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The map label for this gene is livH

Identifier: 218697140

GI number: 218697140

Start: 3933115

End: 3934041

Strand: Reverse

Name: livH

Synonym: EC55989_3865

Alternate gene names: 218697140

Gene position: 3934041-3933115 (Counterclockwise)

Preceding gene: 218697141

Following gene: 218697139

Centisome position: 76.32

GC content: 55.99

Gene sequence:

>927_bases
ATGTCTGAGCAGTTTTTGTATTTCTTGCAGCAGATGTTTAACGGCGTCACGCTGGGCAGTACCTACGCGCTGATAGCCAT
CGGCTACACCATGGTTTACGGCATTATCGGCATGATCAACTTCGCCCACGGCGAGGTTTATATGATTGGCAGCTACGTCT
CATTTATGATCATCGCCGCGCTGATGATGATGGGCATTGATACCGGCTGGCTGCTGGTGGCGGCGGGATTCGTCGGCGCA
ATCGTCATTGCCAGCGCCTACGGCTGGAGTATCGAACGGGTGGCTTACCGCCCGGTGCGTAATTCTAAGCGCCTGATTGC
GCTTATTTCCGCAATCGGTATGTCCATCTTCCTGCAAAACTACGTCAGCCTGACCGAAGGTTCGCGCGATGTGGCGCTGC
CCAGCCTGTTTAACGGTCAGTGGGTGGTGGGCCATAGCGAAAACTTCTCTGCCTCTATTACCACCATGCAGGCGGTGATC
TGGATTGTCACCTTCCTCGCCATGCTGGCGCTGACGATTTTCATTCGCTATTCCCGTATGGGGCGCGCCTGTCGTGCCTG
CGCGGAAGATCTGAAAATGGCGAGTCTGCTTGGCATTAACACCGACCGGGTGATTGCGCTGACCTTTGTGATTGGCGCGG
CGATGGCGGCGGTGGCGGGTGTGCTGCTCGGTCAGTTCTACGGCGTAATTAACCCCTACATCGGCTTTATGGCCGGGATG
AAAGCCTTTACCGCTGCGGTGCTCGGTGGGATTGGCAGCATTCCAGGGGCGATGATTGGTGGGCTGATCCTGGGGATTGC
GGAGGCGCTCTCTTCTGCCTATCTGAGTACGGAATATAAGGATGTGGTTTCATTTGCCCTGCTGATTCTGGTGCTGCTGG
TGATGCCGACCGGTATTCTGGGTCGCCCGGAGGTAGAGAAAGTATGA

Upstream 100 bases:

>100_bases
ATTTTGGTGTCTTCCAGTGGCACGCCGACGGTTCATCCACGGCAGCCAAGTGATCATCCCACCGCCCGTAAAATGCGGGC
GGGTTTAGAAAGGTTACCTT

Downstream 100 bases:

>100_bases
AACCGATGCATATTGCAATGGCGCTGCTCTCTGCCGCGATGTTTTTTGTCCTGGCGGGCGTCTTTATGGGCGTGCAACTG
GAGCTGGATGGCACCAAACT

Product: branched-chain amino acid transporter permease subunit LivH

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein H

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHGEVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGA
IVIASAYGWSIERVAYRPVRNSKRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAVAGVLLGQFYGVINPYIGFMAGM
KAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLSTEYKDVVSFALLILVLLVMPTGILGRPEVEKV

Sequences:

>Translated_308_residues
MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHGEVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGA
IVIASAYGWSIERVAYRPVRNSKRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAVAGVLLGQFYGVINPYIGFMAGM
KAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLSTEYKDVVSFALLILVLLVMPTGILGRPEVEKV
>Mature_307_residues
SEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHGEVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAI
VIASAYGWSIERVAYRPVRNSKRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVIW
IVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAVAGVLLGQFYGVINPYIGFMAGMK
AFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLSTEYKDVVSFALLILVLLVMPTGILGRPEVEKV

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily

Homologues:

Organism=Escherichia coli, GI1789866, Length=308, Percent_Identity=100, Blast_Score=608, Evalue=1e-175,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIVH_ECO57 (P0AEX8)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   H86012
- PIR:   H91166
- RefSeq:   NP_290003.1
- RefSeq:   NP_312331.1
- ProteinModelPortal:   P0AEX8
- EnsemblBacteria:   EBESCT00000026019
- EnsemblBacteria:   EBESCT00000057276
- GeneID:   915841
- GeneID:   961373
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4827
- KEGG:   ecs:ECs4304
- GeneTree:   EBGT00050000010183
- HOGENOM:   HBG728273
- OMA:   AYFTIAY
- ProtClustDB:   PRK10740
- BioCyc:   ECOL83334:ECS4304-MONOMER
- InterPro:   IPR001851

Pfam domain/function: PF02653 BPD_transp_2

EC number: NA

Molecular weight: Translated: 32983; Mature: 32852

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1d4ca76c)-; HASH(0x1d915280)-; HASH(0x1d5ed548)-; HASH(0x1d5ed5d8)-; HASH(0x1d5ed77c)-; HASH(0x1ce2b01c)-; HASH(0x1d93e510)-; HASH(0x1d93e6fc)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHGEVYMIGSYVSFMIIAA
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
LMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNSKRLIALISAIGMSIFLQN
HHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
YVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVIWIVTFLAMLALTIFIRYSRM
HHHHHCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAVAGVLLGQFYGVINPYIGFMAGM
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLSTEYKDVVSFALLILVLLVMPTGIL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GRPEVEKV
CCCCCCCH
>Mature Secondary Structure 
SEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHGEVYMIGSYVSFMIIAA
CHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH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CCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796