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The map label for this gene is csdA

Identifier: 218696409

GI number: 218696409

Start: 3160650

End: 3161855

Strand: Direct

Name: csdA

Synonym: EC55989_3089

Alternate gene names: 218696409

Gene position: 3160650-3161855 (Clockwise)

Preceding gene: 218696404

Following gene: 218696410

Centisome position: 61.31

GC content: 55.89

Gene sequence:

>1206_bases
ATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCGTCTATCTCGACAGCGCCGC
GACCGCGCTTAAACCTGAAGCCGTGGTTGAAGCCACCCAACAGTTTTACAGTCTGAGCGCCGGAAACGTCCATCGCAGCC
AGTTTGCCGAAGCCCAACGCCTGACCGCGCGTTATGAAGCTGCACGAGAGAAAGTGGCGCAATTACTGAATGCACCGGAT
GATAAAACTATCGTCTGGACGCGCGGCACCACTGAATCCATCAACATGGTGGCACAATGCTATGCGCGTCCGCGTCTGCA
ACCGGGCGATGAGATTATTGTCAGCGTGGCAGAACACCACGCCAACCTCGTCCCCTGGCTGATGGTCGCCCAACAAACTG
GAGCCAAAGTGGTGAAATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGT
AGTCGGATTCTGGCGTTGGGTCAGATGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCA
TTCAGCCGGGATGGTGGTGATGGTTGATGGTGCTCAGGGGGCAGTGCATTTCCCCGCGGATGTTCAGCAACTGGATATTG
ATTTCTATGCTTTTTCAGGTCACAAACTGTATGGCCCGACAGGTATCGGCGTGCTGTATGGTAAATCAGAACTGCTGGAG
GCGATGTCGCCCTGGCTGGGCGGCGGCAAAATGGTTCACGAAGTGAGTTTTGACGGCTTCACGACTCAATCTGCGCCGTG
GAAACTGGAAGCTGGAACGCCAAATGTCGCTGGTGTCATAGGATTAAGCGCGGCGCTGGAATGGCTGGCAGATTACGATA
TCAACCAGGCCGAAAGCTGGAGCCGTAGCTTAGCAACGCTGGCGGAAGATGCGCTGGCGAAACGTCCCGGCTTTCGTTCA
TTCCGCTGCCAGGATTCCAGCCTGCTGGCCTTTGATTTTGCTGGCGTTCATCATAGCGATATGGTGACGCTGCTGGCGGA
GTACGGTATTGCCCTGCGGGCCGGGCAGCATTGCGCTCAGCCGCTACTGGCAGAATTAGGCGTAACCGGCACACTGCGCG
CCTCTTTTGCGCCATATAATACAAAGAGTGATGTGGATGCGCTGGTGAATGCCGTTGACCGCGCGCTGGAATTATTGGTG
GATTAA

Upstream 100 bases:

>100_bases
GCGGCTTCGCCAGGATATCCAGATAATTCTGATGGTTAGCACTCTCCTTGTATCAAAGTGAATTTTGCGTCACGATCGGT
GCATCAAGCCGAGGAGTACC

Downstream 100 bases:

>100_bases
TGACAAACCCGCAATTCGCCGGACATCCGTTCGGCACAACCGTAACCGCAGAAACGTTACGCAATACCTTCGCACCGTTG
TCGCAATGGGAAGATAAATA

Product: cysteine sulfinate desulfinase

Products: @ALAN01txt*L-Alanine! [C]

Alternate protein names: CSD

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATQQFYSLSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPD
DKTIVWTRGTTESINMVAQCYARPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAFSGHKLYGPTGIGVLYGKSELLE
AMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNVAGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRS
FRCQDSSLLAFDFAGVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAVDRALELLV
D

Sequences:

>Translated_401_residues
MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATQQFYSLSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPD
DKTIVWTRGTTESINMVAQCYARPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAFSGHKLYGPTGIGVLYGKSELLE
AMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNVAGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRS
FRCQDSSLLAFDFAGVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAVDRALELLV
D
>Mature_401_residues
MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATQQFYSLSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPD
DKTIVWTRGTTESINMVAQCYARPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAFSGHKLYGPTGIGVLYGKSELLE
AMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNVAGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRS
FRCQDSSLLAFDFAGVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAVDRALELLV
D

Specific function: Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L- selenocystine to produce L-alanine. L-cysteine sulfinic acid is the best substrate. Functions as a selenium delivery protein in the pathway f

