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The map label for this gene is hcaF

Identifier: 218696166

GI number: 218696166

Start: 2893743

End: 2894261

Strand: Direct

Name: hcaF

Synonym: EC55989_2825

Alternate gene names: 218696166

Gene position: 2893743-2894261 (Clockwise)

Preceding gene: 218696165

Following gene: 218696167

Centisome position: 56.14

GC content: 52.41

Gene sequence:

>519_bases
ATGAGTGCGCAAGTTTCACTAGAGTTACATCACCGCATTAGCCAGTTTCTCTTTCACGAAGCCAGCTTACTGGACGACTG
GAAATTTCGTGACTGGCTGGCGCAGCTCGACGAAGAGATTCGTTACACCATGCGCACCACAGTTAACGCGCAAACACGCG
ACCGCCGCAAAGGCGTCCAGCCACCGACAACCTGGATTTTTAATGACACCAAAGACCAGCTGGAGCGGCGAATCGCCCGT
CTGGAAACGGGCATGGCATGGGCAGAAGAGCCGCCGTCACGCACCCGCCACTTAATCAGCAACTGCCAGATAAGCGAAAC
CGACATCCCAAACGTATTTGCTGTTCGGGTGAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACGTTCTATG
TCGGGACTCGTTTTGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAA
GCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGA

Upstream 100 bases:

>100_bases
CCGCTCGTGGAATGTACCAACGCTGGGCCGATCTTCTGAGTAGCGAAAGCTGGCAGGAAGTGCTCGATAAAACCGCCGCT
TACCAGCAGGAGGTGATGAA

Downstream 100 bases:

>100_bases
TGAATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGTGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCC
CTGTTCAACGTTGGCGGCGA

Product: 3-phenylpropionate dioxygenase subunit beta

Products: NA

Alternate protein names: Digoxigenin subunit beta

Number of amino acids: Translated: 172; Mature: 171

Protein sequence:

>172_residues
MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTMRTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIAR
LETGMAWAEEPPSRTRHLISNCQISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
AVITSHNLSVLF

Sequences:

>Translated_172_residues
MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTMRTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIAR
LETGMAWAEEPPSRTRHLISNCQISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
AVITSHNLSVLF
>Mature_171_residues
SAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTMRTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARL
ETGMAWAEEPPSRTRHLISNCQISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQA
VITSHNLSVLF

Specific function: Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively

COG id: COG5517

COG function: function code Q; Small subunit of phenylpropionate dioxygenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family

Homologues:

Organism=Escherichia coli, GI1788889, Length=172, Percent_Identity=100, Blast_Score=353, Evalue=2e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HCAF_ECO24 (A7ZPY2)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001463861.1
- ProteinModelPortal:   A7ZPY2
- SMR:   A7ZPY2
- STRING:   A7ZPY2
- EnsemblBacteria:   EBESCT00000020281
- GeneID:   5589475
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_2824
- eggNOG:   COG5517
- GeneTree:   EBGT00050000011518
- HOGENOM:   HBG582498
- OMA:   DIFAGER
- ProtClustDB:   PRK10069
- BioCyc:   ECOL331111:ECE24377A_2824-MONOMER
- HAMAP:   MF_01649
- InterPro:   IPR000391

Pfam domain/function: PF00866 Ring_hydroxyl_B

EC number: =1.14.12.19

Molecular weight: Translated: 20579; Mature: 20448

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTMRTTVNAQTRDRRKGVQ
CCCEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCC
PPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNCQISETDIPNVFAVRVNYL
CCCEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
LYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQAVITSHNLSVLF
HHHHHHCCCCEEEEECHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure 
SAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTMRTTVNAQTRDRRKGVQ
CCEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCC
PPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNCQISETDIPNVFAVRVNYL
CCCEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
LYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQAVITSHNLSVLF
HHHHHHCCCCEEEEECHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA