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The map label for this gene is ygfM

Identifier: 218696476

GI number: 218696476

Start: 3244501

End: 3245280

Strand: Direct

Name: ygfM

Synonym: EC55989_3167

Alternate gene names: 218696476

Gene position: 3244501-3245280 (Clockwise)

Preceding gene: 218696475

Following gene: 218696477

Centisome position: 62.94

GC content: 54.49

Gene sequence:

>780_bases
ATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTG
GTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTACAGGATCTGGAAC
TGGACTGGGTTGACTGGGATAACGGTGCACTGCGGATTGGCGCAATGTCTCGCTTGCAGCCACTGCGTGATGCGCGATTT
ATTCCTGCAGCGCTGCGTGAAGCCCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAAT
CGCCGCCCGCCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGTTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAA
CGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATCGCCTGCTAACCGAAATTATCATTAAAGATCCGTATCGCACC
TGTGCGACCCGCAAAATTAGCCGTTCTCAGGCAGGTTTAACCGTCGTGACGGCAGCCGTTGCAATGACAGACCACGACGG
TATGCGAATTGCGCTGGATGGCGTCGCCAGTAAAGCACTGCGTCTGCATGATGTCGAAAAACAAAATCTGGAAGGCAATG
CACTTGAACAGGCTGTCGCCAACGCCATTTTCCCGCAGGAAGATTTGCGGGGCAGCGTGGCCTATAAACGCTATATCACG
GGAGTTCTGGTAGCCGACCTGTATGCCGACTGCCAACAGGCTGGGGAGGAAGCCGTATGA

Upstream 100 bases:

>100_bases
CCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGA
GGGTTAAATAAGGAAAGAAG

Downstream 100 bases:

>100_bases
TCATCCACTTTACTTTAAATGGCGCGCCTCAGGAGCTAACCGTTAATCCAGGCGAAAACGTGCAAAAGCTGTTGTTTAAC
ATGGGAATGCACTCTGTACG

Product: putative selenate reductase subunit YgfM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKKIAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARF
IPAALREALGFVYSRHVRNQSTIGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQAVANAIFPQEDLRGSVAYKRYIT
GVLVADLYADCQQAGEEAV

Sequences:

>Translated_259_residues
MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKKIAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARF
IPAALREALGFVYSRHVRNQSTIGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQAVANAIFPQEDLRGSVAYKRYIT
GVLVADLYADCQQAGEEAV
>Mature_259_residues
MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKKIAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARF
IPAALREALGFVYSRHVRNQSTIGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQAVANAIFPQEDLRGSVAYKRYIT
GVLVADLYADCQQAGEEAV

Specific function: Unknown

COG id: COG1319

COG function: function code C; Aerobic-type carbon monoxide dehydrogenase, middle subunit CoxM/CutM homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain

Homologues:

Organism=Escherichia coli, GI1789245, Length=259, Percent_Identity=100, Blast_Score=529, Evalue=1e-152,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YGFM_ECO57 (P64558)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A98098
- PIR:   E85943
- RefSeq:   NP_289450.1
- RefSeq:   NP_311780.1
- ProteinModelPortal:   P64558
- EnsemblBacteria:   EBESCT00000024136
- EnsemblBacteria:   EBESCT00000058951
- GeneID:   916427
- GeneID:   958366
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4219
- KEGG:   ecs:ECs3753
- GeneTree:   EBGT00050000008228
- HOGENOM:   HBG467251
- OMA:   TEIRIPI
- ProtClustDB:   PRK09799
- BioCyc:   ECOL83334:ECS3753-MONOMER
- InterPro:   IPR005107
- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR002346
- InterPro:   IPR017698
- Gene3D:   G3DSA:3.30.390.50
- Gene3D:   G3DSA:3.30.465.10
- Gene3D:   G3DSA:3.30.43.10
- TIGRFAMs:   TIGR03312

Pfam domain/function: PF03450 CO_deh_flav_C; PF00941 FAD_binding_5; SSF55447 CO_deh_flav_C; SSF56176 FAD-binding_2

EC number: NA

Molecular weight: Translated: 28650; Mature: 28650

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS51387 FAD_PCMH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKKIAISLQDLELDWVDWD
CCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEEEEEEEEEEEEECC
NGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQSTIGGEIAARQEESVLLPVL
CCEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCHHHHHH
LALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRTCATRKISRSQAGLTVVTAAV
HHHCCEEEECCCCEEEEHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHH
AMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQAVANAIFPQEDLRGSVAYKRYIT
HEECCCCCEEEECHHHHHHHHHHHCHHCCCCCHHHHHHHHHHCCCHHHHCCHHHHHHHHH
GVLVADLYADCQQAGEEAV
HHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKKIAISLQDLELDWVDWD
CCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEEEEEEEEEEEEECC
NGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQSTIGGEIAARQEESVLLPVL
CCEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCHHHHHH
LALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRTCATRKISRSQAGLTVVTAAV
HHHCCEEEECCCCEEEEHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHH
AMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQAVANAIFPQEDLRGSVAYKRYIT
HEECCCCCEEEECHHHHHHHHHHHCHHCCCCCHHHHHHHHHHCCCHHHHCCHHHHHHHHH
GVLVADLYADCQQAGEEAV
HHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796