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The map label for this gene is insF1 [C]

Identifier: 218700073

GI number: 218700073

Start: 1779612

End: 1780058

Strand: Direct

Name: insF1 [C]

Synonym: ECIAI39_1704

Alternate gene names: 218700073

Gene position: 1779612-1780058 (Clockwise)

Preceding gene: 218700072

Following gene: 218700075

Centisome position: 34.68

GC content: 53.02

Gene sequence:

>447_bases
GTGGCGTTCATCATTGATGTGTTTGCCGGATACATCGTGGGGTGGCGGGTCTCATCGTCCATGGAAACGACATTCGTGCT
GGATGCACTGGAGCAGGCGTTATGGGCCCGTCGACCGTCCGGCACGGTCCATCACAGTGATAAAGGTTCTCAGTATGTAT
CGCTGGCCTACACACAGCGGCTTAAGGAAGCCGGATTACTGGCATCAACAGGAAGTACAGGCGACTCGTATGACAACGCG
ATGGCGGAGAGCATCAATGGTCTTTACAAAGCGGAGGTAATACACCGTAAGAGCTGGAAAAACCGTGCAGAAGTGGAACT
GGCCACACTCACGTGGGTGGACTGGTATAACAATCGACGATTGCTGGAAAGGCTGGGCCATACTCCTCCGGCAGAAGCAG
AAAAAGCTTATTATGCTTCCATCGGAAACGATGATCTGGCAGCCTGA

Upstream 100 bases:

>100_bases
GCTGAAAGGTGCACGTCCGGTTCGGGGAGGAGAGGCAGGGAAATAGTCCGACTACGCCCTGCCTCTTACTCTACTCAGCA
CATGGCGGGGCTTCGTCTAT

Downstream 100 bases:

>100_bases
GTTCACAGATAAAACACTCTCCAGGAAACCCGGGGCGGTTCATCCCGCCATCAGCAGGCTTAGCGCAACAAACGCGGCTA
CTTTCTTCATTGTGGTTCTC

Product: transposase ORF B (fragment), IS629

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MAFIIDVFAGYIVGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNA
MAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKAYYASIGNDDLAA

Sequences:

>Translated_148_residues
MAFIIDVFAGYIVGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNA
MAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKAYYASIGNDDLAA
>Mature_147_residues
AFIIDVFAGYIVGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDNAM
AESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKAYYASIGNDDLAA

Specific function: Involved in the transposition of the insertion sequence [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=110, Percent_Identity=57.2727272727273, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1788405, Length=136, Percent_Identity=33.0882352941176, Blast_Score=81, Evalue=3e-17,
Organism=Escherichia coli, GI1787263, Length=136, Percent_Identity=33.0882352941176, Blast_Score=81, Evalue=3e-17,
Organism=Escherichia coli, GI1786753, Length=136, Percent_Identity=33.0882352941176, Blast_Score=81, Evalue=3e-17,
Organism=Escherichia coli, GI1786570, Length=136, Percent_Identity=33.0882352941176, Blast_Score=81, Evalue=3e-17,
Organism=Escherichia coli, GI1786490, Length=136, Percent_Identity=33.0882352941176, Blast_Score=81, Evalue=3e-17,
Organism=Escherichia coli, GI1789981, Length=139, Percent_Identity=29.4964028776978, Blast_Score=71, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 16504; Mature: 16372

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFIIDVFAGYIVGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQR
CEEHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEHHHHHHH
LKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRR
HHHCCCEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEEEEEHHHCCHH
LLERLGHTPPAEAEKAYYASIGNDDLAA
HHHHCCCCCCCCCCHHEEEECCCCCCCC
>Mature Secondary Structure 
AFIIDVFAGYIVGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQR
EEHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEHHHHHHH
LKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRR
HHHCCCEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEEEEEHHHCCHH
LLERLGHTPPAEAEKAYYASIGNDDLAA
HHHHCCCCCCCCCCHHEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2159625; 2824781 [H]