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The map label for this gene is zitB

Identifier: 218699117

GI number: 218699117

Start: 762719

End: 763660

Strand: Reverse

Name: zitB

Synonym: ECIAI39_0720

Alternate gene names: 218699117

Gene position: 763660-762719 (Counterclockwise)

Preceding gene: 218699118

Following gene: 218699104

Centisome position: 14.88

GC content: 52.87

Gene sequence:

>942_bases
ATGGCGCACTCACACTCACACACTTCCTCACACCTGCCAGAAGATAATAATGCTCGTCGCTTGTTGTATGCTTTCGGCGT
GACTGCCGGGTTTATGCTCGTAGAAGTCATTGGTGGTTTTCTTTCCGGTTCTCTGGCATTGCTGGCCGATGCGGGTCATA
TGTTGACCGATACTGCCGCCCTGCTTTTCGCCCTACTCGCAGTGCAATTTTCCCGTCGTCCTCCCACCATTCGCCACACT
TTCGGTTGGCTAAGATTGACCACGCTCGCGGCGTTTGTGAATGCCATCGCCCTGGTGGTGATTACTATTTTGATTGTCTG
GGAGGCTATAGAACGCTTCCGCACGCCGCGTCCGGTCGAGGGCGGCATGATGATGGCAATTGCCGTGGCTGGATTGCTGG
CAAATATACTTTCTTTCTGGTTACTTCATCACGGCAGCGAAGAGAAAAACCTCAACGTGCGAGCAGCGGCACTGCATGTG
CTCGGGGACCTGCTGGGTTCGGTTGGGGCTATTATTGCCGCGTTGATTATTATCTGGACCGGCTGGACACCTGCTGACCC
CATTCTCTCAATACTGGTGTCGCTTCTGGTTCTGCGTAGCGCGTGGCGACTGTTGAAAGATAGCGTGAATGAATTACTTG
AAGGTGCACCGGTATCGCTGGATATCGCCGAACTGAAGCGTCGTATGTGCCGGGAAATCCCGGAAGTCCGCAATGTGCAC
CATGTACATGTATGGATGGTAGGCGAGAAGCCGGTGATGACGCTGCATGTGCAGGTGATCCCGCCACACGATCACGATGC
CTTGTTAGATCAGATCCAACACTACCTGATGGATCACTATCAGATTGAGCACGCCACTATTCAGATGGAATATCAACCTT
GTCATGGGCCAGACTGCCACCTGAATGAAGGGGTGTCTGGCCATTCACATCATCACCATTAA

Upstream 100 bases:

>100_bases
CCAATAATGGGAGAGCGGAGTCTCTCCCTTTTAATTTTTCCCTTCTAAAAGCAGTATGATGTCATAAAGTTAAGTGAGAA
TAATTAAGGGATGACTCGTC

Downstream 100 bases:

>100_bases
TGGGACAGCGCGCGTGAACCTCTTTCGCGTGCGCTGTTGATCCACATCCGGCTGCCGTTGAGCGCAATAAAGGTCAGGAT
GATGTACTCCAGAGACATGG

Product: zinc transporter ZitB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGSLALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHT
FGWLRLTTLAAFVNAIALVVITILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPVSLDIAELKRRMCREIPEVRNVH
HVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYLMDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH

Sequences:

>Translated_313_residues
MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGSLALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHT
FGWLRLTTLAAFVNAIALVVITILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPVSLDIAELKRRMCREIPEVRNVH
HVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYLMDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH
>Mature_312_residues
AHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGSLALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTF
GWLRLTTLAAFVNAIALVVITILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHVL
GDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPVSLDIAELKRRMCREIPEVRNVHH
VHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYLMDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH

Specific function: Involved in zinc efflux across the cytoplasmic membrane, thus reducing zinc accumulation in the cytoplasm and rendering bacteria more resistant to zinc. It may contribute to zinc homeostasis at low concentrations of zinc, whereas zntA is required for grow

