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The map label for this gene is ntpA

Identifier: 218699563

GI number: 218699563

Start: 1255123

End: 1255566

Strand: Direct

Name: ntpA

Synonym: ECIAI39_1184

Alternate gene names: 218699563

Gene position: 1255123-1255566 (Clockwise)

Preceding gene: 218699562

Following gene: 218699564

Centisome position: 24.46

GC content: 52.93

Gene sequence:

>444_bases
ATGGCTTATAAGCGTCCCGTTTCGATCTTAGTGGTCATCTATGCACAAGATACGAAACGGGTGCTGATGTTGCAGCGGCG
TGACGATCCCGATTTCTGGCAGTCGGTAACCGGCAGCGTGGAAGAGGGTGAAACCGCGCCGCAAGCTGCCATGCGCGAAG
TAAAGGAAGAGGTCACCATCGATGTTGTCGCTGAACAACTGACCTTAATTGACTGTCAGCGCACGGTAGAATTTGAAATT
TTTTCACATTTACGTCATCGCTATGCGCCGGGCGTGACGCGTAATACGGAATCATGGTTCTGTCTTGCGCTCCCGCACGA
GCGCCAGATCGTTTTCACTGAACATCTGGCTTACAAGTGGCTTGATGCGCCTGCTGCGGCGGCGCTCACTAAGTCCTGGA
GCAACCGGCAGGCGATTGAACAGTTTGTAATTAACGCTGCCTGA

Upstream 100 bases:

>100_bases
GCGGCAGCGTGTCTGATGACTGAAGCACCGAGCTTTGCTAACCCGACTGCACTGGCTGAGCTGAGCATTCAGGTTGTGAA
GAAGGCTGAGAATAACTGAT

Downstream 100 bases:

>100_bases
ACAGGCAGGGCTATCTGGAGATATTTTTATGGCAGGTCATAGTAAATGGGCCAACACCAGACATCGTAAAGCTGCGCAGG
ATGCTAAGCGCGGTAAAATC

Product: dATP pyrophosphohydrolase

Products: dAMP; pyrophosphate [C]

Alternate protein names: dATP pyrophosphohydrolase [H]

Number of amino acids: Translated: 147; Mature: 146

Protein sequence:

>147_residues
MAYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVEEGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEI
FSHLRHRYAPGVTRNTESWFCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA

Sequences:

>Translated_147_residues
MAYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVEEGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEI
FSHLRHRYAPGVTRNTESWFCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA
>Mature_146_residues
AYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVEEGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIF
SHLRHRYAPGVTRNTESWFCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA

Specific function: Catalyzes the hydrolysis of dihydroneopterin triphosphate to dihydroneopterin monophosphate and pyrophosphate. Required for efficient folate biosynthesis. Can also hydrolyze nucleoside triphosphates with a preference for dATP [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788172, Length=145, Percent_Identity=100, Blast_Score=301, Evalue=8e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003564
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 16906; Mature: 16775

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVEEGETAPQAAMREVKEEVTI
CCCCCCEEEEEEEEECCCCEEEEEECCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
DVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESWFCLALPHERQIVFTEHLAYKW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEEEEHHHHHHHH
LDAPAAAALTKSWSNRQAIEQFVINAA
HCCCHHHHHHHCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
AYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVEEGETAPQAAMREVKEEVTI
CCCCCEEEEEEEEECCCCEEEEEECCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
DVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESWFCLALPHERQIVFTEHLAYKW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEEEEHHHHHHHH
LDAPAAAALTKSWSNRQAIEQFVINAA
HCCCHHHHHHHCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxy-ATP; H2O [C]

Specific reaction: deoxy-ATP + H2O = dAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]