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The map label for this gene is endA

Identifier: 218696543

GI number: 218696543

Start: 3319830

End: 3320537

Strand: Direct

Name: endA

Synonym: EC55989_3238

Alternate gene names: 218696543

Gene position: 3319830-3320537 (Clockwise)

Preceding gene: 218696542

Following gene: 218696544

Centisome position: 64.4

GC content: 54.8

Gene sequence:

>708_bases
ATGTACCGTTATTTGTCTATTGCTGCAGTGGTACTGAGCGCAGCATTTTCCGGCCCGGCGTTGGCCGAAGGTATCAATAG
TTTTTCTCAGGCGAAAGCCGCGGCGGTAAAAGTCCACGCTGACGCGCCCGGTACGTTTTATTGCGGATGTAAAATTAACT
GGCAGGGCAAAAAAGGCGTTGTTGATCTGCAATCGTGCGGCTATCAGGTGCGCAAAAATGAAAACCGCGCCAGCCGCGTA
GAGTGGGAACACGTCGTTCCCGCCTGGCAGTTCGGTCACCAGCGCCAGTGCTGGCAGGACGGTGGACGTAAAAACTGCGC
TAAAGATCCGGTCTATCGCAAGATGGAAAGCGATATGCATAACCTGCAGCCGTCAGTCGGTGAGGTGAATGGCGATCGCG
GCAACTTTATGTACAGCCAGTGGAATGGCGGCGAAGGCCAGTACGGTCAATGCGCCATGAAGGTCGATTTCAAAGAAAAA
GCTGCCGAACCACCAGCGCGTGCACGCGGTGCCATTGCGCGCACCTACTTCTATATGCGCGACCAATACAACCTGACACT
CTCTCGCCAGCAAACGCAGCTGTTCAACGCATGGAACAAGATGTATCCGGTTACCGACTGGGAGTGCGAGCGCGATGAAC
GCATCGCGAAGGTGCAAGGCAATCATAACCCGTATGTGCAACGCGCTTGCCAGGCGCGAAAGAGCTAA

Upstream 100 bases:

>100_bases
AAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAACTTTCGCTACGTTGCTGGCTC
GTTTTAACACGGAGTAAGTG

Downstream 100 bases:

>100_bases
CCTACACTAGCGGGATTCTTTTTGTTAACCCCTACCCCACGCGTACAACCGCGTGGGGAGACGACGCGGATTTTTAACTA
TGCGTATCCCCCGCATTTAT

Product: DNA-specific endonuclease I

Products: NA

Alternate protein names: Endonuclease I; Endo I

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPGTFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRV
EWEHVVPAWQFGHQRQCWQDGGRKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKVQGNHNPYVQRACQARKS

Sequences:

>Translated_235_residues
MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPGTFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRV
EWEHVVPAWQFGHQRQCWQDGGRKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKVQGNHNPYVQRACQARKS
>Mature_235_residues
MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPGTFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRV
EWEHVVPAWQFGHQRQCWQDGGRKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKVQGNHNPYVQRACQARKS

Specific function: Has double-strand break activity

COG id: COG2356

COG function: function code L; Endonuclease I

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endA/nucM nuclease family

Homologues:

Organism=Escherichia coli, GI1789314, Length=235, Percent_Identity=100, Blast_Score=494, Evalue=1e-141,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END1_ECOLI (P25736)

Other databases:

- EMBL:   X65169
- EMBL:   U28377
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   A38966
- RefSeq:   AP_003502.1
- RefSeq:   NP_417420.1
- ProteinModelPortal:   P25736
- SMR:   P25736
- STRING:   P25736
- EnsemblBacteria:   EBESCT00000002024
- EnsemblBacteria:   EBESCT00000002025
- EnsemblBacteria:   EBESCT00000015150
- GeneID:   949092
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2912
- KEGG:   eco:b2945
- EchoBASE:   EB1312
- EcoGene:   EG11336
- eggNOG:   COG2356
- GeneTree:   EBGT00050000009475
- HOGENOM:   HBG297976
- OMA:   ECKRDNA
- ProtClustDB:   PRK15137
- BioCyc:   EcoCyc:EG11336-MONOMER
- Genevestigator:   P25736
- InterPro:   IPR007346

Pfam domain/function: PF04231 Endonuclease_1

EC number: =3.1.21.1

Molecular weight: Translated: 26709; Mature: 26709

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPGTFYCGCKINWQGKKGV
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEEEECCCCEEEEEEEEEECCCCCE
VDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGGRKNCAKDPVYRKMESDMH
EEHHHCCEEECCCCCHHHHCCHHHCCCHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHH
NLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEKAAEPPARARGAIARTYFYMR
HCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCHHHCCCCCHHHHHHHHHHHEEEE
DQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKVQGNHNPYVQRACQARKS
CCCCEEECHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPGTFYCGCKINWQGKKGV
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEEEECCCCEEEEEEEEEECCCCCE
VDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGGRKNCAKDPVYRKMESDMH
EEHHHCCEEECCCCCHHHHCCHHHCCCHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHH
NLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEKAAEPPARARGAIARTYFYMR
HCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCHHHCCCCCHHHHHHHHHHHEEEE
DQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKVQGNHNPYVQRACQARKS
CCCCEEECHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7867949; 9278503; 4955462; 8113204