Click here to switch to the map view.

The map label for this gene is yccW

Identifier: 218700512

GI number: 218700512

Start: 2224626

End: 2225729

Strand: Direct

Name: yccW

Synonym: ECIAI39_2178

Alternate gene names: 218700512

Gene position: 2224626-2225729 (Clockwise)

Preceding gene: 218700510

Following gene: 218700513

Centisome position: 43.35

GC content: 51.63

Gene sequence:

>1104_bases
ATGGAAGGTAAAGCCAGCCTCGGTGAAACCATCGATATTGTTGATCATCAGGGAAAATGGTTAGCACGCGGCGCTTATTC
GCCAACTTCGCAAATCCGGGCGCGCGTCTGGACGTTTGACCCGTCTGAGTCTATCGACATTGCTTTTTTTTCCCGCCGTT
TGCAACAAGCACAAAAATGGCGTGACTGGCTGGCGCAAAAAGATGGCCTCGACAGTTATCGTTTAATCGCCGGAGAATCT
GATGGCCTGCCGGGTATTACTATCGATCGTTTCGGTAATTTTCTGGTGCTGCAACTGCTGAGTGCTGGCGCAGAATATCA
GCGCGCGGCATTAATTAGTGCCCTGCAAACGCTGTACCCGGAATGTGCGATTTACGATCGCAGCGATGTTGCGGTACGTA
AAAAAGAAGGGATGGAGCTGACCCAGGGCCCCGTCACCGGCGAGTTGCCGCCTGCCCTGCTGCCGATTGAAGAACACGGC
ATGAAGCTGCTGGTGGACATACAGCACGGACACAAAACGGGCTACTACCTGGATCAGCGTGATAGCCGCCTGGCTACCCG
CCGCTACGTTGAAAATAAACGCGTACTGAACTGTTTCTCCTATACCGGTGGTTTCGCCGTATCGGCACTGATGGGCGGTT
GCAGCCAGGTTGTCAGCGTTGATACCTCCCAGGAAGCCCTGGATATTGCACGACAGAACGTTGAGCTGAACAAACTGGAT
CTGAGCAAGGCTGAGTTTGTCCGTGATGATGTCTTTAAATTGCTGCGTACCTATCGCGATCGCGGTGAAAAATTTGACGT
TATCGTGATGGACCCGCCGAAGTTTGTTGAGAATAAAAGCCAGTTGATGGGCGCGTGTCGTGGCTATAAAGATATCAACA
TGCTGGCGATTCAGTTGCTGAATGAAGGCGGTATTCTCCTGACTTTCTCCTGTTCTGGCCTGATGACCAGCGATTTATTT
CAGAAAATCATCGCGGATGCCGCAATTGATGCCGGTCGTGATGTACAATTTATAGAGCAGTTCCGTCAGGCAGCCGATCA
TCCGGTGATCGCTACCTATCCGGAAGGGCTATATCTGAAAGGGTTTGCCTGTCGCGTCATGTAA

Upstream 100 bases:

>100_bases
AGAAAAGTAAATTATGAGTGTACGTTTAGTGTTAGCCAAAGGGCGCGAAAAATCATTACTTCGTCGCCATCCGTGGATCT
TTTCCGGGGCCGTTGCCCGC

Downstream 100 bases:

>100_bases
CTTGAAATGTGGAATTTTACCCTTATATCAAAGGTAAAGATTTCCCGGGAGGTGACTATGATTGCCAGCAAATTCGGTAT
CGGCCAGCAGGTCCGCCATT

Product: putative AdoMet-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MEGKASLGETIDIVDHQGKWLARGAYSPTSQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSYRLIAGES
DGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVAVRKKEGMELTQGPVTGELPPALLPIEEHG
MKLLVDIQHGHKTGYYLDQRDSRLATRRYVENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLD
LSKAEFVRDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGILLTFSCSGLMTSDLF
QKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFACRVM

Sequences:

>Translated_367_residues
MEGKASLGETIDIVDHQGKWLARGAYSPTSQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSYRLIAGES
DGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVAVRKKEGMELTQGPVTGELPPALLPIEEHG
MKLLVDIQHGHKTGYYLDQRDSRLATRRYVENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLD
LSKAEFVRDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGILLTFSCSGLMTSDLF
QKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFACRVM
>Mature_367_residues
MEGKASLGETIDIVDHQGKWLARGAYSPTSQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSYRLIAGES
DGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVAVRKKEGMELTQGPVTGELPPALLPIEEHG
MKLLVDIQHGHKTGYYLDQRDSRLATRRYVENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLD
LSKAEFVRDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGILLTFSCSGLMTSDLF
QKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFACRVM

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=367, Percent_Identity=99.4550408719346, Blast_Score=755, Evalue=0.0,
Organism=Escherichia coli, GI1787181, Length=241, Percent_Identity=29.045643153527, Blast_Score=86, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 41068; Mature: 41068

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGKASLGETIDIVDHQGKWLARGAYSPTSQIRARVWTFDPSESIDIAFFSRRLQQAQKW
CCCCCCCCCEEEEECCCCCEEECCCCCCHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHH
RDWLAQKDGLDSYRLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYP
HHHHHHCCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
ECAIYDRSDVAVRKKEGMELTQGPVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQR
CCEEECCCCCEEEHHCCCEECCCCCCCCCCCCCCCHHHCCCEEEEEEECCCCCCCEECCC
DSRLATRRYVENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLD
CHHHHHHHHHHCCHHEEHHHCCCHHHHHHHHHCHHHHEECCCCHHHHHHHHHCCCCCEEC
LSKAEFVRDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLL
CHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHCCHHHHHHHHCCCCHHHHHHHHHH
NEGGILLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLK
CCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEECCCCEEEC
GFACRVM
CEEEECC
>Mature Secondary Structure
MEGKASLGETIDIVDHQGKWLARGAYSPTSQIRARVWTFDPSESIDIAFFSRRLQQAQKW
CCCCCCCCCEEEEECCCCCEEECCCCCCHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHH
RDWLAQKDGLDSYRLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYP
HHHHHHCCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
ECAIYDRSDVAVRKKEGMELTQGPVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQR
CCEEECCCCCEEEHHCCCEECCCCCCCCCCCCCCCHHHCCCEEEEEEECCCCCCCEECCC
DSRLATRRYVENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLD
CHHHHHHHHHHCCHHEEHHHCCCHHHHHHHHHCHHHHEECCCCHHHHHHHHHCCCCCEEC
LSKAEFVRDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLL
CHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHCCHHHHHHHHCCCCHHHHHHHHHH
NEGGILLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLK
CCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEECCCCEEEC
GFACRVM
CEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA