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The map label for this gene is glpA

Identifier: 218700713

GI number: 218700713

Start: 2470313

End: 2471941

Strand: Direct

Name: glpA

Synonym: ECIAI39_2384

Alternate gene names: 218700713

Gene position: 2470313-2471941 (Clockwise)

Preceding gene: 218700710

Following gene: 218700714

Centisome position: 48.13

GC content: 57.83

Gene sequence:

>1629_bases
ATGAAAACTCGCGACTCGCAATCAAGTGACGTGATTATCATTGGCGGCGGCGCAACGGGAGCCGGGATTGCCCGCGACTG
TGCCCTGCGCGGGCTGCGCGTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGC
TGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGC
ATTGCACGTCACTGCGTTGAACCGACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTT
TATCCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTA
ACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGACCGCAGCAAATATGCTTGAT
GCCAAAGAACACGGTGCCGTTATCCTTACCGCTCATGAAGTCACGGGGCTGATTCGTGAAGGCGCGACGGTGTGCGGCGT
TCATGTACGTAACCACCTCACCGGCGAAACCCAGGCACTTCATGCGCCTGTCGTGGTTAATGCCGCCGGGATCTGGGGGC
AACATATTGCGGAATATGCCGATCTGCGCATTCGCATGTTCCCGGCGAAAGGGTCGCTGCTGATTATGGATCACCGCATT
AATCAGCATGTGATCAACCGCTGCCGTAAACCTTCTGACGCCGATATTCTGGTGCCTGGCGATACCATTTCGCTAATTGG
CACCACCTCTTTACGTATTGATTACAACGAGATTGACGATAATCGAGTGACGGCAGAAGAAGTTGATATTCTGCTGCGTG
AAGGGGAAAAACTGGCCCCCGTGATGGCGAAAACGCGCATTTTGCGAGCCTACTCTGGCGTACGCCCGCTGGTTGCCAGC
GATGACGATCCGAGCGGACGTAACGTCAGCCGTGGCATCGTGCTGCTCGACCATGCTGAACGCGATGGTCTGGACGGGTT
TATCACCATCACCGGTGGCAAACTGATGACCTACCGCCTGATGGCGGAATGGGCTACTGACGCGGTGTGTCGCAAGCTGG
GCAACACGCGCCCCTGTACCACTGCCGACACGCCGTTGCCTGGCTCACAGGAACCGGCAGAAGTCACGCTGCGCAAAGTG
ATCTCCCTGCCCGCTCCGCTGCGCGGTTCCGCAGTTTACCGGCACGGCGATCGCACACCAGCCTGGCTGAGCGAAGGCCG
CCTGCACCGCAGCCTGGTATGTGAGTGCGAAGCGGTAACTGCGGGTGAAGTGCAGTACGCAGTAGAAAATTTAAACGTTA
ACAGCCTGCTGGATTTACGCCGTCGTACCCGTGTAGGGATGGGTACCTGCCAGGGCGAACTCTGCGCCTGCCGGGCTGCC
GGACTGCTGCAACGTTTTAACGTCACGACGTCCGCGCAATCTATCGAGCAACTTTCCACCTTCCTTAACGAACGCTGGAA
AGGCGTGCAACCCATAGCATGGGGAGACGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGACTGTGTGGTC
TGGAGAAGGAGCAGAAAGATGCGCTTTGA

Upstream 100 bases:

>100_bases
CACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAA
CGAACTTCAGAGGGATAACA

Downstream 100 bases:

>100_bases
TACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGACCTGCGCTGTGCCATTG
TCACTCGTGGTCAAAGCGCG

Product: sn-glycerol-3-phosphate dehydrogenase subunit A

Products: NA

Alternate protein names: G-3-P dehydrogenase [H]

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIATGATGRNHGLLHSGARYAVTDAESARECISENQILKR
IARHCVEPTNGLFITLPEDDLSFQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
AKEHGAVILTAHEVTGLIREGATVCGVHVRNHLTGETQALHAPVVVNAAGIWGQHIAEYADLRIRMFPAKGSLLIMDHRI
NQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYNEIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVAS
DDDPSGRNVSRGIVLLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADTPLPGSQEPAEVTLRKV
ISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVENLNVNSLLDLRRRTRVGMGTCQGELCACRAA
GLLQRFNVTTSAQSIEQLSTFLNERWKGVQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL

Sequences:

>Translated_542_residues
MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIATGATGRNHGLLHSGARYAVTDAESARECISENQILKR
IARHCVEPTNGLFITLPEDDLSFQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
AKEHGAVILTAHEVTGLIREGATVCGVHVRNHLTGETQALHAPVVVNAAGIWGQHIAEYADLRIRMFPAKGSLLIMDHRI
NQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYNEIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVAS
DDDPSGRNVSRGIVLLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADTPLPGSQEPAEVTLRKV
ISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVENLNVNSLLDLRRRTRVGMGTCQGELCACRAA
GLLQRFNVTTSAQSIEQLSTFLNERWKGVQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL
>Mature_542_residues
MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIATGATGRNHGLLHSGARYAVTDAESARECISENQILKR
IARHCVEPTNGLFITLPEDDLSFQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
AKEHGAVILTAHEVTGLIREGATVCGVHVRNHLTGETQALHAPVVVNAAGIWGQHIAEYADLRIRMFPAKGSLLIMDHRI
NQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYNEIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVAS
DDDPSGRNVSRGIVLLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADTPLPGSQEPAEVTLRKV
ISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVENLNVNSLLDLRRRTRVGMGTCQGELCACRAA
GLLQRFNVTTSAQSIEQLSTFLNERWKGVQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL

