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The map label for this gene is insF1 [C]

Identifier: 218700228

GI number: 218700228

Start: 1959658

End: 1960044

Strand: Direct

Name: insF1 [C]

Synonym: ECIAI39_1878

Alternate gene names: 218700228

Gene position: 1959658-1960044 (Clockwise)

Preceding gene: 218700227

Following gene: 218700231

Centisome position: 38.18

GC content: 52.71

Gene sequence:

>387_bases
ATGGAAACGACATTCGTGCTGGATGCACTGGAGCAGGCGTTATGGGCCCGTCGACCGTCCGGCACGGTCCATCACAGTGA
TAAAGGTTCTCAGTATGTATCGCTGGCCTACACACAGCGGCTTAAGGAAGCCGGATTACTGGCATCAACAGGAAGTACAG
GCGACTCGTATGACAACGCGATGGCGGAGAGCATCAATGGTCTTTACAAAGCGGAGGTAATACACCGTAAGAGCTGGAAA
AACCGTGCAGAAGTGGAACTGGCCACACTCACGTGGGGGGACTGGTATAACAATCGACGATTGCTGGAAAGGCTGGGCCA
TACTCCTCCGGCAGAAGCAGAAAAAGCTTATTATGCTTCCATCGGAAACGATGATCTGGCAGCCTGA

Upstream 100 bases:

>100_bases
GCCTCTTACTCTACTCAGCACATGGCGGGGCTTCGTCTATGTGGCGTTCATCATTGATGTGTTTGCCGGATACATCGTGG
GGTGACGGGTCTCATCGTCC

Downstream 100 bases:

>100_bases
GTTCACAGATAAAACACTCTCCAGGAAACCCGGGGCGGTTCACTGTATACACTGCGCTGTGCTTTCAACAGTATTAGCTT
AATACCTGATGAGATGCTGG

Product: transposase ORF B (fragment), IS629

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 128; Mature: 128

Protein sequence:

>128_residues
METTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWK
NRAEVELATLTWGDWYNNRRLLERLGHTPPAEAEKAYYASIGNDDLAA

Sequences:

>Translated_128_residues
METTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWK
NRAEVELATLTWGDWYNNRRLLERLGHTPPAEAEKAYYASIGNDDLAA
>Mature_128_residues
METTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWK
NRAEVELATLTWGDWYNNRRLLERLGHTPPAEAEKAYYASIGNDDLAA

Specific function: Involved in the transposition of the insertion sequence [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=91, Percent_Identity=56.043956043956, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1788405, Length=91, Percent_Identity=39.5604395604396, Blast_Score=64, Evalue=4e-12,
Organism=Escherichia coli, GI1787263, Length=91, Percent_Identity=39.5604395604396, Blast_Score=64, Evalue=4e-12,
Organism=Escherichia coli, GI1786753, Length=91, Percent_Identity=39.5604395604396, Blast_Score=64, Evalue=4e-12,
Organism=Escherichia coli, GI1786570, Length=91, Percent_Identity=39.5604395604396, Blast_Score=64, Evalue=4e-12,
Organism=Escherichia coli, GI1786490, Length=91, Percent_Identity=39.5604395604396, Blast_Score=64, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 14261; Mature: 14261

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNA
CCCHHHHHHHHHHHHHCCCCCCEEECCCCCEEEHHHHHHHHHHCCCEECCCCCCCCHHHH
MAESINGLYKAEVIHRKSWKNRAEVELATLTWGDWYNNRRLLERLGHTPPAEAEKAYYAS
HHHHHCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHCCCCCCCCCHHEEEC
IGNDDLAA
CCCCCCCC
>Mature Secondary Structure
METTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNA
CCCHHHHHHHHHHHHHCCCCCCEEECCCCCEEEHHHHHHHHHHCCCEECCCCCCCCHHHH
MAESINGLYKAEVIHRKSWKNRAEVELATLTWGDWYNNRRLLERLGHTPPAEAEKAYYAS
HHHHHCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHCCCCCCCCCHHEEEC
IGNDDLAA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2159625; 2824781 [H]