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The map label for this gene is oprF [H]

Identifier: 218696635

GI number: 218696635

Start: 3423337

End: 3425163

Strand: Reverse

Name: oprF [H]

Synonym: EC55989_3336

Alternate gene names: 218696635

Gene position: 3425163-3423337 (Counterclockwise)

Preceding gene: 218696636

Following gene: 218696634

Centisome position: 66.45

GC content: 57.2

Gene sequence:

>1827_bases
ATGGGGGGCGGGTATTGTGACGCTGGCTGGTCTCTGGTGTGTGCTTTCTTCTGTGCTTGCAGACCAGGTGGCACGGCTTA
CCGGGCAGGGATAACCATGCACGTACGTTCCCGTCTCTGGCTGACAATACTGAGTCTGTTCCTGACACTGTGGCTGATTT
CGGGATTCCTGCCGCTGTCTGCAGTAAACCAGATAATACTGACCCTGGTTTCAGTTGTGGCGGCGGGGCTTGTGCTGTGG
TACCAGTGGCGTTGTCTGTCTTCCCGTCGTGATATCCCGGAGAAGGCCGGCGATGACGGTCTGCCGCCTGAGTATTTTCA
GGGACATGTGCTGCTTGTTGCCGGAAACTCAGATGCGTGGTTCGAACCCGGTCAGGCATACCGGGAAACAGCATCGGGAT
GGTATCTTCGTGTGGATACGCCGGAGCAGATGAGAATACTGGCAGAACGGTTGTCGTACAGCCGTCCGGCCCTGTGTGGC
CAGGTCGTGGTCATGCTGGGACTTTTACCGGAGCAGCATACCTCAGATGAAGCTTTCCTGTCGTGGTTGCGGAACTGGCA
GCGTGCAGTCATGCAGTGCCGGCAGTGGCTGAATACCACGCCACCGGTGCTGGTTACATTGATGGTTTCAGAGCCGTCAC
CACAAAACACATCCTTAAATAAGCACTCTACACACTGGTTTACACTGACACCGGACCTGCCGGGAATGCATATACGCCTG
ACGGAAGCCGGAGCAATTCCGGCAGAGGAATGGCTGCAGGCGTGTGATGCAGCGTCCAGGCTCCGGTGCGTCAGTGAACT
GTTATGGCTGAATGCCATGTTGTCCTGGTACAACCGGGTTACTGATGCCCTGACAGCTATTTGCCGGCAGGAGCACTTTT
ATTTCCGGCCGGTTGCCGTTGGATTCTGTCTGACGTCGGTTGCGGCCCGCCCCGGTAATCTCTGGCAACAACAACTGACG
TCCCTCACTGCATTGCCACCGCCTGCCGGCGTGTCATCACAGGTATTGCCGTTGCCGGATATTCTGCTGTCCGGTATTCC
CCGTCATCGTGGACTCAGTCACTGGCACAGACTTTGTCGCCAGACGGGGGCGATTGTCGGTGTTTTTCTTCTGCTGGCTA
TGCTGGCGTCTTTTATGAATAACCAGCGGCTGGTTCGCAGCGTGGGGGAACACCTGACGGCCTGGCACCGGATGCGCGGA
TATTCATCTGAATCGAAAACAACAGCACTGCAACGTCTTCAGGCTGACAGCCAGTTACTGAACGACTGGCAGCAACGTGG
TGTGCCACAGCGTTACGGACTGGGATTGTATCAGGGAATGTGGCTGATACCGCCGGTTGAGCGTGCGACTGGTGAGTGGG
TTGCACCACGGCCTCCCCGTCCGGTGATACAGAAAGTTACTCAGACGCCGAAAACGGTTCGCCTTAACAGCCTGGCACTG
TTTGATGCCGGTAAATGGACGCTGAAGCCGGGCGCCACAAAATGGCTGGTGAATGCCCTGGTGGATATTAAGGCAAAAGC
AGGCTGGCTGATTGTGGTCAGTGGTCACACGGATAATACCGGTGACCCGCTGCGCAACCAGGCCTTGTCCCTGAAGCGTG
CAGAGGCCGTTCGCGACTGGATGCGGGATACCGGCGATATTCCCCAAAGCTGTTTTGCCGTACAGGGATACGGTGAAAGC
CGCCCTGTGGCCCCGAATGACACGGCGGAAGGCCGTGCGCGTAACCGCCGTGTTGAAATCAGTCTGGTACCGCAGGCCGA
TGCCTGTCGTCTGCCGGACGTTTCCCCTGCGCCGGAAGAGGGCGCATCAGAAAACATAACGGAGTAA

