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The map label for this gene is elaD

Identifier: 218700744

GI number: 218700744

Start: 2501729

End: 2502937

Strand: Direct

Name: elaD

Synonym: ECIAI39_2417

Alternate gene names: 218700744

Gene position: 2501729-2502937 (Clockwise)

Preceding gene: 218700743

Following gene: 218700748

Centisome position: 48.75

GC content: 34.57

Gene sequence:

>1209_bases
ATGGTTACAGTTGTCAGCAATTATTGTCAATTATCTCAAACCCAACTCAGTCAGACATTTGCAGAAAAATTTACTGTGAC
CGATGAATTACTGCAGTCTTTAAAAAAAACAGCGTTATCCGGAGATGAAGAAAGTATTGAGTTACTGCATAACATTGCGT
TAGGTTATGATGAATTTGGGAAAAAAGCTGAAGATATTCTTTACCATATCGTTAGAAACCCAACAAATGAGACCCTATCG
ATTATCCGACTTATTAAAAATGCCTGTTTAAAATTATATAATTTGGCACATACCGCAACCAACCCCCCCCTCAAATCACA
TGATTCAGATGATCTCCTGTTCAAAAAACTATTCTCCCCTTCGAAATTAATGACAATTATCGGTGAGGACATTCCTCTTA
TATCAGAAAAACAGTCGCTTTCAAAGGTACTTTTAAATGATAAGAATAATGAACTGAGCGATGGGACAAACTTCTGGGAT
AAAAATCGTCAATTAACCACGGATGAAATAGATTGCTATCTTCAGAAGATCGCCGCTAATGCAAAAAATACTCAAGTCAA
TTATCCTACTGATTTCTACCTCCCCGATTCCAACAGCACTTACCTGGAAATCGCTCTCAATGATAATATTAAGAGCGATC
CATCATGGCCGAAAGAAGTCCAGTTATTCCCCATAAATACTGGCGGACACTGGATATTAGTTTCGCTACAAAAAATAGTT
AATAAAAAAAATAACACACAACAAATAAAATGCATCATATTCAATTCATTACGTGCACTAGGCCATGAAAAAGAAAATTC
ACTTAAGCGCATCATTAATAGTTTTAATTCTGAACTCATGGGAGAAATGTCGAATAATAATATAAAAGTTCATTTAACTA
AACAAGAGATAACATTTTTACATGCCGATCTTCAGCAATACTTAAGCCAAAGTTGCGGTGCATTTGTGTGCATGGCAGCC
AAAGAAGTAATTGAACAAAGGGAAAGCAATTCTGACAGCGCTCCCTATACGTTATTAAAAAACTATGCTGACAGATTTAA
AAAATATTCAGCAGAAGAGCAGTACGAAATTGATTTTCAACATCGACTGATAAACAGAAATTGTTATTTAGATAAATATG
GCGATGCAAATATCAATGATTATTATAGAGACTTAGAAATAAAACACTCACAACCCCAAAATAGAGCATCAGGCAAAAGA
GTAAGTTAA

Upstream 100 bases:

>100_bases
TTTGATATTTATTCATCTCCTTACGCACTATATTCAGAGCAATAAGTTGCCTCTTTTTATCTTCATTCAGCACAACCTTT
GCAACTAAAAGGAGCCTATA

Downstream 100 bases:

>100_bases
CATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAAAAGTCATCACTGACTG
ATGTCAGTCACACCACCCGC

Product: deubiquitinase

Products: NA

Alternate protein names: Deubiquitinase; Deubiquitinating enzyme; DUB; Deubiquitinating protease [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MVTVVSNYCQLSQTQLSQTFAEKFTVTDELLQSLKKTALSGDEESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLS
IIRLIKNACLKLYNLAHTATNPPLKSHDSDDLLFKKLFSPSKLMTIIGEDIPLISEKQSLSKVLLNDKNNELSDGTNFWD
KNRQLTTDEIDCYLQKIAANAKNTQVNYPTDFYLPDSNSTYLEIALNDNIKSDPSWPKEVQLFPINTGGHWILVSLQKIV
NKKNNTQQIKCIIFNSLRALGHEKENSLKRIINSFNSELMGEMSNNNIKVHLTKQEITFLHADLQQYLSQSCGAFVCMAA
KEVIEQRESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLINRNCYLDKYGDANINDYYRDLEIKHSQPQNRASGKR
VS

Sequences:

>Translated_402_residues
MVTVVSNYCQLSQTQLSQTFAEKFTVTDELLQSLKKTALSGDEESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLS
IIRLIKNACLKLYNLAHTATNPPLKSHDSDDLLFKKLFSPSKLMTIIGEDIPLISEKQSLSKVLLNDKNNELSDGTNFWD
KNRQLTTDEIDCYLQKIAANAKNTQVNYPTDFYLPDSNSTYLEIALNDNIKSDPSWPKEVQLFPINTGGHWILVSLQKIV
NKKNNTQQIKCIIFNSLRALGHEKENSLKRIINSFNSELMGEMSNNNIKVHLTKQEITFLHADLQQYLSQSCGAFVCMAA
KEVIEQRESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLINRNCYLDKYGDANINDYYRDLEIKHSQPQNRASGKR
VS
>Mature_402_residues
MVTVVSNYCQLSQTQLSQTFAEKFTVTDELLQSLKKTALSGDEESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLS
IIRLIKNACLKLYNLAHTATNPPLKSHDSDDLLFKKLFSPSKLMTIIGEDIPLISEKQSLSKVLLNDKNNELSDGTNFWD
KNRQLTTDEIDCYLQKIAANAKNTQVNYPTDFYLPDSNSTYLEIALNDNIKSDPSWPKEVQLFPINTGGHWILVSLQKIV
NKKNNTQQIKCIIFNSLRALGHEKENSLKRIINSFNSELMGEMSNNNIKVHLTKQEITFLHADLQQYLSQSCGAFVCMAA
KEVIEQRESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLINRNCYLDKYGDANINDYYRDLEIKHSQPQNRASGKR
VS

Specific function: Protease that can act as an efficient and specific deubiquitinating enzyme in vitro. Does not possess desumoylating and deneddylating activities. The physiological substrate is unknown [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase C79 family [H]

Homologues:

Organism=Escherichia coli, GI1788604, Length=402, Percent_Identity=91.044776119403, Blast_Score=757, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003653 [H]

Pfam domain/function: PF02902 Peptidase_C48 [H]

EC number: NA

Molecular weight: Translated: 46070; Mature: 46070

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTVVSNYCQLSQTQLSQTFAEKFTVTDELLQSLKKTALSGDEESIELLHNIALGYDEFG
CEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHC
KKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATNPPLKSHDSDDLLFKKLFSP
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCC
SKLMTIIGEDIPLISEKQSLSKVLLNDKNNELSDGTNFWDKNRQLTTDEIDCYLQKIAAN
HHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHCC
AKNTQVNYPTDFYLPDSNSTYLEIALNDNIKSDPSWPKEVQLFPINTGGHWILVSLQKIV
CCCCCCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCEEEEEHHHHH
NKKNNTQQIKCIIFNSLRALGHEKENSLKRIINSFNSELMGEMSNNNIKVHLTKQEITFL
CCCCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHH
HADLQQYLSQSCGAFVCMAAKEVIEQRESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEHHHH
HRLINRNCYLDKYGDANINDYYRDLEIKHSQPQNRASGKRVS
HHHHCCCCCEECCCCCCHHHHHHCCEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MVTVVSNYCQLSQTQLSQTFAEKFTVTDELLQSLKKTALSGDEESIELLHNIALGYDEFG
CEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHC
KKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATNPPLKSHDSDDLLFKKLFSP
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCC
SKLMTIIGEDIPLISEKQSLSKVLLNDKNNELSDGTNFWDKNRQLTTDEIDCYLQKIAAN
HHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHCC
AKNTQVNYPTDFYLPDSNSTYLEIALNDNIKSDPSWPKEVQLFPINTGGHWILVSLQKIV
CCCCCCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCEEEEEHHHHH
NKKNNTQQIKCIIFNSLRALGHEKENSLKRIINSFNSELMGEMSNNNIKVHLTKQEITFL
CCCCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHH
HADLQQYLSQSCGAFVCMAAKEVIEQRESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEHHHH
HRLINRNCYLDKYGDANINDYYRDLEIKHSQPQNRASGKRVS
HHHHCCCCCEECCCCCCHHHHHHCCEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]