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The map label for this gene is uhpT

Identifier: 218697388

GI number: 218697388

Start: 4220797

End: 4222188

Strand: Reverse

Name: uhpT

Synonym: EC55989_4134

Alternate gene names: 218697388

Gene position: 4222188-4220797 (Counterclockwise)

Preceding gene: 218697389

Following gene: 218697386

Centisome position: 81.91

GC content: 53.02

Gene sequence:

>1392_bases
ATGCTGGCTTTCTTAAACCAGGTTCGCAAGCCGACCCTGGACCTTCCGCTCGAAGTGCGGCGCAAAATGTGGTTCAAACC
GTTCATGCAATCCTACCTGGTGGTCTTTATCGGCTACCTGACGATGTACCTGATTCGCAAGAACTTTAACATCGCGCAGA
ACGACATGATCTCGACCTACGGGTTGAGCATGACGCAGCTGGGGATGATCGGCCTGGGTTTCTCCATCACTTATGGCGTG
GGTAAAACGCTGGTTTCCTACTACGCCGACGGCAAAAACACCAAACAATTCCTGCCGTTCATGCTGATCCTGTCTGCCAT
TTGTATGCTGGGCTTCAGTGCCAGTATGGGCAGCGGCTCGGTTAGCCTGTTCCTGATGATTGCCTTCTACGCCTTAAGCG
GCTTTTTCCAGAGTACCGGCGGTTCGTGCAGTTACTCCACCATCACCAAATGGACGCCGCGTCGTAAACGCGGGACATTC
CTCGGTTTCTGGAATATTTCTCACAACCTTGGCGGTGCAGGCGCAGCAGGTGTGGCGCTGTTCGGGGCAAATTACCTGTT
CGATGGCCATGTCATCGGCATGTTTATCTTCCCGTCGATTATCGCGCTGATTGTCGGTTTTATCGGCCTGCGTTACGGCA
GCGACTCCCCGGAATCTTATGGCCTCGGCAAAGCTGAAGAACTGTTCGGCGAGGAGATCAGCGAAGAGGACAAAGAGACA
GAATCTACCGATATGACCAAGTGGCAGATCTTTGTTGAGTATGTGCTGAAAAACAAAGTGATCTGGCTGCTGTGCTTCGC
CAACATTTTCCTCTATGTGGTACGTATTGGTATCGACCAGTGGTCAACCGTATACGCGTTCCAGGAACTGAAACTCTCTA
AAGCGGTGGCGATTCAGGGCTTTACGCTGTTTGAAGCTGGTGCGCTGGTCGGTACGCTGCTGTGGGGCTGGCTCTCTGAC
CTGGCGAACGGTCGCCGTGGCCTGGTGGCCTGCATCGCGCTGGCGCTGATTATCGCCACGCTCGGTGTGTATCAACATGC
CAGCAACGAATATATCTATCTGGCTTCTCTCTTTGCGTTGGGTTTCCTGGTCTTTGGCCCGCAATTGTTGATTGGTGTGG
CTGCTGTTGGCTTTGTACCTAAAAAAGCGATTGGCGCTGCCGATGGTATTAAAGGCACCTTTGCTTACCTGATTGGTGAC
AGCTTTGCCAAGTTAGGTCTGGGAATGATTGCCGATGGGACGCCGGTATTCGGCCTTACCGGCTGGGCAGGCACATTCGC
CGCACTTGATATCGCCGCGATTGGTTGTATCTGCCTGATGGCGATAGTGGCGGTGATGGAAGAACGCAAAATCCGCCGCG
AGAAAAAAATTCAGCAGTTGACAGTGGCATAA

Upstream 100 bases:

>100_bases
AGAAATTTTCCAGGTTTTGCCTGGACGCTATCTCAGGCCTGATTTGCTGCTGATTTTTACAATGCATGCCTCACGCAGGT
ATTCATTTCAGGAGTAACCC

Downstream 100 bases:

>100_bases
ACGTAACTGGTGACTTTTGCCCGGCATGACGCCGGGCTTTTTTATTATTCCGTGACTTCCAGCGTAGTGAAGGCAAACTT
CTCGCCATCAAATAGCCCCT

Product: sugar phosphate antiporter

Products: Proton [Cytoplasm]; glucose-6-phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYLIRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGV
GKTLVSYYADGKNTKQFLPFMLILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPESYGLGKAEELFGEEISEEDKET
ESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRIGIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSD
LANGRRGLVACIALALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKGTFAYLIGD
SFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERKIRREKKIQQLTVA

Sequences:

>Translated_463_residues
MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYLIRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGV
GKTLVSYYADGKNTKQFLPFMLILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPESYGLGKAEELFGEEISEEDKET
ESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRIGIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSD
LANGRRGLVACIALALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKGTFAYLIGD
SFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERKIRREKKIQQLTVA
>Mature_463_residues
MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYLIRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGV
GKTLVSYYADGKNTKQFLPFMLILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPESYGLGKAEELFGEEISEEDKET
ESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRIGIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSD
LANGRRGLVACIALALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKGTFAYLIGD
SFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERKIRREKKIQQLTVA

Specific function: Transport protein for sugar phosphate uptake

COG id: COG2271

COG function: function code G; Sugar phosphate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Organophosphate:Pi antiporter (OPA) (TC 2.A.1.4) family

Homologues:

Organism=Homo sapiens, GI256219598, Length=436, Percent_Identity=23.6238532110092, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI4503847, Length=436, Percent_Identity=23.6238532110092, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI256219483, Length=436, Percent_Identity=23.6238532110092, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI2367259, Length=463, Percent_Identity=100, Blast_Score=933, Evalue=0.0,
Organism=Escherichia coli, GI1788573, Length=457, Percent_Identity=31.7286652078775, Blast_Score=218, Evalue=5e-58,
Organism=Escherichia coli, GI87082310, Length=463, Percent_Identity=33.0453563714903, Blast_Score=192, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI17542316, Length=483, Percent_Identity=22.5672877846791, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24656866, Length=496, Percent_Identity=21.3709677419355, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI45550478, Length=496, Percent_Identity=21.3709677419355, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24656861, Length=493, Percent_Identity=21.501014198783, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UHPT_ECO57 (P0AGC1)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C91204
- RefSeq:   NP_290301.1
- RefSeq:   NP_312630.1
- ProteinModelPortal:   P0AGC1
- EnsemblBacteria:   EBESCT00000028520
- EnsemblBacteria:   EBESCT00000056367
- GeneID:   915432
- GeneID:   960810
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5156
- KEGG:   ecs:ECs4603
- GeneTree:   EBGT00050000008831
- HOGENOM:   HBG559184
- OMA:   YSTITRW
- ProtClustDB:   PRK09556
- BioCyc:   ECOL83334:ECS4603-MONOMER
- InterPro:   IPR000849
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR021159
- TIGRFAMs:   TIGR00881

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 50607; Mature: 50607

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS00942 GLPT; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1d1028e4)-; HASH(0x1d866b00)-; HASH(0x1dd6be8c)-; HASH(0x1dc26244)-; HASH(0x1d84a700)-; HASH(0x1dd5b610)-; HASH(0x1ce06680)-; HASH(0x1dd6bbec)-; HASH(0x1d55f36c)-; HASH(0x1d9be33c)-; HASH(0x1d13dfe0)-; HASH(0x1d5b961c)-;

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
>Mature Secondary Structure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CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; glucose-6-phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + glucose-6-phosphate [Periplasm] = Proton [Cytoplasm] + glucose-6-phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796