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The map label for this gene is tusE

Identifier: 218700510

GI number: 218700510

Start: 2223840

End: 2224169

Strand: Direct

Name: tusE

Synonym: ECIAI39_2176

Alternate gene names: 218700510

Gene position: 2223840-2224169 (Clockwise)

Preceding gene: 218700509

Following gene: 218700512

Centisome position: 43.33

GC content: 48.18

Gene sequence:

>330_bases
ATGCTGATCTTCGAAGGTAAAGAGATAGAAACGGATACCGAAGGCTATCTCAAAGAAAGCAGCCAGTGGAGTGAGCCACT
GGCGGTGGTGATTGCAGAGAACGAAGGGATTGCGCTGTCGCCAGAACACTGGGAAGTGGTGCGTTTTGTGCGTGATTTCT
ATCTGGAATTCAATACTTCTCCGGCGATTCGTATGCTGGTAAAAGCGATGGCAAATAAATTTGGCGAAGAGAAAGGCAAT
AGCCGCTATCTGTACCGACTGTTCCCGAAAGGTCCGGCAAAGCAAGCCACCAAAATTGCTGGCCTGCCTAAACCGGTAAA
ATGCATTTAA

Upstream 100 bases:

>100_bases
CGCGATTAATCTCACCCGCTAACACTTTCATAGCCTCGCTTTATGCGGGGCTTTGTTTTTTGTTACACTGCCGCCAGTCT
GATTAAGGTAGTAAAGAATT

Downstream 100 bases:

>100_bases
CGGATACGAAAATCCGTTAACTCCCCGGAGGGATGATGCGGCTCGCTAAGCACCCGTTCCACACGCGCTGAACGCGGGCC
GCCACTTTTTAACCACTGCA

Product: sulfur transfer protein TusE

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein E [H]

Number of amino acids: Translated: 109; Mature: 109

Protein sequence:

>109_residues
MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGIALSPEHWEVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGN
SRYLYRLFPKGPAKQATKIAGLPKPVKCI

Sequences:

>Translated_109_residues
MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGIALSPEHWEVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGN
SRYLYRLFPKGPAKQATKIAGLPKPVKCI
>Mature_109_residues
MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGIALSPEHWEVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGN
SRYLYRLFPKGPAKQATKIAGLPKPVKCI

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions. Could accept sulfur from tusD [H]

COG id: COG2920

COG function: function code P; Dissimilatory sulfite reductase (desulfoviridin), gamma subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dsrC/tusE family [H]

Homologues:

Organism=Escherichia coli, GI87081811, Length=109, Percent_Identity=99.0825688073395, Blast_Score=224, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007453 [H]

Pfam domain/function: PF04358 DsrC [H]

EC number: NA

Molecular weight: Translated: 12394; Mature: 12394

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGIALSPEHWEVVRFVRDFYLEFNTS
CEEECCCCCCCCCCHHHHCCCCCCCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHCCCC
PAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGLPKPVKCI
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGIALSPEHWEVVRFVRDFYLEFNTS
CEEECCCCCCCCCCHHHHCCCCCCCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHCCCC
PAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGLPKPVKCI
HHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]