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The map label for this gene is pfkB

Identifier: 218699710

GI number: 218699710

Start: 1396012

End: 1396941

Strand: Reverse

Name: pfkB

Synonym: ECIAI39_1331

Alternate gene names: 218699710

Gene position: 1396941-1396012 (Counterclockwise)

Preceding gene: 218699712

Following gene: 218699709

Centisome position: 27.22

GC content: 53.44

Gene sequence:

>930_bases
ATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAA
GCTGCGCTGTACCGCACCGGTATTCGAACCCGGAGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCA
GTGCCACAGCGATCTTCCCGGCGGGTGGTGCGACCGGCGAACATCTGGTTTCACTGTTGGCAGATGAAAATGTCCCCGTC
GCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAGCAGTATCGTTTTGT
CATGCCAGGCGCGGCATTAAGTGAAGATGAGTTTCGCCAGCTTGAAGAGCAAGTTCTGGAAATTGAATCCGGTGCTATCC
TGGTCATAAGCGGAAGCCTGCCGCCAGGTGTGAAGCTGGAAAAATTAACCCAACTGATTTCCGCTGCGCAAAAACAAGGG
ATTCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCGATTGGTAATATCGAGTTGGTTAAGCCTAA
CCAAAAAGAACTCAGTGCGCTAGTGAATCGCGAACTCACCCAGCCAGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTA
ATAGCGGCAAGGCCAAACGAGTTGTCGTTTCCCTGGGTCCACAAGGGGCGTTGGGCGTTGATAGTGAAAACTGCATTCAG
GTTGTGCCACCACCGGTGAAAAGCCAGAGCACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGC
AGAAAATGCCTCTCTTAAAGAGATGGTCCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGCC
TGTGCTCCCATGACGATACACAAAAAATTTACGCTTACCTTTCCCGCTAA

Upstream 100 bases:

>100_bases
TCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAA
TCAGCCTATAGGAGGAAATG

Downstream 100 bases:

>100_bases
CTGAAACATTCCCCCTGCATTGGGGGAATCACCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATG
TGATAACAAAGGGGTGAACT

Product: 6-phosphofructokinase 2

Products: NA

Alternate protein names: Phosphofructokinase-2 [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINVARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPV
ATVEAKDWTRQNLHVHVEASGEQYRFVMPGAALSEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKAKRVVVSLGPQGALGVDSENCIQ
VVPPPVKSQSTVGAGDSMVGAMTLKLAENASLKEMVRFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR

Sequences:

>Translated_309_residues
MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINVARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPV
ATVEAKDWTRQNLHVHVEASGEQYRFVMPGAALSEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKAKRVVVSLGPQGALGVDSENCIQ
VVPPPVKSQSTVGAGDSMVGAMTLKLAENASLKEMVRFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR
>Mature_309_residues
MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINVARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPV
ATVEAKDWTRQNLHVHVEASGEQYRFVMPGAALSEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKAKRVVVSLGPQGALGVDSENCIQ
VVPPPVKSQSTVGAGDSMVGAMTLKLAENASLKEMVRFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR

Specific function: Key control step of glycolysis. [C]

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788017, Length=309, Percent_Identity=99.3527508090615, Blast_Score=623, Evalue=1e-180,
Organism=Escherichia coli, GI1788493, Length=284, Percent_Identity=33.4507042253521, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: 496 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 32428; Mature: 32428

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00583 PFKB_KINASES_1 ; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINVARAIAHLGGSATAIFP
CEEEEEEEECCCCCCCEECCEECCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEE
AGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGEQYRFVMPGAALSEDEFRQ
CCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEECCCEEEEEECCCCCCHHHHHH
LEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQGIRCIVDSSGEALSAALAIGN
HHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEECC
IELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKAKRVVVSLGPQGALGVDSENCIQ
EEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEE
VVPPPVKSQSTVGAGDSMVGAMTLKLAENASLKEMVRFGVAAGSAATLNQGTRLCSHDDT
ECCCCCCCCCCCCCCCCHHEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCH
QKIYAYLSR
HHHHHHHCC
>Mature Secondary Structure
MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINVARAIAHLGGSATAIFP
CEEEEEEEECCCCCCCEECCEECCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEE
AGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGEQYRFVMPGAALSEDEFRQ
CCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEECCCEEEEEECCCCCCHHHHHH
LEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQGIRCIVDSSGEALSAALAIGN
HHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEECC
IELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKAKRVVVSLGPQGALGVDSENCIQ
EEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEE
VVPPPVKSQSTVGAGDSMVGAMTLKLAENASLKEMVRFGVAAGSAATLNQGTRLCSHDDT
ECCCCCCCCCCCCCCCCHHEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCH
QKIYAYLSR
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6235149; 9097039; 9278503; 6310120 [H]