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The map label for this gene is cspD

Identifier: 218700605

GI number: 218700605

Start: 2334829

End: 2335053

Strand: Direct

Name: cspD

Synonym: ECIAI39_2272

Alternate gene names: 218700605

Gene position: 2334829-2335053 (Clockwise)

Preceding gene: 218700601

Following gene: 218700608

Centisome position: 45.49

GC content: 48.44

Gene sequence:

>225_bases
ATGGAAAAGGGTACTGTTAAGTGGTTCAACAATGCCAAAGGGTTTGGTTTCATCTGCCCTGAAGGCGGCGGCGAAGATAT
TTTCGCTCATTATTCCACCATTCAGATGGATGGTTACAGAACGCTAAAAGCTGGACAATCCGTTCAGTTTGATGTCCACC
AGGGGCCAAAAGGCAATCACGCCAGTGTTATTGTGCCCGTCGAAGTAGAAGCGGCAGTCGCATAG

Upstream 100 bases:

>100_bases
TAAATTGTATTTCTAGAGTTGGCGAGGTTTTGAACAGCCCCCTCTCTGACCCCGGTTTATTCCATCTTACTTGTATAAGA
TTTGCGAAGGATGTCGAAGC

Downstream 100 bases:

>100_bases
CTCTTCTGTCTCATTGTGTACATCCTAAAGGCAAAATGCCAGCCCGATCGGCTGGCATTTTTATCTCAAAAATTACTCTC
GTGCCAGAGCATCTACTGGA

Product: stationary phase/starvation inducible regulatory protein CspD

Products: NA

Alternate protein names: CSP-D

Number of amino acids: Translated: 74; Mature: 74

Protein sequence:

>74_residues
MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKAGQSVQFDVHQGPKGNHASVIVPVEVEAAVA

Sequences:

>Translated_74_residues
MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKAGQSVQFDVHQGPKGNHASVIVPVEVEAAVA
>Mature_74_residues
MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKAGQSVQFDVHQGPKGNHASVIVPVEVEAAVA

Specific function: Inhibits DNA replication at both initiation and elongation steps, most probably by binding to the opened, single- stranded regions at replication forks. Plays a regulatory role in chromosomal replication in nutrient-depleted cells

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain

Homologues:

Organism=Escherichia coli, GI1787107, Length=74, Percent_Identity=100, Blast_Score=155, Evalue=6e-40,
Organism=Escherichia coli, GI1786841, Length=65, Percent_Identity=50.7692307692308, Blast_Score=80, Evalue=3e-17,
Organism=Escherichia coli, GI1788126, Length=65, Percent_Identity=49.2307692307692, Blast_Score=74, Evalue=1e-15,
Organism=Escherichia coli, GI1789979, Length=64, Percent_Identity=50, Blast_Score=73, Evalue=4e-15,
Organism=Escherichia coli, GI1787839, Length=62, Percent_Identity=53.2258064516129, Blast_Score=73, Evalue=4e-15,
Organism=Escherichia coli, GI2367114, Length=64, Percent_Identity=46.875, Blast_Score=72, Evalue=8e-15,
Organism=Escherichia coli, GI1787834, Length=62, Percent_Identity=50, Blast_Score=71, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24658520, Length=61, Percent_Identity=42.6229508196721, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CSPD_ECO57 (P0A970)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B85600
- PIR:   F90749
- RefSeq:   NP_286652.1
- RefSeq:   NP_308993.1
- ProteinModelPortal:   P0A970
- SMR:   P0A970
- MINT:   MINT-1219773
- EnsemblBacteria:   EBESCT00000024033
- EnsemblBacteria:   EBESCT00000055757
- GeneID:   917710
- GeneID:   958429
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1117
- KEGG:   ecs:ECs0966
- GeneTree:   EBGT00070000032049
- HOGENOM:   HBG717773
- OMA:   QVVRFDV
- ProtClustDB:   PRK09937
- BioCyc:   ECOL83334:ECS0966-MONOMER
- GO:   GO:0005737
- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012751
- InterPro:   IPR012340
- InterPro:   IPR016027
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF002599
- PRINTS:   PR00050
- SMART:   SM00357
- TIGRFAMs:   TIGR02381

Pfam domain/function: PF00313 CSD; SSF50249 Nucleic_acid_OB

EC number: NA

Molecular weight: Translated: 7969; Mature: 7969

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKAGQSVQFDVHQGPKGNH
CCCCCEEEECCCCCEEEECCCCCCCCEEEEEEEEEECCEEEECCCCEEEEEECCCCCCCC
ASVIVPVEVEAAVA
CEEEEEEEEEEECC
>Mature Secondary Structure
MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKAGQSVQFDVHQGPKGNH
CCCCCEEEECCCCCEEEECCCCCCCCEEEEEEEEEECCEEEECCCCEEEEEECCCCCCCC
ASVIVPVEVEAAVA
CEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796