Click here to switch to the map view.

The map label for this gene is sitA

Identifier: 218700265

GI number: 218700265

Start: 1994537

End: 1995451

Strand: Direct

Name: sitA

Synonym: ECIAI39_1919

Alternate gene names: 218700265

Gene position: 1994537-1995451 (Clockwise)

Preceding gene: 218700263

Following gene: 218700266

Centisome position: 38.86

GC content: 48.42

Gene sequence:

>915_bases
ATGCACTCGATAAAAAAAGTAACCATGCTCTTGGGGGGCCTCGCACTCACCTGCTCGATCGCATTTCAGGCAAGTGCAAC
TGAAAAATTCAAGGTCATTACAACATTTACCATCATCGCGGATATGGCCAAAAACGTGGCGGGAGATGCTGCAGAAGTCT
CATCCATAACCAAGCCTGGTGCAGAAATTCATGAGTATCAGCCTACCCCTGGCGATATTAAACGTGCGCAGGGGGCACAA
CTGATTCTCGCCAATGGTATGAATCTGGAATTGTGGTTCCAACGCTTTTACCAGCATCTCAATGGGGTTCCAGAAGTAAT
TGTCTCTTCGGGTGTGACGCCTGTAGGGATCACCGAAGGACCCTATGAGGGCAAACCTAACCCCCATGCATGGATGTCGC
CAGATAATGCTCTGATTTACGTCGATAATATTCGTGATGCGTTGATAAAATACGATCCGGCAAATGCACAAACCTACCAA
CGCAATGCCGATACTTATAAAGCCAAGATTACCCAAACCCTTGCCCCCCTGCGTAAGCAGATTACGGAACTCCCTGAGAA
TCAGCGATGGATGGTCACCAGTGAAGGGGCTTTTTCTTATCTCGCACGCGATTTGGGGCTAAAAGAGCTTTATCTGTGGC
CGATTAATGCCGATCAACAAGGAACACCGCAGCAGGTACGTAAGGTTGTTGATATAGTTAAGAAAAATCATATCCCGGCA
GTCTTTAGCGAGAGTACGATTTCCGATAAACCAGCGCGTCAGGTTGCGCGTGAAACCGGCGCACACTACGGCGGTGTGCT
CTATGTCGATTCCCTGAGCACAGAAAACGGCCCGGTACCGACGTATATCGACCTTCTTAAGGTTACTACCAGTACGCTGG
TACAGGGAATTAAAGCTGGGAAAAGGGAGAAATAA

Upstream 100 bases:

>100_bases
AAACCGCTAATGATAATCATTTTCATTTTGTATAGGTTGTGCTATAAAACATAGCCGTTGCTATGTTTGTTCTAATTTTG
CTCACTATAGGTACTAAATT

Downstream 100 bases:

>100_bases
TGATGCAATCTGCAGGCATTGTCGTTAATGATGTTACTGTCACCTGGCGTAACGGGCACACAGCACTGCGCGATGCATCC
TTCACAGTACCAAGTGGATC

Product: iron transport protein, periplasmic-binding protein

Products: Zn (II) [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MHSIKKVTMLLGGLALTCSIAFQASATEKFKVITTFTIIADMAKNVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQ
LILANGMNLELWFQRFYQHLNGVPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQVRKVVDIVKKNHIPA
VFSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPVPTYIDLLKVTTSTLVQGIKAGKREK

Sequences:

>Translated_304_residues
MHSIKKVTMLLGGLALTCSIAFQASATEKFKVITTFTIIADMAKNVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQ
LILANGMNLELWFQRFYQHLNGVPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQVRKVVDIVKKNHIPA
VFSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPVPTYIDLLKVTTSTLVQGIKAGKREK
>Mature_304_residues
MHSIKKVTMLLGGLALTCSIAFQASATEKFKVITTFTIIADMAKNVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQ
LILANGMNLELWFQRFYQHLNGVPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQVRKVVDIVKKNHIPA
VFSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPVPTYIDLLKVTTSTLVQGIKAGKREK

Specific function: Part of an ATP-driven transport system HI_0359/HI_0360/HI_0361/HI_0362 for iron. Metal-binding component (Potential) [H]

COG id: COG0803

COG function: function code P; ABC-type metal ion transport system, periplasmic component/surface adhesin

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 9 family [H]

Homologues:

Organism=Escherichia coli, GI87081990, Length=251, Percent_Identity=21.9123505976096, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006127
- InterPro:   IPR006129
- InterPro:   IPR006128 [H]

Pfam domain/function: PF01297 SBP_bac_9 [H]

EC number: NA

Molecular weight: Translated: 33427; Mature: 33427

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSIKKVTMLLGGLALTCSIAFQASATEKFKVITTFTIIADMAKNVAGDAAEVSSITKPG
CCCHHHHHHHHHHHHHEEEEEEECCCCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHCCCC
AEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNGVPEVIVSSGVTPVGITEG
CHHHCCCCCCCHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCC
PYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQRNADTYKAKITQTLAPLRKQ
CCCCCCCCCCEECCCCCEEEEECCHHHHEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHH
ITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQVRKVVDIVKKNHIPA
HHHCCCCCEEEEECCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCE
VFSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPVPTYIDLLKVTTSTLVQGIKAG
EECCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
KREK
CCCC
>Mature Secondary Structure
MHSIKKVTMLLGGLALTCSIAFQASATEKFKVITTFTIIADMAKNVAGDAAEVSSITKPG
CCCHHHHHHHHHHHHHEEEEEEECCCCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHCCCC
AEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNGVPEVIVSSGVTPVGITEG
CHHHCCCCCCCHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCC
PYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQRNADTYKAKITQTLAPLRKQ
CCCCCCCCCCEECCCCCEEEEECCHHHHEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHH
ITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQVRKVVDIVKKNHIPA
HHHCCCCCEEEEECCCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCE
VFSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPVPTYIDLLKVTTSTLVQGIKAG
EECCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
KREK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Zn (II) [Periplasm]; H2O; ATP [C]

Specific reaction: Zn (II) [Periplasm] + H2O + ATP = Zn (II) [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 1556062; 10675023 [H]