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The map label for this gene is yfbF

Identifier: 218700728

GI number: 218700728

Start: 2486085

End: 2487053

Strand: Direct

Name: yfbF

Synonym: ECIAI39_2401

Alternate gene names: 218700728

Gene position: 2486085-2487053 (Clockwise)

Preceding gene: 218700727

Following gene: 218700729

Centisome position: 48.44

GC content: 50.67

Gene sequence:

>969_bases
ATGTTTGAAATCCACCCTGTTAAGAAAGTCTCGGTGGTTATTCCCGTTTATAACGAGCAGGAAAGCTTACCGGAATTAAT
CAGGCGCACCACAAAAGCCTGTGAATCACTGGGGAAAGAGTATGAGATCCTGCTGATTGATGACGGCAGTAGCGATAATT
CCGCGCATATGCTGGTCGATGCCTCACAAGCAGAGGGCAGCCACATCGTGTCTATTTTGCTTAATCGTAATTACGGACAG
CATTCGGCGATCATGGCAGGTTTCAGCCACGTTACCGGCGACTTAATTATTACCCTCGATGCCGATCTCCAGAATCCGCC
AGAGGAAATCCCCCGTCTGGTGGCAAAAGCCGATGAAGGTTACGACGTGGTAGGGACTGTACGCCAGAACCGCCAGGACA
GCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGACTATGGT
TGTATGCTGCGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCT
GGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTGCACCATGCCGAGCGTGAGTTTGGTGAATCCAAATACAGCT
TTATGCGCCTGATTAATTTGATGTACGACCTGGTGACCTGTCTTACCACAACGCCGCTACGTATGCTGAGTCTGCTCGGC
AGCATTATTGCGATTGGCGGTTTTAGCATTGCGGTGCTGCTGGTGATTTTACGCCTGACCTTCGGACCCCAATGGGCGGC
AGAAGGCGTCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTGCTCGGTGAAT
ATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAA
AATGAATAA

Upstream 100 bases:

>100_bases
TAGCGAACGCATCTGTTCGTTGCCGCTGTTCCCGGATATGACCACCGCCGATGCCGACCGCGTCATCACAGCCCTTCAGC
AACTCGCAGGACAATAAGCC

Downstream 100 bases:

>100_bases
TGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCCTGCTGGCTGCCGGTTACGAAATTAGC
GCCATTTTTACCCATACTGA

Product: undecaprenyl phosphate 4-deoxy-4-formamido-L-arabinose transferase

Products: NA

Alternate protein names: Undecaprenyl-phosphate Ara4FN transferase; Ara4FN transferase [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MFEIHPVKKVSVVIPVYNEQESLPELIRRTTKACESLGKEYEILLIDDGSSDNSAHMLVDASQAEGSHIVSILLNRNYGQ
HSAIMAGFSHVTGDLIITLDADLQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLINLMYDLVTCLTTTPLRMLSLLG
SIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAVLFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKE
NE

Sequences:

>Translated_322_residues
MFEIHPVKKVSVVIPVYNEQESLPELIRRTTKACESLGKEYEILLIDDGSSDNSAHMLVDASQAEGSHIVSILLNRNYGQ
HSAIMAGFSHVTGDLIITLDADLQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLINLMYDLVTCLTTTPLRMLSLLG
SIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAVLFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKE
NE
>Mature_322_residues
MFEIHPVKKVSVVIPVYNEQESLPELIRRTTKACESLGKEYEILLIDDGSSDNSAHMLVDASQAEGSHIVSILLNRNYGQ
HSAIMAGFSHVTGDLIITLDADLQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLINLMYDLVTCLTTTPLRMLSLLG
SIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAVLFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKE
NE

Specific function: Catalyzes the transfer of 4-deoxy-4-formamido-L- arabinose from UDP to undecaprenyl phosphate. The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides [H]

COG id: COG0463

COG function: function code M; Glycosyltransferases involved in cell wall biogenesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Homo sapiens, GI4503363, Length=237, Percent_Identity=29.535864978903, Blast_Score=102, Evalue=4e-22,
Organism=Escherichia coli, GI1788588, Length=322, Percent_Identity=99.0683229813665, Blast_Score=660, Evalue=0.0,
Organism=Escherichia coli, GI1788692, Length=315, Percent_Identity=31.7460317460317, Blast_Score=166, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI71999402, Length=236, Percent_Identity=25.8474576271186, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6325441, Length=228, Percent_Identity=25.8771929824561, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24585265, Length=235, Percent_Identity=28.936170212766, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022857
- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: =2.7.8.30 [H]

Molecular weight: Translated: 36296; Mature: 36296

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEIHPVKKVSVVIPVYNEQESLPELIRRTTKACESLGKEYEILLIDDGSSDNSAHMLVD
CCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEE
ASQAEGSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDADLQNPPEEIPRLVAKADEG
CCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCC
YDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYGCMLRAYRRHIVDAMLHCHER
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
STFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLINLMYDLVTCLTTTPLRMLSLLG
CHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAVLFTFIGAQFIGMGLLGEYIGRIYT
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVRARPRYFVQQVIRPSSKENE
HHHCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MFEIHPVKKVSVVIPVYNEQESLPELIRRTTKACESLGKEYEILLIDDGSSDNSAHMLVD
CCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEE
ASQAEGSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDADLQNPPEEIPRLVAKADEG
CCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCC
YDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYGCMLRAYRRHIVDAMLHCHER
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
STFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLINLMYDLVTCLTTTPLRMLSLLG
CHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAVLFTFIGAQFIGMGLLGEYIGRIYT
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVRARPRYFVQQVIRPSSKENE
HHHCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA