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The map label for this gene is glpR

Identifier: 218697105

GI number: 218697105

Start: 3895093

End: 3895851

Strand: Reverse

Name: glpR

Synonym: EC55989_3830

Alternate gene names: 218697105

Gene position: 3895851-3895093 (Counterclockwise)

Preceding gene: 218697106

Following gene: 218697103

Centisome position: 75.58

GC content: 53.89

Gene sequence:

>759_bases
ATGAAACAAACACAACGTCACAACGGTATTATCGAACTGGTTAAACAGCAGGGTTATGTCAGTACTGAAGAGCTGGTAGA
GCATTTCTCCGTCAGCCCGCAGACTATTCGCCGCGACCTCAATGAGCTGGCGGAGCAAAACCTGATCCTGCGTCACCATG
GCGGTGCGGCGCTGCCTTCCAGTTCGGTTAACACGCCGTGGCACGATCGCAAAGCCACCCAGACCGAAGAAAAAGAGAGG
ATTGCCCGCAAAGTGGCGGAGCAAATCCCCAACGGCTCGACACTGTTTATCGATATCGGCACCACGCCGGAAGCGGTGGC
GCACGCGCTGCTTAATCACAGCAATTTGCGTATTGTCACCAACAATCTCAATGTTGCTAACACGTTGATGGTAAAAGAAG
ATTTCCGCATTATTCTCGCCGGTGGCGAATTACGCAGCCGCGATGGCGGGATCATTGGCGAAGCGACGCTCGATTTTATC
TCCCAGTTCCGCCTTGATTTCGGCATTCTGGGGATAAGCGGCATCGATAGCGACGGCTCGCTGCTGGAGTTCGATTACCA
CGAAGTTCGCACCAAGCGCGCCATTATTGAGAACTCGCGCCACGTTATGCTGGTTGTCGATCACTCGAAATTTGGCCGTA
ACGCGATGGTCAATATGGGCAGCATCAGCATGGTAGATGCCGTCTACACCGACGCCCCGCCGCCAGTAAGCGTAATGCAG
GTGCTGACGGACCATCATATTCAACTGGAGCTGTGCTGA

Upstream 100 bases:

>100_bases
GCGAACGGAGCACACATCGCCGGGTTAGCCGTGGGTTTAGCGATGGCTTTTGTTGATTCGCTCAATGCGCGAAAACGAAA
ATAATTCCAGGGATTTATAA

Downstream 100 bases:

>100_bases
TCCTGCACGGCTTCCCACGTCAGACCAAAACGCGCCAGGTATTTGCGTAGCCGATCCGCGTCATTGACGCTGGCTTTGCC
CTGGCGCGAAACATCAAAAA

Product: DNA-binding transcriptional repressor GlpR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELAEQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKER
IARKVAEQIPNGSTLFIDIGTTPEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVNMGSISMVDAVYTDAPPPVSVMQ
VLTDHHIQLELC

Sequences:

>Translated_252_residues
MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELAEQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKER
IARKVAEQIPNGSTLFIDIGTTPEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVNMGSISMVDAVYTDAPPPVSVMQ
VLTDHHIQLELC
>Mature_252_residues
MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELAEQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKER
IARKVAEQIPNGSTLFIDIGTTPEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVNMGSISMVDAVYTDAPPPVSVMQ
VLTDHHIQLELC

Specific function: Repressor of the glycerol-3-phosphate regulon

COG id: COG1349

COG function: function code KG; Transcriptional regulators of sugar metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH deoR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1789829, Length=252, Percent_Identity=100, Blast_Score=518, Evalue=1e-148,
Organism=Escherichia coli, GI87082344, Length=246, Percent_Identity=44.7154471544715, Blast_Score=229, Evalue=1e-61,
Organism=Escherichia coli, GI1788069, Length=251, Percent_Identity=29.0836653386454, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789059, Length=251, Percent_Identity=29.8804780876494, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1789519, Length=251, Percent_Identity=28.2868525896414, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI226510968, Length=250, Percent_Identity=30.4, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1789170, Length=232, Percent_Identity=28.448275862069, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1787063, Length=253, Percent_Identity=26.8774703557312, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790753, Length=253, Percent_Identity=24.1106719367589, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1787540, Length=229, Percent_Identity=27.9475982532751, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790635, Length=247, Percent_Identity=24.6963562753036, Blast_Score=78, Evalue=6e-16,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GLPR_ECOLI (P0ACL0)

Other databases:

- EMBL:   M96795
- EMBL:   X07520
- EMBL:   U18997
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   B65138
- RefSeq:   AP_004368.1
- RefSeq:   NP_417881.1
- ProteinModelPortal:   P0ACL0
- SMR:   P0ACL0
- IntAct:   P0ACL0
- STRING:   P0ACL0
- SWISS-2DPAGE:   P0ACL0
- EnsemblBacteria:   EBESCT00000004882
- EnsemblBacteria:   EBESCT00000004883
- EnsemblBacteria:   EBESCT00000004884
- EnsemblBacteria:   EBESCT00000004885
- EnsemblBacteria:   EBESCT00000015950
- GeneID:   947926
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3386
- KEGG:   eco:b3423
- EchoBASE:   EB0395
- EcoGene:   EG10400
- eggNOG:   COG1349
- GeneTree:   EBGT00070000031699
- HOGENOM:   HBG694151
- OMA:   ATQTAEK
- ProtClustDB:   PRK10906
- BioCyc:   EcoCyc:PD03576
- Genevestigator:   P0ACL0
- GO:   GO:0005622
- InterPro:   IPR014036
- InterPro:   IPR001034
- InterPro:   IPR018356
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00037
- SMART:   SM00420

Pfam domain/function: PF00455 DeoR; PF08220 HTH_DeoR

EC number: NA

Molecular weight: Translated: 28048; Mature: 28048

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00894 HTH_DEOR_1; PS51000 HTH_DEOR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELAEQNLILRHHGGAALPS
CCCCCHHCHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCEEEEECCCCCCCC
SSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTTPEAVAHALLNHSNLRIVT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCEEEEE
NNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFISQFRLDFGILGISGIDSDGS
CCCCEEEEEEEECCCEEEEECCCEECCCCCEEHHHHHHHHHHHHHHHEEEEEECCCCCCC
LLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVNMGSISMVDAVYTDAPPPVSVMQ
EEEEHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEECCCHHHHHHHHCCCCCCHHHHH
VLTDHHIQLELC
HHHCCCEEEEEC
>Mature Secondary Structure
MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELAEQNLILRHHGGAALPS
CCCCCHHCHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCEEEEECCCCCCCC
SSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTTPEAVAHALLNHSNLRIVT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCEEEEE
NNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFISQFRLDFGILGISGIDSDGS
CCCCEEEEEEEECCCEEEEECCCEECCCCCEEHHHHHHHHHHHHHHHEEEEEECCCCCCC
LLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVNMGSISMVDAVYTDAPPPVSVMQ
EEEEHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEECCCHHHHHHHHCCCCCCHHHHH
VLTDHHIQLELC
HHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8955387; 3045764; 9278503