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The map label for this gene is nupC

Identifier: 218700862

GI number: 218700862

Start: 2636203

End: 2637405

Strand: Direct

Name: nupC

Synonym: ECIAI39_2539

Alternate gene names: 218700862

Gene position: 2636203-2637405 (Clockwise)

Preceding gene: 218700860

Following gene: 218700864

Centisome position: 51.37

GC content: 49.71

Gene sequence:

>1203_bases
ATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGCGATTCTCGCACTGCTGGTAAGCAGCGACCGCAAAAA
AATTCGTATCCGTTATGTTATTCAACTGCTTGTTATCGAAGTGTTACTGGCGTGGTTCTTCCTGAACTCCGACGTTGGTT
TGGGCTTCGTGAAAGGCTTCTCCGAAATGTTTGAGAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGT
AGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCGCTGATCGGTAT
TCTCCAGCACATTCGCGTGTTGCCAGTGATTATCCGCGCAATTGGTTTCCTGCTTTCCAAAGTCAACGGTATGGGCAAAC
TGGAATCCTTTAACGCCGTCAGCTCTCTGATTCTGGGTCAGTCTGAAAACTTTATTGCCTATAAAGATATCCTCGGCAAA
ATCTCCCGCAATCGTATGTACACCATGGCAGCTACGGCGATGTCCACCGTGTCGATGTCCATCGTTGGTGCATACATGAC
CATGCTGGAGCCGAAATATGTCGTTGCGGCGCTGGTACTGAACATGTTCAGCACCTTTATCGTGCTGTCGCTGATCAACC
CTTACCGTGTTGATGCCAGTGAAGAAAACATTCAGATGTCCAACCTGCACGAAGGTCAGAGCTTCTTCGAAATGCTGGGT
GAATACATTCTGGCAGGTTTCAAAGTTGCCATTATCGTTGCCGCAATGCTGATCGGCTTTATCGCCCTGATCGCCGCGCT
GAACGCACTGTTTGCTACCGTGACTGGCTGGTTTGGCTACAGCATCTCCTTCCAGGGCATCCTGGGCTACATCTTCTATC
CGATTGCATGGGTGATGGGTGTTCCTTCCAGTGAAGCACTGCAAGTGGGCAGTATCATGGCGACCAAACTGGTTTCCAAC
GAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGTTCCT
GGTTTCCTTCGCTAACTTCTCATCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGG
TTTCTCGCTTCGGTCTGAAACTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTG
TAA

Upstream 100 bases:

>100_bases
TCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCT
GCCTCATATTTGGAGCAAAT

Downstream 100 bases:

>100_bases
GACCATACATAAAAAAGCCGGGGATAATTCCCCGGTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCA
AATACCCTTGCAGATATTGC

Product: nucleoside (except guanosine) transporter

Products: uridine [Cytoplasm]; thymidine [Cytoplasm]; inosine [Cytoplasm]; Deoxyuridine [Cytoplasm]; deoxyinosine [Cytoplasm]; deoxycytidine [Cytoplasm]; deoxyadenosine [Cytoplasm]; cytidine [Cytoplasm]; Proton [Cytoplasm]; adenosine [Cytoplasm] [C]

Alternate protein names: Nucleoside-transport system protein nupC

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLAWFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFG
SMNDQGLAFFFLKVLCPIVFISALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVDASEENIQMSNLHEGQSFFEMLG
EYILAGFKVAIIVAAMLIGFIALIAALNALFATVTGWFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSN
EFVAMMDLQKIASTLSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLSASIAALVL

Sequences:

>Translated_400_residues
MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLAWFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFG
SMNDQGLAFFFLKVLCPIVFISALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVDASEENIQMSNLHEGQSFFEMLG
EYILAGFKVAIIVAAMLIGFIALIAALNALFATVTGWFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSN
EFVAMMDLQKIASTLSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLSASIAALVL
>Mature_400_residues
MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLAWFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFG
SMNDQGLAFFFLKVLCPIVFISALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVDASEENIQMSNLHEGQSFFEMLG
EYILAGFKVAIIVAAMLIGFIALIAALNALFATVTGWFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSN
EFVAMMDLQKIASTLSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLSASIAALVL

Specific function: Transports nucleosides with a high affinity except guanosine and deoxyguanosine. Driven by a proton motive force

COG id: COG1972

COG function: function code F; Nucleoside permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family

Homologues:

Organism=Homo sapiens, GI42542381, Length=395, Percent_Identity=25.8227848101266, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI11545853, Length=373, Percent_Identity=24.1286863270777, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI227116277, Length=403, Percent_Identity=22.0843672456576, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1788737, Length=400, Percent_Identity=100, Blast_Score=787, Evalue=0.0,
Organism=Escherichia coli, GI1788485, Length=414, Percent_Identity=32.1256038647343, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1788488, Length=413, Percent_Identity=30.5084745762712, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17560276, Length=393, Percent_Identity=24.4274809160305, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71991794, Length=392, Percent_Identity=25.2551020408163, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI45552519, Length=391, Percent_Identity=25.8312020460358, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI45552517, Length=391, Percent_Identity=25.8312020460358, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI19921868, Length=391, Percent_Identity=25.8312020460358, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI281360430, Length=344, Percent_Identity=24.7093023255814, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUPC_ECOL6 (P0AFF3)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_754814.1
- ProteinModelPortal:   P0AFF3
- EnsemblBacteria:   EBESCT00000041536
- GeneID:   1038532
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c2932
- GeneTree:   EBGT00050000009760
- HOGENOM:   HBG713614
- OMA:   KINGMGK
- ProtClustDB:   CLSK880382
- InterPro:   IPR008276
- InterPro:   IPR018270
- InterPro:   IPR011657
- InterPro:   IPR002668
- PANTHER:   PTHR10590
- TIGRFAMs:   TIGR00804

Pfam domain/function: PF07662 Nucleos_tra2_C; PF01773 Nucleos_tra2_N

EC number: NA

Molecular weight: Translated: 43476; Mature: 43476

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x23aaa22c)-; HASH(0x23a405a0)-; HASH(0x22462558)-; HASH(0x23498778)-; HASH(0x20f419e0)-; HASH(0x23cd635c)-; HASH(0x238f5fb0)-; HASH(0x237f32c0)-; HASH(0x23b635e4)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLAWFFLNSDVGLGFVKGF
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
SEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFISALIGILQHIRVLPVIIRA
HHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGKISRNRMYTMAATAMSTVSMS
HHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
IVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVDASEENIQMSNLHEGQSFFEMLG
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCHHHHHHHHH
EYILAGFKVAIIVAAMLIGFIALIAALNALFATVTGWFGYSISFQGILGYIFYPIAWVMG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHC
VPSSEALQVGSIMATKLVSNEFVAMMDLQKIASTLSPRAEGIISVFLVSFANFSSIGIIA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHH
GAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLSASIAALVL
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLAWFFLNSDVGLGFVKGF
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
SEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFISALIGILQHIRVLPVIIRA
HHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGKISRNRMYTMAATAMSTVSMS
HHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
IVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVDASEENIQMSNLHEGQSFFEMLG
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCHHHHHHHHH
EYILAGFKVAIIVAAMLIGFIALIAALNALFATVTGWFGYSISFQGILGYIFYPIAWVMG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHC
VPSSEALQVGSIMATKLVSNEFVAMMDLQKIASTLSPRAEGIISVFLVSFANFSSIGIIA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHH
GAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLSASIAALVL
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine [Periplasm]; thymidine [Periplasm]; inosine [Periplasm]; Deoxyuridine [Periplasm]; deoxyinosine [Periplasm]; deoxycytidine [Periplasm]; deoxyadenosine [Periplasm]; cytidine [Periplasm]; Proton [Periplasm]; adenosine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uridine [Periplasm] = Proton [Cytoplasm] + uridine [Cytoplasm] Proton [Periplasm] + thymidine [Periplasm] = Proton [Cytoplasm] + thymidine [Cytoplasm] Proton [Periplasm] + inosine [Periplasm] = Proton [Cytoplasm] + inosine [Cytoplasm]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157