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The map label for this gene is parE

Identifier: 218696736

GI number: 218696736

Start: 3523594

End: 3525486

Strand: Reverse

Name: parE

Synonym: EC55989_3446

Alternate gene names: 218696736

Gene position: 3525486-3523594 (Counterclockwise)

Preceding gene: 218696737

Following gene: 218696733

Centisome position: 68.39

GC content: 53.94

Gene sequence:

>1893_bases
ATGACGCAAACTTATAACGCTGATGCCATTGAGGTACTCACCGGGCTTGAGCCGGTTCGCCGCCGTCCGGGGATGTATAC
CGATACCACTCGCCCTAACCATTTGGGGCAAGAAGTTATTGATAACAGTGTCGATGAAGCACTGGCGGGTCACGCAAAAC
GCGTGGATGTAATCTTACATGCTGACCAGTCGTTAGAAGTTATTGACGATGGGCGCGGGATGCCGGTGGATATTCACCCG
GAAGAGGGTGTACCGGCGGTTGAACTGATTCTTTGCCGTCTGCATGCAGGCGGTAAATTCTCTAACAAAAATTACCAGTT
CTCTGGCGGCCTGCATGGCGTGGGGATTTCGGTGGTTAACGCCCTGTCGAAGCGCGTAGAAGTTAACGTGCGCCGCGATG
GTCAGGTTTATAACATCGCCTTTGAAAATGGCGAAAAGGTGCAGGATTTACAGGTTGTCGGCACTTGCGGTAAACGCAAT
ACCGGTACCAGCGTGCACTTCTGGCCGGATGAAACCTTCTTTGACAGCCCGCGTTTTTCTGTTTCACGCCTGACGCATGT
GCTGAAGGCCAAAGCAGTACTGTGCCCCGGCGTTGAGATTACCTTTAAAGATGAGATCAACAACACCGAACAGCGCTGGT
GCTATCAGGACGGTCTGAATGATTACCTGGCGGAAGCGGTAAACGGTTTACCGACGCTGCCAGAAAAACCGTTTATCGGT
AATTTCGCTGGCGATACTGAAGCTGTGGACTGGGCGCTACTGTGGCTGCCGGAAGGCGGTGAACTGCTGACCGAAAGCTA
CGTCAACCTTATCCCAACGATGCAGGGCGGTACCCATGTTAATGGTCTGCGTCAGGGCCTGTTGGACGCGATGCGTGAGT
TCTGTGAATACCGCAACATTCTGCCGCGCGGCGTGAAGCTGTCGGCGGAAGATATCTGGGATCGCTGCGCCTATGTTCTG
TCGGTGAAAATGCAGGATCCGCAGTTTGCCGGGCAGACCAAAGAGCGTCTCTCTTCGCGTCAATGCGCGGCATTCGTTTC
AGGCGTGGTGAAAGATGCCTTTATCCTGTGGCTGAACCAGAACGTTCAGGCGGCGGAGCTGCTGGCGGAGATGGCGATTT
CCAGCGCCCAGCGCCGTATGCGTGCGGCTAAAAAAGTGGTGCGCAAAAAGCTGACCAGCGGCCCAGCACTGCCTGGCAAA
CTGGCTGACTGTACCGCGCAGGATCTTAACCGTACCGAACTGTTCCTTGTGGAAGGTGACTCCGCAGGCGGATCTGCCAA
GCAGGCGCGCGATCGCGAATATCAGGCGATCATGCCACTGAAAGGTAAGATCCTTAACACCTGGGAAGTTTCTTCCGACG
AAGTGCTGGCCTCGCAGGAAGTGCATGATATTTCGGTAGCGATCGGTATCGATCCTGACAGCGACGATCTGAGCCAGCTT
CGTTATGGCAAAATCTGTATCCTCGCGGATGCGGACTCTGATGGTCTGCACATTGCCACGCTGCTCTGCGCTTTGTTCGT
AAAACACTTCCGCGCGTTGGTGAAACACGGTCACGTTTACGTCGCACTGCCACCGCTCTACCGTATTGATCTCGGGAAAG
AGGTTTATTACGCGCTGACGGAAGAAGAGAAAGAGGGCGTACTTGAGCAATTAAAACGCAAGAAAGGCAAGCCGAACGTC
CAGCGTTTTAAAGGTCTGGGGGAAATGAACCCGATGCAATTGCGTGAAACCACGCTTGATCCGAACACTCGCCGTCTGGT
GCAGTTGACTATCGATGATGAAGACGATCAGCGTACTGACGCGATGATGGATATGCTGCTGGCGAAGAAACGCTCGGAAG
ATCGCCGCAACTGGTTGCAAGAGAAAGGCGACATGGCGGAGATTGAGGTTTAA

