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The map label for this gene is ymfB

Identifier: 218700343

GI number: 218700343

Start: 2054190

End: 2054651

Strand: Direct

Name: ymfB

Synonym: ECIAI39_2003

Alternate gene names: 218700343

Gene position: 2054190-2054651 (Clockwise)

Preceding gene: 218700342

Following gene: 218700344

Centisome position: 40.03

GC content: 50.43

Gene sequence:

>462_bases
ATGTTTAAACCGCACGTTACCGTTGCTTGCGTGGTGCACGCAGAAGGCAAATTTTTAGTCGTTGAAGAGACGATTAATGG
TAAAGCGTTATGGAACCAACCTGCCGGGCATCTGGAAGCCGATGAAACCTTAGTGGAAGCCGCCGCCCGTGAGCTGTGGG
AAGAAACCGGCATCAGCGCGCAGCCGCAACACTTTATTCGTATGCATCAGTGGATTGCACCAGATAAAACGCCGTTTTTG
CGTTTCCTCTTTGCCATTGAGCTTGAGCAAATATGCCCGACCCAGCCTCATGACAGCGATATCGACTGCTGCCGTTGGGT
TAGCGCCGAAGAAATTTTACAGGCGTCAAATCTGCGTTCGCCGCTGGTGGCGGAAAGTATTCGTTGTTATCAAAGCGGGC
AACGCTATCCGCTGGAGATGATTGGCGATTTTAACTGGCCTTTTACAAAGGGTGTCATCTAA

Upstream 100 bases:

>100_bases
TTTCCCCACGCTGCGACTGATTCGCTATGCGATGGGTGATTACTCTTTGGATAATCTTGCCAACGGCGAATGGCGAGAAG
CGACAGATTAAGGACGCAAA

Downstream 100 bases:

>100_bases
AAGGCTGCGTGATAGAATACGCCGCCTTGAAGTTCAATGTCGTGAGTGATCCAATGTCTGAAACCGCAAAAAAAGTAATC
GTCGGCATGTCCGGCGGTGT

Product: bifunctional thiamin pyrimidine pyrophosphate hydrolase and thiamin pyrophosphate hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 153; Mature: 153

Protein sequence:

>153_residues
MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETLVEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFL
RFLFAIELEQICPTQPHDSDIDCCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI

Sequences:

>Translated_153_residues
MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETLVEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFL
RFLFAIELEQICPTQPHDSDIDCCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI
>Mature_153_residues
MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETLVEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFL
RFLFAIELEQICPTQPHDSDIDCCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain

Homologues:

Organism=Escherichia coli, GI1787379, Length=153, Percent_Identity=100, Blast_Score=320, Evalue=3e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUDJ_ECO24 (A7ZKS4)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001462403.1
- ProteinModelPortal:   A7ZKS4
- SMR:   A7ZKS4
- STRING:   A7ZKS4
- EnsemblBacteria:   EBESCT00000019020
- GeneID:   5590863
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_1297
- eggNOG:   COG0494
- GeneTree:   EBGT00050000009958
- HOGENOM:   HBG290030
- OMA:   FLLVEEE
- ProtClustDB:   CLSK879970
- BioCyc:   ECOL331111:ECE24377A_1297-MONOMER
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797
- Gene3D:   G3DSA:3.90.79.10
- PRINTS:   PR00502

Pfam domain/function: PF00293 NUDIX; SSF55811 NUDIX_hydrolase

EC number: 3.6.-.- [C]

Molecular weight: Translated: 17433; Mature: 17433

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS51462 NUDIX; PS00893 NUDIX_BOX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETLVEAAARELWEETGISA
CCCCCEEEEEEEECCCCEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCC
QPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDIDCCRWVSAEEILQASNLRS
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHCCCC
PLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI
CHHHHHHHHHHCCCCCCHHHCCCCCCCHHCCCC
>Mature Secondary Structure
MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETLVEAAARELWEETGISA
CCCCCEEEEEEEECCCCEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCC
QPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDIDCCRWVSAEEILQASNLRS
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHCCCC
PLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI
CHHHHHHHHHHCCCCCCHHHCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA