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The map label for this gene is insK [H]

Identifier: 218700860

GI number: 218700860

Start: 2633771

End: 2634493

Strand: Direct

Name: insK [H]

Synonym: ECIAI39_2537

Alternate gene names: 218700860

Gene position: 2633771-2634493 (Clockwise)

Preceding gene: 218700859

Following gene: 218700862

Centisome position: 51.32

GC content: 48.41

Gene sequence:

>723_bases
GTGAAACAGCTCATAGCCTCCATCTTCCATGAACACCGGGGATGTTATGGGTACCGGCGGATCCATTGTGAGCTTCAGAA
AAGAGGTCTTAAGTTCAGTGGCAAAACAGTGCGAAAACTGATGCAACAGCTCGGCCTGAAGTCTCCTGTGCGCCTGAAGA
AATACCGGTCTTACCGGGGAAATATGGGACTCGCTGCAGAAAATATCCTTCAGCGGCAGTTCAAAGCGGAAGCTCCCTGT
GAGAAATGGGTGACAGATATCACCGAATTCAGGGCTGGCGGACAAAAACTGTACCTGTCCCCGATACTTGATTTGTTTAA
CGGGGAAATCGTGGCGTGGGAAACTGCCTGCAGGCCTACAGAGGAGCTGGTGAAGCGCATGTTAAATAAGGGGCTGGAAA
GCCTTGCAGAAGGAGAAAAACCGCTGCTCCACAGTGATCAAGGGTGGCATTACCGGATAAAAAGTTATCAGTCTGCCCTG
GCAGACAAAGGGTTAGTGCAGAGCATGTCGCGCAAGGGCAACTGTCTTGATAATGCGGTAATGGAGAATTTCTTTGGTCA
CCTGAAAGAAGAAATGTACTATCGCCGGGACTACAGAAGTGTAGAAGAGCTGGAAAATGCCGTTAACGAATACATAACTT
ACTGGAATCAAAAAAGAATAAAACTCAGTCTTGGGGGACTCAGCCCGGTAGAATACCGGACTGAGTATCAAAAAGCCGGT
TAA

Upstream 100 bases:

>100_bases
CGGACACTGCCAGTCTGACCTGTTAAAGGCTGCAGGGCTGGCCAGAAGCACGCTGTACTACCAGCTGTCACTGCAGAAGG
CTAAGGACAAGTATGCTGAT

Downstream 100 bases:

>100_bases
CTGAAACTGTCCAAGTTTTGGGGTTCAGTTCATAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCG
GATAAGGCGTTTTACGCCGC

Product: transposase ORF B, IS3 family, IS150 group

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MKQLIASIFHEHRGCYGYRRIHCELQKRGLKFSGKTVRKLMQQLGLKSPVRLKKYRSYRGNMGLAAENILQRQFKAEAPC
EKWVTDITEFRAGGQKLYLSPILDLFNGEIVAWETACRPTEELVKRMLNKGLESLAEGEKPLLHSDQGWHYRIKSYQSAL
ADKGLVQSMSRKGNCLDNAVMENFFGHLKEEMYYRRDYRSVEELENAVNEYITYWNQKRIKLSLGGLSPVEYRTEYQKAG

Sequences:

>Translated_240_residues
MKQLIASIFHEHRGCYGYRRIHCELQKRGLKFSGKTVRKLMQQLGLKSPVRLKKYRSYRGNMGLAAENILQRQFKAEAPC
EKWVTDITEFRAGGQKLYLSPILDLFNGEIVAWETACRPTEELVKRMLNKGLESLAEGEKPLLHSDQGWHYRIKSYQSAL
ADKGLVQSMSRKGNCLDNAVMENFFGHLKEEMYYRRDYRSVEELENAVNEYITYWNQKRIKLSLGGLSPVEYRTEYQKAG
>Mature_240_residues
MKQLIASIFHEHRGCYGYRRIHCELQKRGLKFSGKTVRKLMQQLGLKSPVRLKKYRSYRGNMGLAAENILQRQFKAEAPC
EKWVTDITEFRAGGQKLYLSPILDLFNGEIVAWETACRPTEELVKRMLNKGLESLAEGEKPLLHSDQGWHYRIKSYQSAL
ADKGLVQSMSRKGNCLDNAVMENFFGHLKEEMYYRRDYRSVEELENAVNEYITYWNQKRIKLSLGGLSPVEYRTEYQKAG

Specific function: Involved in the transposition of the insertion sequence IS150 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=207, Percent_Identity=28.5024154589372, Blast_Score=92, Evalue=6e-19,
Organism=Escherichia coli, GI1789981, Length=235, Percent_Identity=51.4893617021277, Blast_Score=271, Evalue=2e-74,
Organism=Escherichia coli, GI1788405, Length=231, Percent_Identity=32.4675324675325, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1787263, Length=231, Percent_Identity=32.4675324675325, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1786753, Length=231, Percent_Identity=32.4675324675325, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1786570, Length=231, Percent_Identity=32.4675324675325, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1786490, Length=231, Percent_Identity=32.4675324675325, Blast_Score=114, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 27971; Mature: 27971

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLIASIFHEHRGCYGYRRIHCELQKRGLKFSGKTVRKLMQQLGLKSPVRLKKYRSYRG
CHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCC
NMGLAAENILQRQFKAEAPCEKWVTDITEFRAGGQKLYLSPILDLFNGEIVAWETACRPT
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCEEEEECCCCCH
EELVKRMLNKGLESLAEGEKPLLHSDQGWHYRIKSYQSALADKGLVQSMSRKGNCLDNAV
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
MENFFGHLKEEMYYRRDYRSVEELENAVNEYITYWNQKRIKLSLGGLSPVEYRTEYQKAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCC
>Mature Secondary Structure
MKQLIASIFHEHRGCYGYRRIHCELQKRGLKFSGKTVRKLMQQLGLKSPVRLKKYRSYRG
CHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCC
NMGLAAENILQRQFKAEAPCEKWVTDITEFRAGGQKLYLSPILDLFNGEIVAWETACRPT
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCEEEEECCCCCH
EELVKRMLNKGLESLAEGEKPLLHSDQGWHYRIKSYQSALADKGLVQSMSRKGNCLDNAV
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
MENFFGHLKEEMYYRRDYRSVEELENAVNEYITYWNQKRIKLSLGGLSPVEYRTEYQKAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2841644; 2541046; 8041620; 9278503 [H]