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The map label for this gene is nhaB

Identifier: 218700233

GI number: 218700233

Start: 1966089

End: 1967630

Strand: Direct

Name: nhaB

Synonym: ECIAI39_1884

Alternate gene names: 218700233

Gene position: 1966089-1967630 (Clockwise)

Preceding gene: 218700231

Following gene: 218700234

Centisome position: 38.31

GC content: 51.69

Gene sequence:

>1542_bases
ATGGAGATCTCCTGGGGCCGTGCCCTATGGCGCAACTTTTTGGGCCAGTCCCCCGACTGGTACAAACTCGCCCTCATTAT
TTTCTTAATTCTTAATCCGTTAATTTTCATCATCAGCCCCTTTGTTGCCGGTTGGCTGCTGGTGGCGGAATTTATTTTCA
CTCTGGCGATGGCCCTCAAATGCTATCCGCTGCTCCCCGGTGGTCTGTTGGCTATCGAAGCGGTATTCATCGGCATGACC
AGCGCGGAACATGTTCGTGAAGAGGTGGCGGCGAATCTTGAAGTCTTGCTGTTACTGATGTTTATGGTGGCGGGTATCTA
TTTTATGAAACAGCTGTTGCTGTTCATATTTACCCGTTTGCTGCTAAGCATTCGCTCCAAAATGCTGCTGTCGCTCTCTT
TTTGCATGGCGGCGGCGTTCCTCTCCGCGTTCCTCGATGCCTTAACCGTCGTGGCGGTGGTGATCAGCGTCGCAGTCGGT
TTTTATGGTATTTATCATCGTGTTGCCTCTTCCCGTACCGAAGATACCGACCTGCAAGACGATAGTCATATCGACAAGCA
TTACAAAGTGGTTCTGGAACAGTTCCGTGGCTTTTTGCGCAGCCTGATGATGCATGCAGGTGTCGGCACCGCATTAGGCG
GCGTGATGACCATGGTGGGCGAACCACAGAACTTGATCATCGCTAAAGCAGCTGGCTGGCATTTTGGCGATTTCTTCCTG
CGCATGTCGCCAGTGACCGTTCCGGTTCTGATTTGTGGCCTGTTGACCTGCCTACTGGTGGAGAAGCTGCGTTGTTTTGG
CTACGGCGAAACGCTGCCGGAGAAAGTCCGCGAAGTGCTGCAACAGTTTGACGATCAAAGTCGCCAACAGCGTACCCGTC
AGGATAAAATTCGTCTGATTGTCCAGGCGATTATCGGCGTCTGGCTGGTCACCGCGCTGGCGTTGCATCTGGCGGAGGTT
GGCCTGATTGGTTTGTCGGTCATTATTCTGGCGACATCATTGACTGGTGTCACTGATGAACATGCTATCGGTAAAGCCTT
CACCGAATCTCTGCCATTCACCGCACTGTTGACTGTCTTTTTCTCGGTCGTCGCGGTAATTATTGATCAGCAACTGTTCT
CACCAATCATTCATTTTGTCCTACAGGCATCGGAACACGCCCAGCTGTCGCTGTTCTATATTTTCAACGGTCTGCTGTCA
TCCATTTCGGATAACGTCTTCGTGGGGACGATTTATATCAACGAAGCGAAAGCGGCAATGGAAAGTGGCGCTATCACGTT
GAAGCAATACGAGCTGCTGGCGGTCGCCATTAATACCGGTACCAATCTGCCCTCTGTCGCTACGCCGAACGGTCAGGCGG
CGTTCTTGTTCCTGCTAACCTCCGCACTCGCGCCATTGATTCGTCTCTCTTATGGTCGCATGGTATGGATGGCTCTTCCC
TACACCCTCGTACTAACGGTTGTCGGGTTACTCTGTGTTGAGTTTACGCTTGCCCCTGTAACCGAATGGTTTATGCAAAT
GGGCTGGATAGCAACGCTTTGA

Upstream 100 bases:

>100_bases
TCCAGACCATCAACACTTGCCTCGATCCCCTCCTGACCGTTAAGCTTTGCGTTCTAAGATAAAAACATCATTGATTATTT
CTAAGGTAAGGGAAACCATC

Downstream 100 bases:

>100_bases
TAACAAATTACCGGGCATATAAATGCCCGGTTTGTCTTTTCGCCCGATAATTGTCCAATTGCGTCCTATTTTTGCTGCCT
CCTGGTGGCGCAGCGAATGA

Product: sodium/proton antiporter

Products: Na (I) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: Sodium/proton antiporter nhaB [H]

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MEISWGRALWRNFLGQSPDWYKLALIIFLILNPLIFIISPFVAGWLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMT
SAEHVREEVAANLEVLLLLMFMVAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCMAAAFLSAFLDALTVVAVVISVAVG
FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTMVGEPQNLIIAKAAGWHFGDFFL
RMSPVTVPVLICGLLTCLLVEKLRCFGYGETLPEKVREVLQQFDDQSRQQRTRQDKIRLIVQAIIGVWLVTALALHLAEV
GLIGLSVIILATSLTGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIHFVLQASEHAQLSLFYIFNGLLS
SISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPNGQAAFLFLLTSALAPLIRLSYGRMVWMALP
YTLVLTVVGLLCVEFTLAPVTEWFMQMGWIATL

Sequences:

>Translated_513_residues
MEISWGRALWRNFLGQSPDWYKLALIIFLILNPLIFIISPFVAGWLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMT
SAEHVREEVAANLEVLLLLMFMVAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCMAAAFLSAFLDALTVVAVVISVAVG
FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTMVGEPQNLIIAKAAGWHFGDFFL
RMSPVTVPVLICGLLTCLLVEKLRCFGYGETLPEKVREVLQQFDDQSRQQRTRQDKIRLIVQAIIGVWLVTALALHLAEV
GLIGLSVIILATSLTGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIHFVLQASEHAQLSLFYIFNGLLS
SISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPNGQAAFLFLLTSALAPLIRLSYGRMVWMALP
YTLVLTVVGLLCVEFTLAPVTEWFMQMGWIATL
>Mature_513_residues
MEISWGRALWRNFLGQSPDWYKLALIIFLILNPLIFIISPFVAGWLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMT
SAEHVREEVAANLEVLLLLMFMVAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCMAAAFLSAFLDALTVVAVVISVAVG
FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTMVGEPQNLIIAKAAGWHFGDFFL
RMSPVTVPVLICGLLTCLLVEKLRCFGYGETLPEKVREVLQQFDDQSRQQRTRQDKIRLIVQAIIGVWLVTALALHLAEV
GLIGLSVIILATSLTGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIHFVLQASEHAQLSLFYIFNGLLS
SISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPNGQAAFLFLLTSALAPLIRLSYGRMVWMALP
YTLVLTVVGLLCVEFTLAPVTEWFMQMGWIATL

Specific function: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons [H]

COG id: COG3067

COG function: function code P; Na+/H+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaB Na(+)/H(+) (TC 2.A.34) antiporter family [H]

Homologues:

Organism=Escherichia coli, GI1787435, Length=513, Percent_Identity=98.635477582846, Blast_Score=1017, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004671 [H]

Pfam domain/function: PF06450 NhaB [H]

EC number: NA

Molecular weight: Translated: 56678; Mature: 56678

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3 Proton [Periplasm]; Na (I) [Cytoplasm] [C]

Specific reaction: 3 Proton [Periplasm] + 2 Na (I) [Cytoplasm] = 2 Na (I) [Periplasm] + 3 Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA