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The map label for this gene is aat

Identifier: 218700599

GI number: 218700599

Start: 2329745

End: 2330449

Strand: Direct

Name: aat

Synonym: ECIAI39_2265

Alternate gene names: 218700599

Gene position: 2329745-2330449 (Clockwise)

Preceding gene: 218700598

Following gene: 218700600

Centisome position: 45.4

GC content: 54.04

Gene sequence:

>705_bases
ATGCGCCTGGTTCAGCTTTCTCGCCATTCAATAGCCTTCCCTTCCCCGGAAGGCGCATTACGTGAGCCTAACGGCCTGCT
GGCACTGGGGGGCGATCTTAGCCCTGCGCGCCTGTTAATGGCTTACCAGCGTGGTATTTTTCCGTGGTTTTCTCCCGGCG
ACCCCATCCTCTGGTGGTCGCCCGATCCCCGCGCGGTGCTATGGCCAGAATCACTGCATATCAGCCGTAGTATGAAGCGA
TTTCATAAACGCTCGCCCTATCGTGTCACGATGAATTACGCCTTTGGTCAGGTCATTGAAGGCTGTGCCAGCGATCGCGA
AGAAGGAACCTGGATCACGCGTGGCGTGGTCGAAGCCTACCATCGTCTTCACGAACTCGGTCATGCCCACTCCATCGAAG
TCTGGTGTGAAGATGAGCTTGTTGGCGGTATGTACGGTGTGGCCCAGGGAACGCTATTTTGTGGCGAGTCCATGTTCAGC
CGGATGGAAAACGCGTCCAAAACGGCGCTACTGGTCTTCTGTGAGGAATTTATTGGTCATGGCGGTAAACTTATCGACTG
CCAGGTCCTTAACGATCACACAGCTTCGCTTGGTGCCTGCGAAATTCCCCGCCGGGATTATCTAAATTATCTCAATCAAA
TGCGCCTCGGACGATTGCCGAATAATTTCTGGGTACCACGGTGCTTGTTTTCACCACAAGAATGA

Upstream 100 bases:

>100_bases
CTCACGCAGAACTGCTTGCCAGACAGGGGCGTTATTACCAGTTTAAGCAGGGTTTGTAAGCTATTATTGAACGATCCGAC
TTGCGTGGAGTTTTGCGGTC

Downstream 100 bases:

>100_bases
ATGTTTTCGGCACATTTCTCCCCAGAGTGTTATAATTGCGGTCGCAGAGTTGGTTACGCTCATTACCCCGCTGCCGATAA
GGAATTTTTCGCGTCAGGTA

Product: leucyl/phenylalanyl-tRNA--protein transferase

Products: NA

Alternate protein names: L/F-transferase; Leucyltransferase; Phenyalanyltransferase [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQRGIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKR
FHKRSPYRVTMNYAFGQVIEGCASDREEGTWITRGVVEAYHRLHELGHAHSIEVWCEDELVGGMYGVAQGTLFCGESMFS
RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGRLPNNFWVPRCLFSPQE

Sequences:

>Translated_234_residues
MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQRGIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKR
FHKRSPYRVTMNYAFGQVIEGCASDREEGTWITRGVVEAYHRLHELGHAHSIEVWCEDELVGGMYGVAQGTLFCGESMFS
RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGRLPNNFWVPRCLFSPQE
>Mature_234_residues
MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQRGIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKR
FHKRSPYRVTMNYAFGQVIEGCASDREEGTWITRGVVEAYHRLHELGHAHSIEVWCEDELVGGMYGVAQGTLFCGESMFS
RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGRLPNNFWVPRCLFSPQE

Specific function: Functions in the N-end rule pathway of protein degradation where it conjugates Leu, Phe and, less efficiently, Met from aminoacyl-tRNAs to the N-termini of proteins containing an N-terminal arginine or lysine [H]

COG id: COG2360

COG function: function code O; Leu/Phe-tRNA-protein transferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L/F-transferase family [H]

Homologues:

Organism=Escherichia coli, GI1787111, Length=234, Percent_Identity=99.5726495726496, Blast_Score=483, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016181
- InterPro:   IPR004616 [H]

Pfam domain/function: PF03588 Leu_Phe_trans [H]

EC number: =2.3.2.6 [H]

Molecular weight: Translated: 26566; Mature: 26566

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQRGIFPWFSPGDPILWWS
CCEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEEC
PDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGCASDREEGTWITRGVVEAY
CCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHH
HRLHELGHAHSIEVWCEDELVGGMYGVAQGTLFCGESMFSRMENASKTALLVFCEEFIGH
HHHHHHCCCCEEEEEECCCHHCCHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
GGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGRLPNNFWVPRCLFSPQE
CCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQRGIFPWFSPGDPILWWS
CCEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCEEEEC
PDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGCASDREEGTWITRGVVEAY
CCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHH
HRLHELGHAHSIEVWCEDELVGGMYGVAQGTLFCGESMFSRMENASKTALLVFCEEFIGH
HHHHHHCCCCEEEEEECCCHHCCHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
GGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGRLPNNFWVPRCLFSPQE
CCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA