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The map label for this gene is cycA

Identifier: 218697961

GI number: 218697961

Start: 4877773

End: 4879185

Strand: Direct

Name: cycA

Synonym: EC55989_4768

Alternate gene names: 218697961

Gene position: 4877773-4879185 (Clockwise)

Preceding gene: 218697960

Following gene: 218697965

Centisome position: 94.62

GC content: 52.23

Gene sequence:

>1413_bases
ATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACA
TATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGC
CGTCGATCATTTTCGTTTATATGATCATCGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCG
AATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTA
CTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACTGCTTATGCTCAGTTCTGGTTCCCCGATCTCT
CCGACTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATG
GAGTTCTGGTTTGCGATGATCAAAATCGTCGCCATTGTGTCGCTGATTGTCGTCGGCCTGGTTATGGTGGCGATGCACTT
TCAGTCACCGACTGGTGTGGAAGCGTCATTCGCGCATTTGTGGAATGATGGCGGCTGGTTCCCGAAAGGTTTAAGTGGCT
TCTTTGCCGGATTCCAGATAGCGGTTTTCGCTTTCGTAGGGATTGAGCTGGTAGGTACAACCGCTGCGGAAACCAAAGAT
CCGGAGAAATCACTGCCGCGTGCAATTAACTCTATTCCGATCCGTATCATTATGTTCTACGTCTTCGCGCTGATTGTAAT
TATGTCCGTGACGCCGTGGAGTTCGGTAGTCCCGGAGAAAAGCCCGTTTGTTGAACTGTTCGTGTTGGTAGGGCTGCCTG
CTGCCGCAAGCGTGATCAACTTTGTGGTGCTGACCTCTGCGGCGTCTTCCGCTAACAGCGGCGTCTTCTCTACCAGCCGT
ATGCTGTTTGGTCTGGCGCAGGAAGGTGTAGCACCGAAAGCGTTCGCTAAACTTTCTAAGCGCGCAGTACCCGCGAAAGG
GTTGACCTTCTCGTGTATCTGTCTGCTCGGCGGCGTGGTGATGTTGTATGTGAATCCTAGCGTGATTGGCGCGTTCACGA
TGATTACAACCGTTTCCGCGATTCTGTTTATGTTCGTCTGGACAATTATCCTTTGCTCGTACCTGGTTTACCGCAAACAG
CGTCCTCATCTGCATGAGAAGTCGATCTACAAGATGCCGCTCGGCAAGCTGATGTGCTGGGTATGTATGGCGTTCTTTGT
GTTCGTGGTCGTGTTGCTGACACTGGAAGATGACACTCGCCAGGCGCTGTTGGTCACCCCGCTGTGGTTTATCGCACTGG
GGCTGGGCTGGTTGTTTATTGGTAAGAAGCGGGCTGCTGAACTGCGGAAATAA

Upstream 100 bases:

>100_bases
TACCTAATATCGATGAGTCCCGATACAGATTCGTCGTATCATAGACTGACTAAAGGCCGTAGAGCCTGAACAACACAGAC
AGGTACAGGAAGAAAAAAAC

Downstream 100 bases:

>100_bases
CCGCATTATCATGCTGGATGGCGCAATGCCATCCAGCTTTTAGATTACTCACCCGCCAGCGCGCGTGGGAACAGTACATT
GTTTTCCAGGCTGATGTGAT

Product: D-alanine/D-serine/glycine permease

Products: D-glycine [Cytoplasm]; D-alanine [Cytoplasm]; Proton [Cytoplasm]; D-serine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMGSGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLS
NLEYKSFSDFASDLLGPWAGYFTGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGFQIAVFAFVGIELVGTTAAETKD
PEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSVVPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSR
MLFGLAQEGVAPKAFAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILCSYLVYRKQ
RPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWFIALGLGWLFIGKKRAAELRK

Sequences:

>Translated_470_residues
MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMGSGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLS
NLEYKSFSDFASDLLGPWAGYFTGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGFQIAVFAFVGIELVGTTAAETKD
PEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSVVPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSR
MLFGLAQEGVAPKAFAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILCSYLVYRKQ
RPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWFIALGLGWLFIGKKRAAELRK
>Mature_470_residues
MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMGSGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLS
NLEYKSFSDFASDLLGPWAGYFTGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGFQIAVFAFVGIELVGTTAAETKD
PEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSVVPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSR
MLFGLAQEGVAPKAFAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILCSYLVYRKQ
RPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWFIALGLGWLFIGKKRAAELRK

Specific function: Permease that is involved in the transport across the cytoplasmic membrane of D-alanine, D-serine and glycine

COG id: COG1113

COG function: function code E; Gamma-aminobutyrate permease and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid transporter (AAT) (TC 2.A.3.1) family

Homologues:

Organism=Homo sapiens, GI110347453, Length=367, Percent_Identity=22.0708446866485, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI114326544, Length=363, Percent_Identity=22.038567493113, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI114326550, Length=363, Percent_Identity=22.038567493113, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI181337167, Length=343, Percent_Identity=22.1574344023324, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI115648063, Length=369, Percent_Identity=25.4742547425474, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI115648022, Length=369, Percent_Identity=25.4742547425474, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI258645169, Length=145, Percent_Identity=29.6551724137931, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1790653, Length=470, Percent_Identity=100, Blast_Score=944, Evalue=0.0,
Organism=Escherichia coli, GI1786302, Length=461, Percent_Identity=40.3470715835141, Blast_Score=351, Evalue=6e-98,
Organism=Escherichia coli, GI1786602, Length=454, Percent_Identity=41.1894273127753, Blast_Score=337, Evalue=7e-94,
Organism=Escherichia coli, GI1786789, Length=453, Percent_Identity=37.9690949227373, Blast_Score=337, Evalue=9e-94,
Organism=Escherichia coli, GI48994972, Length=462, Percent_Identity=38.5281385281385, Blast_Score=325, Evalue=4e-90,
Organism=Escherichia coli, GI87081915, Length=459, Percent_Identity=37.2549019607843, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI87081708, Length=465, Percent_Identity=32.9032258064516, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1788480, Length=422, Percent_Identity=35.0710900473934, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI1789017, Length=447, Percent_Identity=29.9776286353468, Blast_Score=225, Evalue=5e-60,
Organism=Escherichia coli, GI2367353, Length=399, Percent_Identity=24.3107769423559, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082023, Length=351, Percent_Identity=21.6524216524217, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17532491, Length=365, Percent_Identity=24.3835616438356, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17531343, Length=449, Percent_Identity=21.826280623608, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17540018, Length=374, Percent_Identity=25.9358288770053, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6324061, Length=461, Percent_Identity=32.1041214750542, Blast_Score=206, Evalue=5e-54,
Organism=Saccharomyces cerevisiae, GI6324924, Length=426, Percent_Identity=31.2206572769953, Blast_Score=193, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6320772, Length=446, Percent_Identity=29.1479820627803, Blast_Score=183, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6322892, Length=421, Percent_Identity=29.6912114014252, Blast_Score=167, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6324990, Length=414, Percent_Identity=30.4347826086957, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6324059, Length=459, Percent_Identity=28.7581699346405, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6324553, Length=399, Percent_Identity=28.0701754385965, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6321629, Length=430, Percent_Identity=27.6744186046512, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6320717, Length=414, Percent_Identity=25.8454106280193, Blast_Score=149, Evalue=7e-37,
Organism=Saccharomyces cerevisiae, GI6319824, Length=408, Percent_Identity=25.9803921568627, Blast_Score=146, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6319543, Length=437, Percent_Identity=27.0022883295195, Blast_Score=146, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6320251, Length=402, Percent_Identity=26.865671641791, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6321053, Length=410, Percent_Identity=26.8292682926829, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6319608, Length=417, Percent_Identity=26.6187050359712, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6319542, Length=400, Percent_Identity=25.75, Blast_Score=144, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6322967, Length=422, Percent_Identity=28.9099526066351, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324981, Length=419, Percent_Identity=27.4463007159904, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6320364, Length=489, Percent_Identity=24.1308793456033, Blast_Score=94, Evalue=5e-20,
Organism=Drosophila melanogaster, GI221512776, Length=358, Percent_Identity=21.2290502793296, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24666159, Length=358, Percent_Identity=21.2290502793296, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI45550968, Length=419, Percent_Identity=26.0143198090692, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI19921172, Length=419, Percent_Identity=26.0143198090692, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYCA_ECO57 (P0AAE1)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B86118
- PIR:   B91277
- RefSeq:   NP_290840.1
- RefSeq:   NP_313213.1
- ProteinModelPortal:   P0AAE1
- EnsemblBacteria:   EBESCT00000024522
- EnsemblBacteria:   EBESCT00000057756
- GeneID:   913962
- GeneID:   959913
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5819
- KEGG:   ecs:ECs5186
- GeneTree:   EBGT00050000009004
- HOGENOM:   HBG492579
- OMA:   LMVTPLW
- ProtClustDB:   PRK11049
- BioCyc:   ECOL83334:ECS5186-MONOMER
- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004840
- PANTHER:   PTHR11785
- PIRSF:   PIRSF006060

Pfam domain/function: PF00324 AA_permease

EC number: NA

Molecular weight: Translated: 51660; Mature: 51660

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS00218 AMINO_ACID_PERMEASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1e04a80c)-; HASH(0x1853d9a4)-; HASH(0x1e1bc0f0)-; HASH(0x1ec966d0)-; HASH(0x1e56ad6c)-; HASH(0x1ecab300)-; HASH(0x1ec967fc)-; HASH(0x1e2f8628)-; HASH(0x1ec0f090)-; HASH(0x1eae8a0c)-; HASH(0x1e8a7f18)-; HASH(0x1ea8555c)-;

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMGSGKTISLAGPSIIFVY
CCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEECCHHHHHEEECCCCEEEECCHHHHHHH
MIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYFTGWTYWFCWVVTGMADVV
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEMEFWFAMIKIVAIVSLIVVGL
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGFQIAVFAFVGIELVGTTAAETKD
HHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSVVPEKSPFVELFVLVGLPAAASVIN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHCCCHHHHHHH
FVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKAFAKLSKRAVPAKGLTFSCICLLGGVV
HHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
MLYVNPSVIGAFTMITTVSAILFMFVWTIILCSYLVYRKQRPHLHEKSIYKMPLGKLMCW
HEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHH
VCMAFFVFVVVLLTLEDDTRQALLVTPLWFIALGLGWLFIGKKRAAELRK
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCH
>Mature Secondary Structure
MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMGSGKTISLAGPSIIFVY
CCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEECCHHHHHEEECCCCEEEECCHHHHHHH
MIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYFTGWTYWFCWVVTGMADVV
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEMEFWFAMIKIVAIVSLIVVGL
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGFQIAVFAFVGIELVGTTAAETKD
HHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSVVPEKSPFVELFVLVGLPAAASVIN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHCCCHHHHHHH
FVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKAFAKLSKRAVPAKGLTFSCICLLGGVV
HHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
MLYVNPSVIGAFTMITTVSAILFMFVWTIILCSYLVYRKQRPHLHEKSIYKMPLGKLMCW
HEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHH
VCMAFFVFVVVLLTLEDDTRQALLVTPLWFIALGLGWLFIGKKRAAELRK
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-glycine [Periplasm]; D-alanine [Periplasm]; Proton [Periplasm]; D-serine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + D-glycine [Periplasm] = Proton [Cytoplasm] + D-glycine [Cytoplasm] Proton [Periplasm] + D-alanine [Periplasm] = Proton [Cytoplasm] + D-alanine [Cytoplasm] Proton [Periplasm] + D-serine [Periplasm] = Proton [Cytoplasm] + D-serine [Cyto

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796