Specific function: Conversion of glycerol 3-phosphate to dihydroxyacetone. Uses fumarate or nitrate as electron acceptor [H]

COG id: COG0578

COG function: function code C; Glycerol-3-phosphate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=Loosely bound to the cytoplasmic membrane often occurring in vesicles associated with fumarate reductase (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI285002233, Length=398, Percent_Identity=26.6331658291457, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI285002231, Length=398, Percent_Identity=26.6331658291457, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1788574, Length=542, Percent_Identity=99.4464944649446, Blast_Score=1098, Evalue=0.0,
Organism=Escherichia coli, GI2367226, Length=380, Percent_Identity=25.5263157894737, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI115534726, Length=417, Percent_Identity=26.8585131894484, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17509253, Length=414, Percent_Identity=29.4685990338164, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI161077125, Length=398, Percent_Identity=29.1457286432161, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24653942, Length=398, Percent_Identity=29.1457286432161, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24653944, Length=398, Percent_Identity=29.1457286432161, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI20130025, Length=398, Percent_Identity=29.1457286432161, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24586295, Length=394, Percent_Identity=25.1269035532995, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR006076
- InterPro:   IPR017752
- InterPro:   IPR000447 [H]

Pfam domain/function: PF01266 DAO; PF04324 Fer2_BFD [H]

EC number: =1.1.5.3 [H]

Molecular weight: Translated: 58954; Mature: 58954

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS00977 FAD_G3PDH_1 ; PS00978 FAD_G3PDH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIATGATGRNHGLLHSGARY
CCCCCCCCCCEEEEECCCCCCCHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCEECCCEE
AVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLSFQATFIRACEEAGISAEA
EEECHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCC
IDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLDAKEHGAVILTAHEVTGLIRE
CCCHHHEEECCCCCCEEEEEEECCCCCCCCEEEHHHHHHCHHHCCEEEEEHHHHHHHHHC
GATVCGVHVRNHLTGETQALHAPVVVNAAGIWGQHIAEYADLRIRMFPAKGSLLIMDHRI
CCEEEEEEHHHCCCCCCCEEECCEEEEECCHHHHHHHHHHCEEEEEEECCCCEEEEECCC
NQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYNEIDDNRVTAEEVDILLREGEKLAP
CHHHHHHCCCCCCCEEEECCCEEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHCCCHHHH
VMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIVLLDHAERDGLDGFITITGGKLMTYRL
HHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCEEEHHH
MAEWATDAVCRKLGNTRPCTTADTPLPGSQEPAEVTLRKVISLPAPLRGSAVYRHGDRTP
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCC
AWLSEGRLHRSLVCECEAVTAGEVQYAVENLNVNSLLDLRRRTRVGMGTCQGELCACRAA
HHHHCCCCCHHHEEEECCCCCCHHEEHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
GLLQRFNVTTSAQSIEQLSTFLNERWKGVQPIAWGDALRESEFTRWVYQGLCGLEKEQKD
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHC
AL
CC
>Mature Secondary Structure
MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIATGATGRNHGLLHSGARY
CCCCCCCCCCEEEEECCCCCCCHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCEECCCEE
AVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLSFQATFIRACEEAGISAEA
EEECHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCC
IDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLDAKEHGAVILTAHEVTGLIRE
CCCHHHEEECCCCCCEEEEEEECCCCCCCCEEEHHHHHHCHHHCCEEEEEHHHHHHHHHC
GATVCGVHVRNHLTGETQALHAPVVVNAAGIWGQHIAEYADLRIRMFPAKGSLLIMDHRI
CCEEEEEEHHHCCCCCCCEEECCEEEEECCHHHHHHHHHHCEEEEEEECCCCEEEEECCC
NQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYNEIDDNRVTAEEVDILLREGEKLAP
CHHHHHHCCCCCCCEEEECCCEEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHCCCHHHH
VMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIVLLDHAERDGLDGFITITGGKLMTYRL
HHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCEEEEECCCCCCCCEEEEEECCCEEEHHH
MAEWATDAVCRKLGNTRPCTTADTPLPGSQEPAEVTLRKVISLPAPLRGSAVYRHGDRTP
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCC
AWLSEGRLHRSLVCECEAVTAGEVQYAVENLNVNSLLDLRRRTRVGMGTCQGELCACRAA
HHHHCCCCCHHHEEEECCCCCCHHEEHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
GLLQRFNVTTSAQSIEQLSTFLNERWKGVQPIAWGDALRESEFTRWVYQGLCGLEKEQKD
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHC
AL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]