFCGAPRSTHAEEAQDVPNTMIVAMLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHCCCHHHHHHHHH
LAALCVFIALSASWLAPKIMHIAHAFTNTPPVTVASGIALVPGTFHTRVTPSLLLLLLLA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCEECCCCCCCCCCHHHHHHHHHH
MPLLPGLYWLWCRSRRAAFRRTGDAWACGYGWDNAMAPSGNGVMQPLRVVFCALFRLRQQ
HHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LDPTLRLTKGLAHVTARAQSTEPFWDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVV
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHH
AALVVLLIAIAV
HHHHHHHHHHCC
>Mature Secondary Structure
MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGVIGIISGITQLHAGVTL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTYMREYEGKGAAAMGFFMNL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
FIASMVVLLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTSINAGMLYFFIAHAGSVLIMI
HHHHHHHHHHHCCHHHHHHHHHHHHCCCHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
AFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGFGAKAGMMPLHSWLPRAHPAAPS
HHHHHCCCCCCCCHHHHEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCH
HASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWGILVMAIGAISALLGVLYALAEQDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLTVVGLLGALFHLLNHALFKGLLFLGAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIISRLHTHDMEKMGALAKRMPWTAAACLIGCLAISAIPPLNGFISEWYTWQSLFSLSRV
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
EAVALQLAGPIAMVMLAVTGGLAVMCFVKMYGITFCGAPRSTHAEEAQDVPNTMIVAMLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHCCCHHHHHHHHH
LAALCVFIALSASWLAPKIMHIAHAFTNTPPVTVASGIALVPGTFHTRVTPSLLLLLLLA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCEECCCCCCCCCCHHHHHHHHHH
MPLLPGLYWLWCRSRRAAFRRTGDAWACGYGWDNAMAPSGNGVMQPLRVVFCALFRLRQQ
HHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LDPTLRLTKGLAHVTARAQSTEPFWDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVV
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHH
AALVVLLIAIAV
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2016588 [H]