Upstream 100 bases:

>100_bases
AACCACGCATTCACGGGCTTCGAAGATTATTTCAACCCGATCGTCGATTTTCTTGGTCTGCACCATCTCTGACGATCAAC
TCGAATTGCTAACTTAAACC

Downstream 100 bases:

>100_bases
AGGAAAGAACATTGCCCGGTCAGGATGGCCGGGCAAACAAGGCAGGTTTAAATCCCAGGTTGCAGGATGCGGATATTCAT
CTCCAGCACGTCGCCTTTTA

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B

Number of amino acids: Translated: 630; Mature: 629

Protein sequence:

>630_residues
MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHP
EEGVPAVELILCRLHAGGKFSNKNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDGLNDYLAEAVNGLPTLPEKPFIG
NFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQGGTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVL
SVKMQDPQFAGQTKERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLTSGPALPGK
LADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEVSSDEVLASQEVHDISVAIGIDPDSDDLSQL
RYGKICILADADSDGLHIATLLCALFVKHFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNV
QRFKGLGEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGDMAEIEV

Sequences:

>Translated_630_residues
MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHP
EEGVPAVELILCRLHAGGKFSNKNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDGLNDYLAEAVNGLPTLPEKPFIG
NFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQGGTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVL
SVKMQDPQFAGQTKERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLTSGPALPGK
LADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEVSSDEVLASQEVHDISVAIGIDPDSDDLSQL
RYGKICILADADSDGLHIATLLCALFVKHFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNV
QRFKGLGEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGDMAEIEV
>Mature_629_residues
TQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPE
EGVPAVELILCRLHAGGKFSNKNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRNT
GTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDGLNDYLAEAVNGLPTLPEKPFIGN
FAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQGGTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLS
VKMQDPQFAGQTKERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLTSGPALPGKL
ADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEVSSDEVLASQEVHDISVAIGIDPDSDDLSQLR
YGKICILADADSDGLHIATLLCALFVKHFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQ
RFKGLGEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGDMAEIEV

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family

Homologues:

Organism=Homo sapiens, GI19913408, Length=577, Percent_Identity=25.1299826689775, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI19913406, Length=582, Percent_Identity=24.0549828178694, Blast_Score=121, Evalue=2e-27,
Organism=Escherichia coli, GI1789408, Length=630, Percent_Identity=100, Blast_Score=1306, Evalue=0.0,
Organism=Escherichia coli, GI48994957, Length=555, Percent_Identity=40.7207207207207, Blast_Score=382, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI212645845, Length=606, Percent_Identity=24.0924092409241, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17535065, Length=597, Percent_Identity=24.4556113902848, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI212645657, Length=228, Percent_Identity=26.7543859649123, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=683, Percent_Identity=23.7188872620791, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI17136538, Length=582, Percent_Identity=22.8522336769759, Blast_Score=115, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PARE_ECOLI (P20083)

Other databases:

- EMBL:   M58409
- EMBL:   U28377
- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   L22026
- PIR:   D65090
- RefSeq:   AP_003580.1
- RefSeq:   NP_417502.1
- PDB:   1S14
- PDB:   1S16
- PDB:   3FV5
- PDBsum:   1S14
- PDBsum:   1S16
- PDBsum:   3FV5
- ProteinModelPortal:   P20083
- DIP:   DIP-10441N
- MINT:   MINT-1223307
- STRING:   P20083
- EnsemblBacteria:   EBESCT00000001166
- EnsemblBacteria:   EBESCT00000016957
- GeneID:   947501
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2998
- KEGG:   eco:b3030
- EchoBASE:   EB0681
- EcoGene:   EG10687
- eggNOG:   COG0187
- GeneTree:   EBGT00050000009515
- HOGENOM:   HBG304889
- OMA:   EKGDMAD
- ProtClustDB:   PRK05559
- BioCyc:   EcoCyc:EG10687-MONOMER
- Genevestigator:   P20083
- GO:   GO:0005694
- GO:   GO:0005737
- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737
- Gene3D:   G3DSA:3.30.565.10
- Gene3D:   G3DSA:3.30.230.10
- Gene3D:   G3DSA:3.40.50.670
- PRINTS:   PR00418
- SMART:   SM00387
- SMART:   SM00433
- TIGRFAMs:   TIGR01055

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; SSF55874 ATP_bd_ATPase; SSF54211 Ribosomal_S5_D2-typ_fold; SSF56719 Topo_IIA_cen

EC number: 5.99.1.-

Molecular weight: Translated: 70245; Mature: 70114

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGHAKRVDVILH
CCCCCCCCHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEE
ADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSNKNYQFSGGLHGVGISVVN
CCCCCEEEECCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHH
ALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRNTGTSVHFWPDETFFDSPRFS
HHHHHEEEEEECCCEEEEEEECCCCCEEHEEEEECCCCCCCCCEEEECCCCCCCCCCCHH
VSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDGLNDYLAEAVNGLPTLPEKPFIG
HHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCC
NFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQGGTHVNGLRQGLLDAMREFCEYRNI
CCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
LPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTKERLSSRQCAAFVSGVVKDAFILWLNQ
CCCCCEECHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECC
NVQAAELLAEMAISSAQRRMRAAKKVVRKKLTSGPALPGKLADCTAQDLNRTELFLVEGD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHCCCCEEEEEEECC
SAGGSAKQARDREYQAIMPLKGKILNTWEVSSDEVLASQEVHDISVAIGIDPDSDDLSQL
CCCCCHHHHHCCCCEEECCCCCCEEEEEECCCCCHHHCCCCEEEEEEEECCCCCHHHHHH
RYGKICILADADSDGLHIATLLCALFVKHFRALVKHGHVYVALPPLYRIDLGKEVYYALT
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCCCEEEEEC
EEEKEGVLEQLKRKKGKPNVQRFKGLGEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTD
CHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHCCCCCCCEEEEEEEECCCCCHHHH
AMMDMLLAKKRSEDRRNWLQEKGDMAEIEV
HHHHHHHHHHCCHHHHHHHHHCCCEEECCC
>Mature Secondary Structure 
TQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGHAKRVDVILH
CCCCCCCHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEE
ADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSNKNYQFSGGLHGVGISVVN
CCCCCEEEECCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHH
ALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRNTGTSVHFWPDETFFDSPRFS
HHHHHEEEEEECCCEEEEEEECCCCCEEHEEEEECCCCCCCCCEEEECCCCCCCCCCCHH
VSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDGLNDYLAEAVNGLPTLPEKPFIG
HHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCC
NFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQGGTHVNGLRQGLLDAMREFCEYRNI
CCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
LPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTKERLSSRQCAAFVSGVVKDAFILWLNQ
CCCCCEECHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECC
NVQAAELLAEMAISSAQRRMRAAKKVVRKKLTSGPALPGKLADCTAQDLNRTELFLVEGD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHCCCCEEEEEEECC
SAGGSAKQARDREYQAIMPLKGKILNTWEVSSDEVLASQEVHDISVAIGIDPDSDDLSQL
CCCCCHHHHHCCCCEEECCCCCCEEEEEECCCCCHHHCCCCEEEEEEEECCCCCHHHHHH
RYGKICILADADSDGLHIATLLCALFVKHFRALVKHGHVYVALPPLYRIDLGKEVYYALT
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCCCEEEEEC
EEEKEGVLEQLKRKKGKPNVQRFKGLGEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTD
CHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHCCCCCCCEEEEEEEECCCCCHHHH
AMMDMLLAKKRSEDRRNWLQEKGDMAEIEV
HHHHHHHHHHCCHHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2170028; 8388096; 9278503; 8227000; 8980775