Upstream 100 bases:

>100_bases
CACTGACAGCGCGTGTTCCTGCATTTACTTTCGCACAGGATGCGCCGGTGGTGGTGCGTGCACCGGGTTACCGTGCTGGC
AGAACGATGTACTGGTTGTC

Downstream 100 bases:

>100_bases
TTTTTATGGCAATTCCAGTTTATCTGTGGCTGAAAGATGATGGCGGTGCAGATATCAAAGGGTCCGTGGATGTTCAGGAC
CGTGAGGGCAGCATTGAGGT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 608; Mature: 607

Protein sequence:

>608_residues
MGGGYCDAGWSLVCAFFCACRPGGTAYRAGITMHVRSRLWLTILSLFLTLWLISGFLPLSAVNQIILTLVSVVAAGLVLW
YQWRCLSSRRDIPEKAGDDGLPPEYFQGHVLLVAGNSDAWFEPGQAYRETASGWYLRVDTPEQMRILAERLSYSRPALCG
QVVVMLGLLPEQHTSDEAFLSWLRNWQRAVMQCRQWLNTTPPVLVTLMVSEPSPQNTSLNKHSTHWFTLTPDLPGMHIRL
TEAGAIPAEEWLQACDAASRLRCVSELLWLNAMLSWYNRVTDALTAICRQEHFYFRPVAVGFCLTSVAARPGNLWQQQLT
SLTALPPPAGVSSQVLPLPDILLSGIPRHRGLSHWHRLCRQTGAIVGVFLLLAMLASFMNNQRLVRSVGEHLTAWHRMRG
YSSESKTTALQRLQADSQLLNDWQQRGVPQRYGLGLYQGMWLIPPVERATGEWVAPRPPRPVIQKVTQTPKTVRLNSLAL
FDAGKWTLKPGATKWLVNALVDIKAKAGWLIVVSGHTDNTGDPLRNQALSLKRAEAVRDWMRDTGDIPQSCFAVQGYGES
RPVAPNDTAEGRARNRRVEISLVPQADACRLPDVSPAPEEGASENITE

Sequences:

>Translated_608_residues
MGGGYCDAGWSLVCAFFCACRPGGTAYRAGITMHVRSRLWLTILSLFLTLWLISGFLPLSAVNQIILTLVSVVAAGLVLW
YQWRCLSSRRDIPEKAGDDGLPPEYFQGHVLLVAGNSDAWFEPGQAYRETASGWYLRVDTPEQMRILAERLSYSRPALCG
QVVVMLGLLPEQHTSDEAFLSWLRNWQRAVMQCRQWLNTTPPVLVTLMVSEPSPQNTSLNKHSTHWFTLTPDLPGMHIRL
TEAGAIPAEEWLQACDAASRLRCVSELLWLNAMLSWYNRVTDALTAICRQEHFYFRPVAVGFCLTSVAARPGNLWQQQLT
SLTALPPPAGVSSQVLPLPDILLSGIPRHRGLSHWHRLCRQTGAIVGVFLLLAMLASFMNNQRLVRSVGEHLTAWHRMRG
YSSESKTTALQRLQADSQLLNDWQQRGVPQRYGLGLYQGMWLIPPVERATGEWVAPRPPRPVIQKVTQTPKTVRLNSLAL
FDAGKWTLKPGATKWLVNALVDIKAKAGWLIVVSGHTDNTGDPLRNQALSLKRAEAVRDWMRDTGDIPQSCFAVQGYGES
RPVAPNDTAEGRARNRRVEISLVPQADACRLPDVSPAPEEGASENITE
>Mature_607_residues
GGGYCDAGWSLVCAFFCACRPGGTAYRAGITMHVRSRLWLTILSLFLTLWLISGFLPLSAVNQIILTLVSVVAAGLVLWY
QWRCLSSRRDIPEKAGDDGLPPEYFQGHVLLVAGNSDAWFEPGQAYRETASGWYLRVDTPEQMRILAERLSYSRPALCGQ
VVVMLGLLPEQHTSDEAFLSWLRNWQRAVMQCRQWLNTTPPVLVTLMVSEPSPQNTSLNKHSTHWFTLTPDLPGMHIRLT
EAGAIPAEEWLQACDAASRLRCVSELLWLNAMLSWYNRVTDALTAICRQEHFYFRPVAVGFCLTSVAARPGNLWQQQLTS
LTALPPPAGVSSQVLPLPDILLSGIPRHRGLSHWHRLCRQTGAIVGVFLLLAMLASFMNNQRLVRSVGEHLTAWHRMRGY
SSESKTTALQRLQADSQLLNDWQQRGVPQRYGLGLYQGMWLIPPVERATGEWVAPRPPRPVIQKVTQTPKTVRLNSLALF
DAGKWTLKPGATKWLVNALVDIKAKAGWLIVVSGHTDNTGDPLRNQALSLKRAEAVRDWMRDTGDIPQSCFAVQGYGESR
PVAPNDTAEGRARNRRVEISLVPQADACRLPDVSPAPEEGASENITE

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI1788957, Length=109, Percent_Identity=35.7798165137615, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI48994946, Length=121, Percent_Identity=37.1900826446281, Blast_Score=70, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 67736; Mature: 67605

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGGYCDAGWSLVCAFFCACRPGGTAYRAGITMHVRSRLWLTILSLFLTLWLISGFLPLS
CCCCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
AVNQIILTLVSVVAAGLVLWYQWRCLSSRRDIPEKAGDDGLPPEYFQGHVLLVAGNSDAW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHCCCEEEEECCCCCC
FEPGQAYRETASGWYLRVDTPEQMRILAERLSYSRPALCGQVVVMLGLLPEQHTSDEAFL
CCCCHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHH
SWLRNWQRAVMQCRQWLNTTPPVLVTLMVSEPSPQNTSLNKHSTHWFTLTPDLPGMHIRL
HHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCEEEE
TEAGAIPAEEWLQACDAASRLRCVSELLWLNAMLSWYNRVTDALTAICRQEHFYFRPVAV
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHH
GFCLTSVAARPGNLWQQQLTSLTALPPPAGVSSQVLPLPDILLSGIPRHRGLSHWHRLCR
HHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHH
QTGAIVGVFLLLAMLASFMNNQRLVRSVGEHLTAWHRMRGYSSESKTTALQRLQADSQLL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
NDWQQRGVPQRYGLGLYQGMWLIPPVERATGEWVAPRPPRPVIQKVTQTPKTVRLNSLAL
HHHHHCCCCHHHCCHHHCCEECCCCHHHCCCCCCCCCCCHHHHHHHHCCCCEEEECEEEE
FDAGKWTLKPGATKWLVNALVDIKAKAGWLIVVSGHTDNTGDPLRNQALSLKRAEAVRDW
EECCCEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
MRDTGDIPQSCFAVQGYGESRPVAPNDTAEGRARNRRVEISLVPQADACRLPDVSPAPEE
HHHCCCCCHHHEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCC
GASENITE
CCCCCCCC
>Mature Secondary Structure 
GGGYCDAGWSLVCAFFCACRPGGTAYRAGITMHVRSRLWLTILSLFLTLWLISGFLPLS
CCCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
AVNQIILTLVSVVAAGLVLWYQWRCLSSRRDIPEKAGDDGLPPEYFQGHVLLVAGNSDAW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHCCCEEEEECCCCCC
FEPGQAYRETASGWYLRVDTPEQMRILAERLSYSRPALCGQVVVMLGLLPEQHTSDEAFL
CCCCHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHH
SWLRNWQRAVMQCRQWLNTTPPVLVTLMVSEPSPQNTSLNKHSTHWFTLTPDLPGMHIRL
HHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCEEEE
TEAGAIPAEEWLQACDAASRLRCVSELLWLNAMLSWYNRVTDALTAICRQEHFYFRPVAV
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHH
GFCLTSVAARPGNLWQQQLTSLTALPPPAGVSSQVLPLPDILLSGIPRHRGLSHWHRLCR
HHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHH
QTGAIVGVFLLLAMLASFMNNQRLVRSVGEHLTAWHRMRGYSSESKTTALQRLQADSQLL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
NDWQQRGVPQRYGLGLYQGMWLIPPVERATGEWVAPRPPRPVIQKVTQTPKTVRLNSLAL
HHHHHCCCCHHHCCHHHCCEECCCCHHHCCCCCCCCCCCHHHHHHHHCCCCEEEECEEEE
FDAGKWTLKPGATKWLVNALVDIKAKAGWLIVVSGHTDNTGDPLRNQALSLKRAEAVRDW
EECCCEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
MRDTGDIPQSCFAVQGYGESRPVAPNDTAEGRARNRRVEISLVPQADACRLPDVSPAPEE
HHHCCCCCHHHEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCC
GASENITE
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2447060; 10984043 [H]