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily

Homologues:

Organism=Homo sapiens, GI32307132, Length=392, Percent_Identity=25.765306122449, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI156713448, Length=434, Percent_Identity=25.1152073732719, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789175, Length=401, Percent_Identity=100, Blast_Score=824, Evalue=0.0,
Organism=Escherichia coli, GI1787970, Length=404, Percent_Identity=43.0693069306931, Blast_Score=327, Evalue=8e-91,
Organism=Escherichia coli, GI48994898, Length=215, Percent_Identity=30.2325581395349, Blast_Score=92, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI25143064, Length=229, Percent_Identity=29.2576419213974, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI193211090, Length=384, Percent_Identity=25, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319831, Length=401, Percent_Identity=27.431421446384, Blast_Score=103, Evalue=6e-23,
Organism=Drosophila melanogaster, GI20129463, Length=360, Percent_Identity=27.7777777777778, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CSDA_ECOLI (Q46925)

Other databases:

- EMBL:   AX000470
- EMBL:   U29581
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   F65063
- RefSeq:   AP_003376.1
- RefSeq:   NP_417290.1
- ProteinModelPortal:   Q46925
- SMR:   Q46925
- DIP:   DIP-9323N
- IntAct:   Q46925
- STRING:   Q46925
- PRIDE:   Q46925
- EnsemblBacteria:   EBESCT00000002613
- EnsemblBacteria:   EBESCT00000014307
- GeneID:   947275
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2781
- KEGG:   eco:b2810
- EchoBASE:   EB2891
- EcoGene:   EG13082
- eggNOG:   COG0520
- GeneTree:   EBGT00050000010781
- HOGENOM:   HBG635316
- OMA:   PWQGGGK
- ProtClustDB:   PRK10874
- BioCyc:   EcoCyc:G7454-MONOMER
- BioCyc:   MetaCyc:G7454-MONOMER
- Genevestigator:   Q46925
- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR022471
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR03392

Pfam domain/function: PF00266 Aminotran_5; SSF53383 PyrdxlP-dep_Trfase_major

EC number: 4.4.1.- [C]

Molecular weight: Translated: 43235; Mature: 43235

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: ACT_SITE 358-358

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATQQFYSLSAGNVHRSQFAEAQR
CCCCCCHHHHHHCCCHHCCCEEECCHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYARPRLQPGDEIIVSVAEHH
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
ANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPRSRILALGQMSNVTGGCPDLA
CCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHCCHHHEEEHHCCCCCCCCCHHHH
RAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAFSGHKLYGPTGIGVLYGKSELLE
HHHHHHHCCCEEEEEECCCCCEECCCCCEEEEEEEEEECCCEEECCCCCEEEECHHHHHH
AMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNVAGVIGLSAALEWLADYDINQAESW
HHCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
SRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFAGVHHSDMVTLLAEYGIALRAGQHCAQ
HHHHHHHHHHHHHHCCCCCCEECCCCCEEEEEECCCCHHHHHHHHHHCCHHHHCCHHHHH
PLLAELGVTGTLRASFAPYNTKSDVDALVNAVDRALELLVD
HHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATQQFYSLSAGNVHRSQFAEAQR
CCCCCCHHHHHHCCCHHCCCEEECCHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
LTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYARPRLQPGDEIIVSVAEHH
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
ANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPRSRILALGQMSNVTGGCPDLA
CCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHCCHHHEEEHHCCCCCCCCCHHHH
RAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAFSGHKLYGPTGIGVLYGKSELLE
HHHHHHHCCCEEEEEECCCCCEECCCCCEEEEEEEEEECCCEEECCCCCEEEECHHHHHH
AMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNVAGVIGLSAALEWLADYDINQAESW
HHCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
SRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFAGVHHSDMVTLLAEYGIALRAGQHCAQ
HHHHHHHHHHHHHHCCCCCCEECCCCCEEEEEECCCCHHHHHHHHHHCCHHHHCCHHHHH
PLLAELGVTGTLRASFAPYNTKSDVDALVNAVDRALELLVD
HHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-cystine; L-selenocystine [C]

Specific reaction: Catalyzes the removal of elemental sulfur and selenium atoms from l-cysteine, l-cystine, l-selenocysteine, and l- selenocystine to produce @ALAN01.txt*l-Alanine! [C]

General reaction: Remove sulfur and selenium atoms [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278392; 9278503; 10829016; 10739946