Click here to switch to the map view.

The map label for this gene is vpeA

Identifier: 218698116

GI number: 218698116

Start: 5022380

End: 5022823

Strand: Direct

Name: vpeA

Synonym: EC55989_4926

Alternate gene names: 218698116

Gene position: 5022380-5022823 (Clockwise)

Preceding gene: 218698112

Following gene: 218698117

Centisome position: 97.43

GC content: 47.97

Gene sequence:

>444_bases
ATGTTAAAAAATCTATTAAATACAGAGGTCGTTCAGGTCGTGGAACAGGCCAAAGACTGGCGGGAAGCCGTGGCGATTTC
ATGCCGGCCGTTGATTGAAAACGGTTCTATCGAACCGCGTTATGTTGATGCGATCTATCGCTCGCACGACACTATCGGTC
CGTACTATGTGGTTGGCCCTGGTATTGCGATGCCTCATGCGCGCCCGGAAGAGGGAGCTAACAAGCTGTCATTAGCACTG
ACCCTGATCCCTTCAGGTGTGAATTTTGATGCCGATGAAAACGATCCAGTGAAATTACTGATTGTGCTGGCTGCAACCGA
CAGTACCAGCCACATTGAAGCGATTTCTCAACTCGCCAAGTTATTCGATAACGAAAAAGACATACAGGCCATTTTGATGG
CCAAAACGACGCAGGACATTTTGTCTGTTATTGCGCGCTATTAA

Upstream 100 bases:

>100_bases
AGCGTTATCAGATAGCGCTATCAAATGCGACAATCATCACATTAATTAATGCGTAAAATATCTACTCTCCTGTTAATTAT
CATCGGGAGTTATCAGTAGC

Downstream 100 bases:

>100_bases
TCCAGAGGAACATTTATGAAAATTACAGTGGTTTGCGGTAACGGCTTAGGCACCAGCCTGATGATGGAAATGAGCATTAA
AAGCATTTTGAAGGATTTGT

Product: Putative PTS system, specific IIA component

Products: protein histidine; sugar phosphate

Alternate protein names: Putative PTS system EIIA component [H]

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MLKNLLNTEVVQVVEQAKDWREAVAISCRPLIENGSIEPRYVDAIYRSHDTIGPYYVVGPGIAMPHARPEEGANKLSLAL
TLIPSGVNFDADENDPVKLLIVLAATDSTSHIEAISQLAKLFDNEKDIQAILMAKTTQDILSVIARY

Sequences:

>Translated_147_residues
MLKNLLNTEVVQVVEQAKDWREAVAISCRPLIENGSIEPRYVDAIYRSHDTIGPYYVVGPGIAMPHARPEEGANKLSLAL
TLIPSGVNFDADENDPVKLLIVLAATDSTSHIEAISQLAKLFDNEKDIQAILMAKTTQDILSVIARY
>Mature_147_residues
MLKNLLNTEVVQVVEQAKDWREAVAISCRPLIENGSIEPRYVDAIYRSHDTIGPYYVVGPGIAMPHARPEEGANKLSLAL
TLIPSGVNFDADENDPVKLLIVLAATDSTSHIEAISQLAKLFDNEKDIQAILMAKTTQDILSVIARY

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790755, Length=135, Percent_Identity=39.2592592592593, Blast_Score=117, Evalue=3e-28,
Organism=Escherichia coli, GI2367359, Length=144, Percent_Identity=37.5, Blast_Score=117, Evalue=5e-28,
Organism=Escherichia coli, GI1789302, Length=145, Percent_Identity=40, Blast_Score=101, Evalue=2e-23,
Organism=Escherichia coli, GI1790027, Length=142, Percent_Identity=28.169014084507, Blast_Score=59, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16158; Mature: 16158

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKNLLNTEVVQVVEQAKDWREAVAISCRPLIENGSIEPRYVDAIYRSHDTIGPYYVVGP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCCCCCHHHHHHHHHCCCCCCCEEEECC
GIAMPHARPEEGANKLSLALTLIPSGVNFDADENDPVKLLIVLAATDSTSHIEAISQLAK
CEECCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHH
LFDNEKDIQAILMAKTTQDILSVIARY
HHCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKNLLNTEVVQVVEQAKDWREAVAISCRPLIENGSIEPRYVDAIYRSHDTIGPYYVVGP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCCCCCHHHHHHHHHCCCCCCCEEEECC
GIAMPHARPEEGANKLSLALTLIPSGVNFDADENDPVKLLIVLAATDSTSHIEAISQLAK
CEECCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHH
LFDNEKDIQAILMAKTTQDILSVIARY
HHCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]