COG id: COG1230

COG function: function code P; Co/Zn/Cd efflux system component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily [H]

Homologues:

Organism=Homo sapiens, GI52352805, Length=320, Percent_Identity=32.1875, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI289803013, Length=320, Percent_Identity=32.5, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI289803009, Length=320, Percent_Identity=32.5, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI289803007, Length=320, Percent_Identity=32.5, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI289803003, Length=320, Percent_Identity=32.5, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI64762489, Length=320, Percent_Identity=32.5, Blast_Score=148, Evalue=5e-36,
Organism=Homo sapiens, GI21361112, Length=309, Percent_Identity=33.6569579288026, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI21359890, Length=289, Percent_Identity=31.4878892733564, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI20070323, Length=348, Percent_Identity=25.2873563218391, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI14210520, Length=231, Percent_Identity=29.004329004329, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI52352803, Length=132, Percent_Identity=43.1818181818182, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI52351208, Length=162, Percent_Identity=29.6296296296296, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI222080086, Length=129, Percent_Identity=27.1317829457364, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI40255009, Length=129, Percent_Identity=27.1317829457364, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1786966, Length=313, Percent_Identity=99.6805111821086, Blast_Score=630, Evalue=0.0,
Organism=Escherichia coli, GI1790349, Length=276, Percent_Identity=23.9130434782609, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI193210990, Length=321, Percent_Identity=33.3333333333333, Blast_Score=166, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17555794, Length=312, Percent_Identity=32.6923076923077, Blast_Score=154, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI17555796, Length=310, Percent_Identity=32.258064516129, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI32564488, Length=303, Percent_Identity=31.023102310231, Blast_Score=134, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17550440, Length=129, Percent_Identity=32.5581395348837, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17550442, Length=129, Percent_Identity=32.5581395348837, Blast_Score=74, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6320411, Length=179, Percent_Identity=35.7541899441341, Blast_Score=93, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6323899, Length=132, Percent_Identity=36.3636363636364, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324892, Length=120, Percent_Identity=37.5, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24656728, Length=346, Percent_Identity=29.4797687861272, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24656724, Length=346, Percent_Identity=29.4797687861272, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24656720, Length=346, Percent_Identity=29.4797687861272, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24656715, Length=346, Percent_Identity=29.4797687861272, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI21357035, Length=346, Percent_Identity=29.4797687861272, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24583858, Length=321, Percent_Identity=29.2834890965732, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24585900, Length=125, Percent_Identity=32.8, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24585898, Length=125, Percent_Identity=32.8, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24585896, Length=125, Percent_Identity=32.8, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI19921672, Length=125, Percent_Identity=32.8, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24584409, Length=157, Percent_Identity=30.5732484076433, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI28574430, Length=157, Percent_Identity=30.5732484076433, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24645863, Length=127, Percent_Identity=30.7086614173228, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24667550, Length=290, Percent_Identity=23.7931034482759, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24667546, Length=290, Percent_Identity=23.7931034482759, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002524 [H]

Pfam domain/function: PF01545 Cation_efflux [H]

EC number: NA

Molecular weight: Translated: 34693; Mature: 34562

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: PS00511 CRF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGSLALLADAGHMLTDTAA
CCCCCCCCHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHH
LLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVITILIVWEAIERFRTPRPVE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHVLGDLLGSVGAIIAALIIIWT
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPVSLDIAELKRRMCREIPEVRNVH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCE
HVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYLMDHYQIEHATIQMEYQPCHGPDCH
EEEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCC
LNEGVSGHSHHHH
CCCCCCCCCCCCC
>Mature Secondary Structure 
AHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGSLALLADAGHMLTDTAA
CCCCCCCHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHH
LLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVITILIVWEAIERFRTPRPVE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHVLGDLLGSVGAIIAALIIIWT
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPVSLDIAELKRRMCREIPEVRNVH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCE
HVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYLMDHYQIEHATIQMEYQPCHGPDCH
EEEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCC
LNEGVSGHSHHHH
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 11443104 [H]