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The map label for this gene is rutB

Identifier: 218700478

GI number: 218700478

Start: 2190867

End: 2191559

Strand: Direct

Name: rutB

Synonym: ECIAI39_2144

Alternate gene names: 218700478

Gene position: 2190867-2191559 (Clockwise)

Preceding gene: 218700477

Following gene: 218700479

Centisome position: 42.69

GC content: 54.69

Gene sequence:

>693_bases
ATGACAACCTTAACCGCACGACCGGAAGCCATTACCTTCGATCCGCAGCAAACTGCGCTGATCGTGGTGGATATGCAAAA
TGCCTATGCCACGCCAGGCGGCTACTTAGATCTCGCAGGGTTTGATGTCTCAACCACTCGCCCGGTCATTGCCAACATTC
AAACCGCCGTGACCGCAGCGCGAGCGGCAGGGATGTTGATTATCTGGTTTCAAAATGGCTGGGATGCACAGTATGTCGAG
GCTGGCGGCCCCGGCTCACCAAATTTTCATAAATCGAACGCCCTGAAAACCATGCGTAAGCAGCCGCAGCTACAGGGGAA
ATTGCTGGCGAAAGGCTCCTGGGATTATCAACTGGTGGATGAACTGGTGCCGCAGCCTGGCGATATTGTGCTGCCGAAGC
CGCGCTACAGCAGTTTCTTCAATACGCCGCTGGACAGCATTTTGCGCAGCCGTGGAATACGCCATCTGGTTTTCACCGGC
ATCGCTACCAACGTCTGCGTCGAATCGACGCTACGCGATGGCTTTTTTCTGGAGTATTTCGGCGTGGTGCTGGAAGACGC
AACACACCAGGCGGGGCCGGAATTTGCACAGAAAGCCGCGTTGTTCAATATCGAAACCTTTTTTGGCTGGGTAAGCGACG
TCGAAACGTTCTGCGACGCGCTTTCTCCCACGTCCTTTGCTCGTATCGCTTAA

Upstream 100 bases:

>100_bases
ACCAACGTCCGTCAGATGGCCGATCCCACTTCGGCGAGCGCATTCAACCACTGATGCAGTGTCGCGCCCATCTCCCTGCG
CTGACTCAGGAGGTGGCATG

Downstream 100 bases:

>100_bases
GGAGTTTAACGATGCCAAAATCCGTAATTATTCCCGCTGGCAGCAGCGCACCGCTGGCTCCCTTCGTTCCCGGCACGCTG
GCTGATGGCGTGGTGTATGT

Product: alternative pyrimidine degradation pathway protein

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MTTLTARPEAITFDPQQTALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLIIWFQNGWDAQYVE
AGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSSFFNTPLDSILRSRGIRHLVFTG
IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSPTSFARIA

Sequences:

>Translated_230_residues
MTTLTARPEAITFDPQQTALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLIIWFQNGWDAQYVE
AGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSSFFNTPLDSILRSRGIRHLVFTG
IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSPTSFARIA
>Mature_229_residues
TTLTARPEAITFDPQQTALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLIIWFQNGWDAQYVEA
GGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSSFFNTPLDSILRSRGIRHLVFTGI
ATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSPTSFARIA

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=230, Percent_Identity=97.8260869565217, Blast_Score=467, Evalue=1e-133,
Organism=Escherichia coli, GI87081992, Length=211, Percent_Identity=29.3838862559242, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 25215; Mature: 25084

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLTARPEAITFDPQQTALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAA
CCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHH
RAAGMLIIWFQNGWDAQYVEAGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVD
HHCCEEEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHH
ELVPQPGDIVLPKPRYSSFFNTPLDSILRSRGIRHLVFTGIATNVCVESTLRDGFFLEYF
HHCCCCCCEEECCCCHHHHHCCCHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCHHHHHH
GVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSPTSFARIA
HHHHHHCCCCCCHHHHHHHHHEEHHHHHHHHHHHHHHHHHCCCCCHHHCC
>Mature Secondary Structure 
TTLTARPEAITFDPQQTALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAA
CCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHH
RAAGMLIIWFQNGWDAQYVEAGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVD
HHCCEEEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHH
ELVPQPGDIVLPKPRYSSFFNTPLDSILRSRGIRHLVFTGIATNVCVESTLRDGFFLEYF
HHCCCCCCEEECCCCHHHHHCCCHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCHHHHHH
GVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSPTSFARIA
HHHHHHCCCCCCHHHHHHHHHEEHHHHHHHHHHHHHHHHHCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA